Are 250

Manufactured by Thinky

Sourced in Japan

The ARE-250 is a laboratory mixing machine designed for blending, kneading, and dispersing a variety of materials. It features a stainless-steel mixing bowl and a high-speed agitator that can accommodate batch sizes up to 250 milliliters. The machine is equipped with digital controls for adjusting the speed and time of the mixing process.

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Lab products found in correlation

Tipure r960, by DuPont (1 mentions) Sylgard 184, by Merck Group (1 mentions) Silver powder, by Merck Group (1 mentions) Black pearls 2000, by Cabot (1 mentions) 24 well plate, by GeneDireX (1 mentions) Proroot mta, by Dentsply (1 mentions) Ammonium phosphate dibasic, by Merck Group (1 mentions) Polyvinylpyrrolidone, by Merck Group (1 mentions) Pentanol, by Merck Group (1 mentions) Omnicure s1000, by Excelitas (1 mentions) Lignin, by Merck Group (1 mentions) Glycerol, by Merck Group (1 mentions) Avanti j e, by Beckman Coulter (1 mentions) Sylgard 184, by Dow (1 mentions)

Close spelling variants of this product

ARE-250 mixer (6 citations)

27 protocols using are 250

Preparation of Acrylic Resin Compound

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Example 24

A pigment pre-mixture, which comprises H-1/TiO2 (available as TiPureR960, from DuPont)/methyl-isobutyl-ketone (MIBK)=10:50:40 by weight, was prepared. Said acrylic resin compound was mixed with the paint shaker (ARE250, provided by Thinky) for 10 minutes. The ratio of Hard Polymer1, Soft Polymer1 and the TiO2 was 100:100:50 by weight. The compatibility of said polymers and pigment was judged as “Good”.

Said polymer solution was coated onto 50 μm release polyester film with a knife coater. Then said coated layer was dried and cross-linked for 3 minutes at 95 degree C. and for 2 minutes at 155° C. After drying, 50 μm acrylic film layer was obtained. Then said white film was laminated with same adhesive as Example 22.

US10358582B2. Marking film (2019-07-23). None [JP], None [JP], None [US], None [JP], None [US]. Inventors: Hidetoshi Abe [JP], Yorinobu Takamatsu [JP], Ronald S. Steelman [US], Naoyuki Toriumi [JP], Michael P. Daniels [US].

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Fabrication of Conductive BCP Ink

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The ink is prepared by dissolving SIS in toluene (15 wt% SIS) until a clear solution is obtained. For each 5 g of BCP solution, 6.2 g of Ag flakes (Silflake, Technic) and 15 g of EGaIn are added and mixed using a planetary mixer (Thinky ARE-250) at 2000 r.p.m.

Lopes P.A., Santos B.C., de Almeida A.T, & Tavakoli M. (2021). Reversible polymer-gel transition for ultra-stretchable chip-integrated circuits through self-soldering and self-coating and self-healing. Nature Communications, 12, 4666.

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Fabrication of PDMS Microfluidic Devices

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We weight approximately 60 g of 5:1 RTV 615 and 21 g of 20:1 RTV 615. We use a Thinky ARE-250 mixer 2000 RPM mixing and 2 min at 2000 RPM degassing. We make an aluminum foil dish for the wafer. We pour the 5:1 PDMS onto the fluid layer and degas one last time to remove bubbles in the photoresist for approximately 10 min. We then spin coat the 20:1 PDMS at 2000 RPM for 30 seconds. The 5:1 layer is baked for 1 hour at 80°C and the spin-coated layer is baked for 40 minutes at 80°C. The thick layer is cooled off and cut into pieces and holes are punched (0.5mm puncher for the flow inlets and 1.25mm for the root inlets). Scotch tape is then applied to remove particles, and the pieces are aligned by hand under the stereomicroscope. An additional 2 hours of bonding is done at 80°C in the oven. The pieces are then cut out and the final holes for the control valves are punched out (0.5 mm puncher). Scotch tape is applied. Glass slides are prepared by sonication briefly in isopropanol. The devices are plasma bonded using 1 min medium power in a Harrick plasma cleaner (pdc-02). After sealing, the devices they are baked at 100°C for 1 hour on a hotplate and then placed back in the oven overnight at 80°C and then are ready to go and solidly bonded.

Fendrych M., Akhmanova M., Merrin J., Glanc M., Hagihara S., Takahashi K., Uchida N., Torii K.U, & Friml J. (2018). Rapid and reversible root growth inhibition by TIR1 auxin signalling. Nature plants, 4(7), 453-459.

