L phenylalanine

Manufactured by Fujifilm

Sourced in Japan

L-phenylalanine is a naturally occurring amino acid that serves as a building block for proteins. It is a colorless, crystalline powder that is commonly used in various laboratory applications, such as cell culture media and biochemical assays. L-phenylalanine is a key component in the production of essential biological compounds and plays a role in various metabolic processes.

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Lab products found in correlation

L valine, by Fujifilm (5 mentions) L methionine, by Fujifilm (5 mentions) L isoleucine, by Fujifilm (5 mentions) L alanine, by Fujifilm (4 mentions) L leucine, by Fujifilm (4 mentions) L threonine, by Fujifilm (4 mentions) L serine, by Fujifilm (3 mentions) L glycine, by Fujifilm (3 mentions) L tryptophan, by Fujifilm (3 mentions) L tyrosine, by Fujifilm (3 mentions) L glutamine, by Fujifilm (3 mentions) L histidine, by Fujifilm (3 mentions) L asparagine monohydrate, by Fujifilm (2 mentions) L aspartic acid, by Fujifilm (2 mentions) L glutamic acid, by Fujifilm (2 mentions) L proline, by Fujifilm (2 mentions) L arginine, by Fujifilm (2 mentions) L asparagine, by Fujifilm (2 mentions) L lysine, by Fujifilm (2 mentions) D fructose, by Fujifilm (1 mentions)

6 protocols using l phenylalanine

Taste Receptor Activation by Amino Acids

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Agar (purified powder) was obtained from Sigma-Aldrich (St Louis, MO, USA). As tastants, D-fructose (CAS: 57-48-7, Wako Pure Chemical Industries, Ltd., Osaka, Japan), as well as L-alanine (ALA; CAS: 56-41-7), L-arginine monohydrochloride (ARG; CAS: 1119-34-2), L-asparagine monohydrate (ASN; CAS: 5794-13-8), L-aspartic acid (ASP; CAS: 56-84-8), L-cysteine monohydrochloride hydrate (CYS; CAS: 7048-04-6), L-histidine monohydrochloride (HIS; CAS: 645-35-2), L-isoleucine (ILE; CAS: 73-32-5), L-leucine (LEU; CAS: 61-90-5), L-lysine monohydrochloride (LYS; CAS: 657-27-2), L-glutamine (GLN; CAS: 56-85-9), L-glutamic acid (GLU; CAS: 56-86-0), L-glycine (GLY; CAS: 56-40-6), L-methionine (MET; CAS: 63-68-3), L-phenylalanine (PHE; CAS: 63-91-2), L-proline (PRO; CAS: 147-85-3), L-serine (SER; CAS: 56-45-1), L-threonine (THR; CAS: 72-19-5), L-tryptophan (TRP; CAS: 73-22-3), L-tyrosine (TYR; CAS: 60-18-4) and L-valine (VAL; CAS: 72-18-4) were used at concentrations of 10 mmol l⁻¹ (TYR was used in 0.5 mmol l⁻¹ due to low solubility). Amino acids were obtained from Wako Pure Chemical Industries, Ltd., Osaka, Japan (TYR, SER, CYS, VAL), Sigma-Aldrich, St Louis, MO, USA (ILE, PRO) or Nacalai Tesque, Kyoto, Japan (ALA, ARG, ASN, ASP, LEU, LYS, GLN, GLY, MET, PHE, THR, TRP, TYR).

Kudow N., Miura D., Schleyer M., Toshima N., Gerber B, & Tanimura T. (2017). Preference for and learning of amino acids in larval Drosophila. Biology Open, 6(3), 365-369.

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Synthesis and Analysis of Amino Acids

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L-Kynurenine sulfate salt, KYNA, and L-tyrosine disodium salt hydrate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). L-Alanine, L-arginine, L-asparagine, L-aspartate, L-cysteine hydrochloride monohydrate, L-glutamine, L-glutamate, L-glycine, L-histidine, L-isoleucine, L-leucine hydrochloride, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, and L-valine were purchased from Wako Pure Chemical Industries (Osaka, Japan). Rodent diet (MF) was obtained from Oriental Yeast Co., Ltd (Tokyo, Japan). All other chemicals were of the highest commercially available purity.

Sekine A., Okamoto M., Kanatani Y., Sano M., Shibata K, & Fukuwatari T. (2015). Amino acids inhibit kynurenic acid formation via suppression of kynurenine uptake or kynurenic acid synthesis in rat brain in vitro. SpringerPlus, 4, 48.

