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Orca er camera

Manufactured by Zeiss
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Sourced in Japan

The ORCA-ER camera is a high-performance scientific imaging camera designed for a wide range of microscopy applications. It features a large, high-resolution CCD sensor with low noise and high quantum efficiency, providing excellent image quality and sensitivity. The camera's fast readout speeds and flexible binning capabilities make it well-suited for a variety of live-cell and low-light imaging experiments.

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Lab products found in correlation

Axiovert 200m, by Zeiss (4 mentions) Apotome 2, by Zeiss (4 mentions) Axio observer 7, by Zeiss (3 mentions) Zen software, by Zeiss (3 mentions) Lsm 700, by Zeiss (2 mentions) Alexa fluor 568 conjugated phalloidin, by Thermo Fisher Scientific (2 mentions) Axiovision software, by Zeiss (2 mentions) Observer z1m inverted microscope, by Hamamatsu Photonics (2 mentions) Axioobserver z1 epifluorescence microscope, by Hamamatsu Photonics (2 mentions) Illustrator cc 2015, by Adobe (2 mentions) Axiooberver z1 epifluorescence microscope, by Hamamatsu Photonics (2 mentions) Lsm 710 confocal system, by Zeiss (2 mentions) Lsm zen software, by Zeiss (2 mentions) Observer z1 fluorescent microscope, by Zeiss (2 mentions) Improvision volocity software, by PerkinElmer (2 mentions) Axio observer z1, by Zeiss (1 mentions) Hoechst 33342 trihydrochloride trihydrate, by Thermo Fisher Scientific (1 mentions) Observer z1, by Zeiss (1 mentions) Axiocam mrm camera, by Zeiss (1 mentions) Observer z 1, by Hamamatsu Photonics (1 mentions)

Spelling variants of this product

Orca ER (8 citations) ORCA-ER digital camera (5 citations) ORCA-ER CCD camera (5 citations) ORCA ER camera and apotome (2 citations) ORCA-ER digital camera C4742-95 (2 citations) Orca-ER 1394 C4742-80 camera (2 citations)

16 protocols using orca er camera

1

Multimodal Imaging of Cytoskeleton and Organelles

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Confocal fluorescence imaging was performed with a Zeiss LSM700 laser scanning confocal microscope on a Zeiss Axio Observer Z1 inverted stand using a long working distance water immersion C-Apochromat 40×/1.1 NA objective operated by Zen software (version 2010). For filamentous actin imaging, siRNA-treated cells seeded in microtracks were fixed, permeabilized, blocked as previously described8 (link) and stained with Alexa Fluor 568-conjugated phalloidin (Life Technologies) overnight. For nuclei and Golgi apparatus imaging, live cells in microtracks were incubated in complete media for 5 minutes with a 1:1000 dilution of Hoechst 33342, trihydrochloride trihydrate (Invitrogen) and incubated overnight with Golgi-RFP (Cell Light®, BacMam 2.0, Life Technologies) respectively. Live-cell time-lapse confocal fluorescence imaging of the nucleus and Golgi apparatus was performed in temperature-, humidity- and CO2-controlled incubation chambers. Live cell phase contrast time-lapse images of cells were obtained using a Zeiss observer Z1m inverted microscope equipped with a Hamamatsu ORCA-ER camera operated by AxioVision software (version 4.8.1.0, Carl Zeiss Microscopy, Thornwood, NY).
Rahman-Zaman A., Shan S, & Reinhart-King C.A. (2017). Cell Migration in Microfabricated 3D Collagen Microtracks is Mediated Through the Prometastatic Protein Girdin. Cellular and Molecular Bioengineering, 11(1), 1-10.
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2

Multimodal Microscopy Imaging Protocol

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The following systems were used: for confocal imaging, Zeiss LSM880-META NLO confocal microscope equipped with Airyscan; for color imaging, Zeiss Observer Z1 with a Hamamatsu ORCA-ER camera; for fluorescence imaging, Zeiss Observer Z1 with an AxioCam MRm camera. All histology analysis and immune-fluorescent staining analysis were quantitated in a blinded way by at least two investigators.
Miao L., Li J., Li J., Tian X., Lu Y., Hu S., Shieh D., Kanai R., Zhou B.Y., Zhou B., Liu J., Firulli A.B., Martin J.F., Singer H., Zhou B., Xin H, & Wu M. (2018). Notch signaling regulates Hey2 expression in a spatiotemporal dependent manner during cardiac morphogenesis and trabecular specification. Scientific Reports, 8, 2678.
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3