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Carbonization of TOCNF-LCNF Composites

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Aqueous
suspensions of TOCNF of a given composition (0, 5, 13, 20, 33, and
100% TOCNF, based on total dry mass) was added to 50 g of 6.1% LCNF.
The mixed dopes were stirred (3 min at 2000 rpm) and degassed (3 min
at 2500 rpm) via a planetary centrifugal mixer (THINKY ARE-250). The
prepared suspensions or dopes were loaded into a 50 mL plastic syringe
connected with a plastic tube (44.5 cm in length and 6 mm in inner
diameter) that ended in a needle with an inner diameter of 1.2 mm
and a length of 3.7 cm. The latter was immersed in a coagulation bath
(acetone) during spinning at a fixed rate of 10 mL min^–1. After approximately 1 min, filaments were collected from the acetone
antisolvent and dried under tension under room temperature (23 °C)
conditions. The wet-spun microfibers contained the given TOCNF amount,
therein referred to as LCNF, L/T5, L/T13, L/T20, L/T33, and TOCNF,
respectively. The wet-spun microfibers were carbonized in a tubular
furnace (NBD-O1200–50IC Vacuum Tube, Furnace) at 900 °C
for 60 min operated at a heating rate of 2 °C min^–1 under a N₂ flux. After carbonization, carbon microfibers
were obtained and named following the same nomenclature as before
but adding a subscript, the letter “c” standing for
carbonization, namely, LCNFc, L/T5c, L/T13c, L/T20c, L/T33c, and TOCNFc.
The carbonization yield was calculated from the mass before and after
carbonization.

Wang L., Borghei M., Ishfaq A., Lahtinen P., Ago M., Papageorgiou A.C., Lundahl M.J., Johansson L.S., Kallio T, & Rojas O.J. (2020). Mesoporous Carbon Microfibers for Electroactive Materials Derived from Lignocellulose Nanofibrils. ACS Sustainable Chemistry & Engineering, 8(23), 8549-8561.

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Fabrication of Ag-PDMS Composites

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To fabricate the Ag-PDMS composites, the PDMS prepolymer (Sylgard 184) and silver powder, 2–3.5 micron, 99.9+% (Sigma Aldrich) were first dispersed by hand using a spatula and then mixed in a planetary mixer (Thinky ARE-250) for 3 minutes at 2000 rpm and degassed for 1 minute at 2200 rpm. Next, PDMS curing agent was added (respecting a 10:1 ratio between the prepolymer and curing agent) and incorporated into the mixture by stirring manually before mixing again for 1.5 minute at 2000 rpm and degassing for 30 seconds at 2200 rpm. The viscous paste that was obtained was then stored in a freezer at −24°C until usage. Prior to usage, the paste was manually stirred with a spatula and mixed for 1 minute at 2000 rpm and degassed for 30 seconds at 2200 rpm.

Larmagnac A., Eggenberger S., Janossy H, & Vörös J. (2014). Stretchable electronics based on Ag-PDMS composites. Scientific Reports, 4, 7254.

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Fabrication of Conductive PDMS Electrodes

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The compliant electrodes were manufactured by mask-spraying with an airbrush a PDMS-carbon black composite ink. The ink was obtained by mixing in a high speed planetary mixer (ARE250, Thinky, USA) a PDMS silicone pre-polymer (MED4910, Nusil, USA) with 9 wt% of carbon black (Black Pearls 2000, Cabot, USA) and solvents (isopropanol, isooctane). The sprayed mixture was cross-linked on the elastomer membrane in an oven at 80 °C for 45 minutes.

Ghilardi M., Boys H., Török P., Busfield J.J, & Carpi F. (2019). Smart Lenses with Electrically Tuneable Astigmatism. Scientific Reports, 9, 16127.

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Robocasting Ink Preparation Using Pluronic F-127

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Hydrogels containing 33 wt.% Pluronic F-127 in deionized water were chosen as processing media for the preparation of the robocasting inks. Two different volumes were prepared at a time: 5 mL and 10 mL stock solutions. Zirconia balls were added to both solutions to aid in the mixing process: 3 balls with a 5 mm diameter to the 5 mL stock, 6 balls with 5 mm, and an additional 3 balls with 10 mm diameter to the 10 mL stock solution. Pastes were produced by adding the powders to the Pluronic F-127 hydrogels and mixing in a planetary centrifugal mixer (ARE-250, Thinky Corp., Tokyo, Japan) at different RPM, resulting in homogenized inks.