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Analytical-Grade Chemical Compounds

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Propionic acid (PA), butyric acid (BA), L-glycine (Gly), L-alanine (Ala), L-serine (Ser), L-threonine (Thr), L-cysteine (Cys), L-methionine (Met), L-valine (Val), L-leucine (Leu), L-isoleucine (Ile), L-phenylalanine (Phe), L-tryptophan (Trp), L-aspartic acid (Asp), L-glutamic acid (Glu), L-asparagine (Asn), L-glutamine (Glu), L-histidine (His), L-asparagine monohydrate (Arg), L-tyrosine (Tyr), and L-ascorbic acid were purchased from FUJIFILM Wako (Osaka, Japan). Acetic acid (AA), L-lysine (Lys), and sodium butyrate were purchased from Nacalai Tesque (Kyoto, Japan), and proline (Pro) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were of analytical grade.

Takakuwa A., Nakamura K., Kikuchi M., Sugimoto R., Ohira S., Yokoi Y, & Ayabe T. (2019). Butyric Acid and Leucine Induce α-Defensin Secretion from Small Intestinal Paneth Cells. Nutrients, 11(11), 2817.

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Formulation and Characterization of VD3

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Materials used in this study were purchased from the following commercial vendors: VD3 from Cayman Chemical (Ann Arbor, MI, USA). Excipients (special grade)—L-alanine (Ala), L-methionine (Met), L-phenylalanine (Phe), L-leucine (Leu), L-valine (Val) and L-isoleucine (Ile)—and reagents—ethanol (99.5%) (reagent grade), acetonitrile (HPLC grade) and polyoxyethylene (20) sorbitan monolaurate (biochemistry grade)—from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). 2 mL VIST glass vials obtained from Daiwa Special Glass (Osaka, Japan) and rubber stoppers (F5-43) obtained from Sumitomo Rubber Industries (Hyogo, Japan) were used as the packing materials.

Miyamoto K., Yanagisawa M., Taga H., Yamaji H., Akita T, & Yamashita C. (2021). Optimization of Very Low-Dose Formulation of Vitamin D3 with Lyophilizate for Dry Powder Inhalation System by Simple Method Based on Time-of-Flight Theory. Pharmaceutics, 13(5), 632.

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Amino Acid Reagent Preparation

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MEM essential amino acids solution, MEM non-essential amino acids solution, l-cystine, l-arginine, l-histidine, l-Isoleucine, l-leucine, l-lysine, l-methionine, l-phenylalanine, l-threonine, l-tryptophan, l-tyrosine, l-valine, William's Medium E (WME), Dulbecco's modified Eagle's medium (DMEM), and hanks' balanced salt solution (HBSS), were purchased from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Proteinase inhibitor cocktail was purchased from Nacalai Tesque, (Kyoto, Japan). Na₂S₄ was purchased from Dojindo Molecular Technologies, Inc (Kumamoto, Japan). β-(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM) was purchased from Molecular Biosciences (CO, USA). All other reagents and chemicals were of the highest grades available.

Akiyama M., Unoki T., Aoki H., Nishimura A., Shinkai Y., Warabi E., Nishiyama K., Furumoto Y., Anzai N., Akaike T., Nishida M, & Kumagai Y. (2022). Cystine-dependent antiporters buffer against excess intracellular reactive sulfur species-induced stress. Redox Biology, 57, 102514.

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Neurotransmitter Precursor Compounds

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Phenylethylamine hydrochloride (Cat# P0086), tryptamine (Cat# T0890), tyramine hydrochloride (Cat# A0303), L-tyrosine (Cat# T0550), carbidopa monohydrate (Cat# C2450), methyldopa sesquihydrate (Cat# D1817), and benserazide hydrochloride (Cat# B4108) were purchased from Tokyo Chemical Industry (Tokyo, Japan). L-Phenylalanine (Cat# 169–01303), Amino Acids Mixture Standard Solution, Type H (Cat# 013–08391) were obtained from FUJIFILM Wako Pure Chemical (Osaka, Japan). L-Tryptophan (Cat# 35607–74) was obtained from Nacalai Tesque (Kyoto, Japan). Other reagents of analytical grade were from FUJIFILM Wako Pure Chemical, Nacalai Tesque (Kyoto, Japan), and Sigma-Aldrich (St. Louis, MO, USA).

Sugiyama Y., Mori Y., Nara M., Kotani Y., Nagai E., Kawada H., Kitamura M., Hirano R., Shimokawa H., Nakagawa A., Minami H., Gotoh A., Sakanaka M., Iida N., Koyanagi T., Katayama T., Okamoto S, & Kurihara S. (2022). Gut bacterial aromatic amine production: aromatic amino acid decarboxylase and its effects on peripheral serotonin production. Gut Microbes, 14(1), 2128605.

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