Gallamine

Manufactured by Merck Group

Sourced in United States

Gallamine is a laboratory equipment product manufactured by Merck Group. It is used as a neuromuscular blocking agent in medical research settings.

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Lab products found in correlation

Pirenzepine, by Bio-Techne (1 mentions) 4 damp, by Bio-Techne (1 mentions) Celltiter 96 aqueus one solution cell proliferation assay, by Promega (1 mentions) Urethane, by Merck Group (1 mentions) 5 ht creatinine sulfate, by Merck Group (1 mentions) Phenylephrine hydrochloride, by Merck Group (1 mentions) Ketanserin, by Merck Group (1 mentions) Ascorbate, by Merck Group (1 mentions) Hexamethonium, by Merck Group (1 mentions) Noradrenaline hydrochloride, by Merck Group (1 mentions) Enrofloxacin, by Bayer (1 mentions)

Spelling variants of this product

Gallamine triethiodide (15 citations)

4 protocols using gallamine

CellMinerCDB Toxicity Analysis Protocol

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CellMinerCDB analyses were performed using the publicly accessible web portal https://discover.nci.nih.gov/rsconnect/cellminercdb/ (see Ref. [29] (link) and refs. cited therein). The gene expression data were exported to Excel for comparison of the average toxicity features of the 10 vs. 50 cell lines exhibiting striking difference in HIST1H1B mRNA levels, and the drugs differentiating best between the two categories of cells based on the calculated means and variances were further analyzed experimentally as described in Results and Discussion. These drugs (Gallamine, Hinokitiol) were obtained through the courtesy of the NIH Developmental Therapeutics Program, while ß-Lapachone was purchased from Sigma-Aldrich.

Imre L., Niaki E.F., Bosire R., Nanasi P J.r., Nagy P., Bacso Z., Hamidova N., Pommier Y., Jordan A, & Szabo G. (2022). Nucleosome destabilization by polyamines. Archives of biochemistry and biophysics, 722, 109184.

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Muscarinic Receptor Modulation on Cell Proliferation

Cited 1 time

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The M2 agonist Arecaidine was purchased from Sigma-Aldrich. The muscarinic antagonists used were: gallamine, (final concentration 10⁻⁶M, Sigma, Cat. N. G-8134) as M2 antagonist, pirenzepine (final concentration 10⁻⁶M, Tocris, Cat. N. 1071) as M1 antagonist and 4-DAMP (final concentration 10⁻⁸M, Tocris, Cat. N. 0482) as M3 antagonist.²⁸ (link) Cell growth of T24 cell lines (2000 cells/well) and human dermal fibroblasts^41,42 was quantified using a colorimetric method (CellTiter 96 AQueus One Solution Cell Proliferation Assay, Promega) according to the manufacturer's instructions.^42,43 (link)

Pacini L., De Falco E., Di Bari M., Coccia A., Siciliano C., Ponti D., Pastore A.L., Petrozza V., Carbone A., Tata A.M, & Calogero A. (2014). M2muscarinic receptors inhibit cell proliferation and migration in urothelial bladder cancer cells. Cancer Biology & Therapy, 15(11), 1489-1498.

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Neurotransmitter Receptor Pharmacology

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Materials were purchased from US companies and solubilized in the carrier noted in parentheses. 5-carboxamidotryptamine maleate (water), 5-HT creatinine sulfate (water), ascorbate (water), gallamine, hexamethonium, ketanserin (DMSO, used dilutions in water), noradrenaline hydrochloride (water), phenylephrine hydrochloride (water) and urethane were obtained from Sigma Chemical Company (St. Louis, MO, USA). 8-OH-DPAT (water), CP93129 (water), TTX (water) and UK14304 (dimethylsulfoxide) were purchased from Tocris (R&D systems, Minneapolis, MN, USA). Fatal Plus (Pentobarbital 390 mg/ml) was purchased from Vortech Pharmaceutical (Dearborn MI USA). Isoflurane (IsofloTM) was purchased from Abbott (USA) and Enrofloxacin from Bayer Healthcare LLC (USA).

Darios E.S., Barman S.M., Orer H.S., Morrison S.F., Davis R.P., Seitz B.M., Burnett R, & Watts S.W. (2015). 5-Hydroxytryptamine does not reduce sympathetic nerve activity or neuroeffector function in the splanchnic circulation. European journal of pharmacology, 754, 140-147.

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Arteriole Vasoconstriction Measurement Protocol

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Arteriole diameter measurement was obtained with live video microscopy. The vessel’s internal diameters were measured from wall to wall by a video dimension micrometer. The video micrometer was calibrated on-screen with a stage micrometer scale before the start of each experiment. To eliminate the confounding effect of vascular response to the altered metabolic rate by toxin-mediated muscle paralysis, gallamine triethiodide (gallamine, 0.17 nM; Sigma-Aldrich Co.), an acetylcholine receptor antagonist, was added into the perfusate for 10 min. Although gallamine did not cause significant changes in vessel diameter, baseline measurement was done after the addition of gallamine. The electrical stimulation-induced cremaster arteriole vasoconstriction model (30 V, 16 Hz, 5 s) was used to mimic the arteriole vasoconstriction with intervals of 5 min. The diameters of cremaster arteriole were recorded and the arteriole diameter constriction rate was established by the following equation: arteriole diameter shrinkage rate = [diameter of arteriole before electrical stimulation–diameter of arteriole after electrical stimulation]/diameter of arteriole before electrical stimulation ×100%.

Zhou Y., Liu Y., Hao Y., Feng Y., Pan L., Liu W., Li B., Xiao L., Jin L, & Nie Z. (2018). The mechanism of botulinum A on Raynaud syndrome. Drug Design, Development and Therapy, 12, 1905-1915.

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