Cell-free culture fluids were prepared by back-diluting V. cholerae overnight cultures 1:1,000 in the relevant media and incubating at 37 °C with shaking for 7–12 h. Cultures were subjected to centrifugation at 4,000 rpm for 10 min at 4 °C and culture fluids were filtered through 0.22 µm vacuum driven syringe filters (Millipore).
0.22 m vacuum driven syringe filters
Manufactured by Merck Group
The 0.22 µm vacuum driven syringe filters are a type of laboratory equipment used for filtration. They are designed to remove particulates and microorganisms from liquid samples. The filters have a pore size of 0.22 micrometers and are driven by vacuum pressure to facilitate the filtration process.
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Lab products found in correlation
2 protocols using 0.22 m vacuum driven syringe filters
1
Preparing Cell-free Vibrio cholerae Cultures
2
Preparing Cell-free Vibrio cholerae Cultures
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Cell-free culture fluids were prepared by back-diluting V. cholerae overnight cultures 1:1,000 in the relevant media and incubating at 37 °C with shaking for 7–12 h. Cultures were subjected to centrifugation at 4,000 rpm for 10 min at 4 °C and culture fluids were filtered through 0.22 µm vacuum driven syringe filters (Millipore).
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