Fluorescence and Brightfield Time-Lapse Imaging

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For fluorescence time-lapse imaging, cells were imaged in 24-well plates in DMEM (no phenol red) with a heated 37°C chamber in 5% CO2. Images were taken every 4 min with a 20×/0.4 NA air objective using a Zeiss Axio Observer 7 with a CMOS Orca flash 4.0 camera at 4 × 4 binning. For brightfield imaging, cells were imaged in a 24-well plate in DMEM in a heated chamber (37°C and 5% CO2) with a 10×/0.5 NA air objective using a Hamamatsu ORCA-ER camera at 2 × 2 binning on a Zeiss Axiovert 200M, controlled by Micro-manager software (open source; https://micro-manager.org/) or with a 20×/0.4 NA air objective using a Zeiss Axio Observer 7 as detailed above. Mitotic exit was defined by cells flattening down in the presence of nocodazole and MPS1 inhibitor. In double mutant assays where both recombinant BUBR1 and BUB1 are expressed (Figs. 2 H and S3 I), cells were selected for quantification based on high levels of YFP as an indication of successful transient transfection of BUBR1 constructs.
Cordeiro M.H., Smith R.J, & Saurin A.T. (2020). Kinetochore phosphatases suppress autonomous Polo-like kinase 1 activity to control the mitotic checkpoint. The Journal of Cell Biology, 219(12), e202002020.
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4

Multi-Modal Imaging of Tissue Samples

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Tissue sections, primary cells and organoid samples were imaged with the 20x objective of an AxioObserver.Z1 epifluorescence microscope equipped with a Hamamatsu ORCA-ER camera and ApoTome.2 (Carl Zeiss) slider. Additionally, organoid samples were imaged at 20x on an inverted LSM 780 laser scanning confocal microscope (Zeiss) or an inverted spinning disk confocal system (Andor Technology Inc). The LSM 780 confocal microscope is equipped with an inverted Zeiss Axio Observer Z1 microscope with definite focus, 405, 440, 488, 514, 561, 594 and 633 laser lines. The system was driven by the Zen software (Zeiss). Cleared whole-mount tissue specimens were exclusively imaged using the 20x objective of an inverted Dragonfly 202 spinning disk confocal system (Andor Technology Inc) equipped with a 40 μm pinhole and a Leica DMi8 camera with AFC (20x air objective). Four laser lines (405, 488, 561 and 625 nm) were used for near simultaneous excitation of DAPI, Alexa-448, RRX and Alexa-647 fluorophores. The system was driven by the Andor Fusion software. For the majority of experiments z-stack images of 1-3 μm steps were collected. For each experiment images were acquired at identical settings.
Niec R.E., Chu T., Schernthanner M., Gur-Cohen S., Hidalgo L., Pasolli H.A., Luckett K.A., Wang Z., Bhalla S.R., Cambuli F., Kataru R.P., Ganesh K., Mehrara B.J., Pe’er D, & Fuchs E. (2022). Lymphatics act as a signaling hub to regulate intestinal stem cell activity. Cell stem cell, 29(7), 1067-1082.e18.
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5

Epifluorescence Microscopy for Z-Stack Imaging

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Images were taken with an AxioObserver.Z1 epifluorescence microscope equipped with a Hamamatsu ORCA-ER camera and ApoTome.2 (Carl Zeiss) slider. Images were collected using either a 20X air or 40X oil objective controlled by Zen software (Carl Zeiss). Maximal projection Z-stacks are presented. All image processing was done with ImageJ software. RGB images were assembled in Adobe Illustrator 2021.
Larsen S.B., Cowley C.J., Sajjath S.M., Barrows D., Yang Y., Carroll T.S, & Fuchs E. (2021). Establishment, Maintenance and Recall of Inflammatory Memory. Cell stem cell, 28(10), 1758-1774.e8.
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6

Quantifying ELT-2 Protein Levels in Embryos

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ELT-2 protein levels in embryos were measured as described previously (Van Fürden et al. 2004 (link); Wiesenfahrt et al. 2016 (link)), using the anti-ELT-2 monoclonal antibody 455-2A4. Images were recorded with a Hamamatsu Orca ER camera attached to a Zeiss Axioplan 2i microscope (40X objective); images for comparing different strains were collected with constant microscope/camera parameters. Total fluorescent intensity in the intestinal primordium (2E or 8E cell stage embryo) was measured (in arbitrary units) using ImageJ and corrected for background intensity measured elsewhere in the embryo. The probability that ELT-2 levels measured in one set of embryos have the same distribution of intensities as measured in JM247 embryos (single copy end-1p::elt-2 transgene) was calculated by the Wilcoxon-Mann-Whitney rank test (Marx et al. 2016 (link)) implemented online (https://ccb-compute2.cs.uni-saarland.de/wtest/).
Wiesenfahrt T., Duanmu J., Snider F., Moerman D., Au V., Li-Leger E., Flibotte S., Parker D.M., Marshall C.J., Nishimura E.O., Mains P.E, & McGhee J.D. (2018). A Strategy To Isolate Modifiers of Caenorhabditis elegans Lethal Mutations: Investigating the Endoderm Specifying Ability of the Intestinal Differentiation GATA Factor ELT-2. G3: Genes|Genomes|Genetics, 8(5), 1425-1437.
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7