Bento R., Gaddam A., Oskoei P., Oliveira H, & Ferreira J.M. (2021). 3D Printing of Macro Porous Sol-Gel Derived Bioactive Glass Scaffolds and Assessment of Biological Response. Materials, 14(20), 5946.

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Fabrication of CS/β-TCP Bone Scaffolds

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CS and β-TCP with particle sizes less than 38 μm were purchased from Kunshan Chinese Technology New Materials Co., Ltd. (Shanghai, China). β-TCP powders doped with 0%, 5%, 10%, 20%, 30%, 40%, and 50% CS (wt.%) were prepared in batches of 5 g, and mixed at 1,500 rpm for 30 min using a hybrid defoaming mixer (ARE-250, Thinky, Japan) as described previously^{7 (link)}. Injectable powders for printing were prepared by combining 5 g of mixed powder with 3 g of polyvinyl alcohol solution (6 wt.%). Square scaffolds (7 mm × 7 mm × 3.5 mm) with interconnected 500 μm square channels were designed in CAD, allowing for approximately 30% shrinkage^{7 (link), 39 (link)}. The scaffolds were 3D-printed (Dresden, Germany) as described previously, dried at room temperature for 24 h, and sintered at 1,100 °C for 2 h^{6 (link)}. The scaffolds obtained (5 mm × 5 mm × 2.5 mm) were sterilized using ethylene oxide^{17 (link), 46 (link)}, and halves (5 mm × 2.5 mm × 2.5 mm) were used in transwell and animal experiments.

Deng Y., Jiang C., Li C., Li T., Peng M., Wang J, & Dai K. (2017). 3D printed scaffolds of calcium silicate-doped β-TCP synergize with co-cultured endothelial and stromal cells to promote vascularization and bone formation. Scientific Reports, 7, 5588.

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Resorcinol-Formaldehyde Oligomer Synthesis

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Next, 16 g of the prepared resorcinol-formaldehyde oligomer was dissolved in 80 g of NMP using a high shear force mixer (Thinky ARE-250, Tokyo, Japan) at 1600 rpm for 30 min. The mixing cycle was repeated twice; between cycles, mixing was stopped for 15 min to prevent the solution from overheating. Next, 2.8 g of formaldehyde was introduced to the solution and mixed at 2000 rpm for 10 min. Then, 0.1 g of KOH was added and the solution mixed for 30 min at 1600 rpm. Finally, the whole solution was defoamed via the Thinky mixer for 30 min at 2000 rpm to remove any bubble.

Rahimalimamaghani A., Pacheco Tanaka D.A., Llosa Tanco M.A., Neira D’Angelo M.F, & Gallucci F. (2022). Ultra-Selective CMSMs Derived from Resorcinol-Formaldehyde Resin for CO2 Separation. Membranes, 12(9), 847.

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Synthesis and Characterization of Calcium Silicate Cements

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White-coloured ProRoot MTA (Dentsply, Tulsa, OK, USA) and CS (composition: 65% CaO, 25% SiO₂, 5% Al₂O₃ and 5% ZnO) cements^{8 (link)} were used in present study. In brief, appropriate amounts of as-received SiO₂, CaO, Al₂O₃ and ZnO powders were mixed at 1 000 r⋅min⁻¹ for 10 min using a hybrid-defoaming mixer (ARE-250; Thinky, Tokyo, Japan). The oxide mixtures were then sintered at 1 400 °C for 2 h using a high-temperature furnace. The sintered granules were then ball-milled in ethyl alcohol using a centrifugal ball mill (S 100; Retsch, Hann, Germany) for 6 h. The CS powder (0.6 g) was mixed using a liquid/powder ratio of 0.35 mL⋅g⁻¹. After mixing, the cement fully covered each well of the 24-well plate (GeneDireX, Las Vegas, NV, USA) to a thickness of 2 mm, and the plate was stored in an incubator at 100% relative humidity and 37 °C for 1 day of hydration. According to the MTA manufacturer's instructions, a liquid/powder ratio of 0.3 mL⋅g⁻¹ was used to mix the cement. Before the experiments, all specimens were sterilised via soaking in a 75% ethanol solution and exposure to ultraviolet light for 1 h.

Lai W.Y., Kao C.T., Hung C.J., Huang T.H, & Shie M.Y. (2014). An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements. International Journal of Oral Science, 6(2), 94-98.

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