Antibody against human mouse abca1

Manufactured by Thermo Fisher Scientific

The Antibody against human/mouse ABCA1 is a lab equipment product that can be used to detect the presence and quantify the levels of the ABCA1 protein in human and mouse samples. ABCA1 is a membrane-bound transporter protein involved in the regulation of cellular cholesterol and phospholipid homeostasis. This antibody can be used in various laboratory techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and distribution of ABCA1 in different biological systems.

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Lab products found in correlation

Enhanced chemiluminescence, by Thermo Fisher Scientific (2 mentions) Hrp conjugated secondary antibodies against rabbit and mouse igg, by Bio-Rad (2 mentions)

Spelling variants of this product

ABCA1 (6 citations)

2 protocols using antibody against human mouse abca1

Western Blot Analysis of Cholesterol Homeostasis

Cited 1 time

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Western blots were performed as described previously [40 (link)]. Antibodies against FDXR, p21, mevalonate decarboxylase (MVD), mevalonate kinase (MVK), and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody against human/mouse ABCA1 was purchased from Invitrogen Life Technologies (Carlsbad, CA) and Cell signaling Technology (#96292, Danvers, MA). Antibodies against SREBP1 and SREBP2 were purchased from Abcam (Cambridge, MA). HRP-conjugated secondary Antibodies against rabbit and mouse IgG were purchased from BioRad (Hercules, CA). The immunoreactive bands were visualized by enhanced chemiluminescence (Thermo Fisher Scientific Inc, Carlsbad, CA) and photographed with the BioSpectrum^® 810 Imaging System (UVP LLC, Upland, CA).

Zhang Y., Mohibi S., Vasilatis D.M., Chen M., Zhang J, & Chen X. (2022). Ferredoxin reductase and p53 are necessary for lipid homeostasis and tumor suppression through the ABCA1–SREBP pathway. Oncogene, 41(12), 1718-1726.

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Western Blot Analysis of Metabolic Regulators

Cited 1 time

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Western blots were performed as described previously (40 (link)). Antibodies against FDXR, p21, mevalonate decarboxylase (MVD), mevalonate kinase (MVK) and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody against human/mouse ABCA1 was purchased from Invitrogen Life Technologies (Carlsbad, CA) and Cell signaling technology (#96292, Danvers, MA). Antibodies against SREBP1 and SREBP2 were purchased from Abcam (Cambridge, MA). HRP-conjugated secondary Antibodies against rabbit and mouse IgG were purchased from BioRad (Hercules, CA). The immunoreactive bands were visualized by enhanced chemiluminescence (Thermo Fisher Scientific Inc, Carlsbad, CA) and photographed with the BioSpectrum® 810 Imaging System (UVP LLC, Upland, CA).

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