Cell Viability and Transfection Assay

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After the flow experiments, cells were stained with 1 μg/ml propidium iodide to identify dead cells and with 2 μg/ml Hoechst 33342 to determine the total number of cells. Following staining, cells were fixed with 4% paraformaldehyde (PFA). Cells were visualized under Leica DM IL LED–DFC295 or Leica DM IL LED–DFC290 inverted phase contrast microscopes. Cells were imaged using a Hamamatsu ORCA-ER camera coupled to a Zeiss Axiovert 200 inverted fluorescent microscope with a fully motorised stage, controlled by Improvision Volocity acquisition software. To count cells and determine transfection efficiencies as well as cell viabilities, the acquired microscopy images were processed in ImageJ 1.4734 (link)35 (link). See SI Text for further details on materials and methods used.
Kis Z., Rodin T., Zafar A., Lai Z., Freke G., Fleck O., Del Rio Hernandez A., Towhidi L., Pedrigi R.M., Homma T, & Krams R. (2016). Development of a synthetic gene network to modulate gene expression by mechanical forces. Scientific Reports, 6, 29643.
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8

Epifluorescence Microscopy for Tissue Imaging

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Images were acquired with an AxioOberver.Z1 epifluorescence microscope equipped with a Hamamatsu ORCA-ER camera and an ApoTome.2 (Carl Zeiss) slider. Tiled and stitched images of sagittal sections were collected using a 20X or 40X objective, controlled by Zen software (Carl Zeiss). Maximal projection Z-stacks are presented and co-localizations were interpreted only in single Z-stacks. Z-stacks were projected using ImageJ software. RGB images were assembled in Adobe Illustrator CC2015.3.
Naik S., Larsen S.B., Gomez N.C., Alaverdyan K., Sendoel A., Yuan S., Polak L., Kulukian A., Chai S, & Fuchs E. (2017). Inflammatory Memory Sensitizes Skin Epithelial Stem Cells to Tissue Damage. Nature, 550(7677), 475-480.
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9

Epifluorescence Microscopy for Tissue Imaging

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Images were acquired with an AxioOberver.Z1 epifluorescence microscope equipped with a Hamamatsu ORCA-ER camera and an ApoTome.2 (Carl Zeiss) slider. Tiled and stitched images of sagittal sections were collected using a 20X or 40X objective, controlled by Zen software (Carl Zeiss). Maximal projection Z-stacks are presented and co-localizations were interpreted only in single Z-stacks. Z-stacks were projected using ImageJ software. RGB images were assembled in Adobe Illustrator CC2015.3.
Naik S., Larsen S.B., Gomez N.C., Alaverdyan K., Sendoel A., Yuan S., Polak L., Kulukian A., Chai S, & Fuchs E. (2017). Inflammatory Memory Sensitizes Skin Epithelial Stem Cells to Tissue Damage. Nature, 550(7677), 475-480.
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10

Fluorescence and Brightfield Imaging of Mitotic Exit

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For fluorescence imaging, cells were imaged in 8‐well chamber slides (ibidi), on either a Zeiss Axio Observer 7 with a CMOS Orca flash 4.0 camera or a DeltaVision Elite equipped with Photometrics CascadeII:1024 EMCCD or CoolSNAP HQ (Photometrics) camera. The objectives used for fluorescent imaging were either 20×/0.8 NA or 40×/1.3NA. For brightfield imaging, cells were imaged in a 24‐well plate in DMEM on a Zeiss Axiovert 200M using Hamamatsu ORCA‐ER camera and controlled by Micro‐manager software (open source: http://micro-manager.org), or a Zeiss Axio Observer 7 as detailed above. The air objectives used for brightfield imaging were either 10×/0.5 NA or a 20×/0.4 NA. Mitotic exit was defined by cells flattening down in the presence of nocodazole and MPS1 inhibitor.
Allan L.A., Camacho Reis M., Ciossani G., Huis in ‘t Veld P.J., Wohlgemuth S., Kops G.J., Musacchio A, & Saurin A.T. (2020). Cyclin B1 scaffolds MAD1 at the kinetochore corona to activate the mitotic checkpoint. The EMBO Journal, 39(12), e103180.
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