Anti-CD68 (PG-M1); anti-HepPar 1 (OCH1E5) (Dako, Denmark); anti-ApoA1 (EP1368Y) (Acris Antibodies, Germany); and anti-LAMP1 (H4A3, University of Iowa, Department of Biology, Iowa City, IA) were murine and rabbit monoclonal antibodies. Anti-SR-B1 (Novus Biologicals, Littleton), anti-ApoB (Meridian Life Science, USA), anti-Rab11 (Abcam, Cambridge, UK), and anti-HBs (gift from Heinz Schaller, Heidelberg) were rabbit polyclonal antibodies. LDL-R blocking antibody was from Acris Antibodies, Germany. Secondary antibodies were goat Alexa Flour 488, 594, 647 conjugates to mouse and rabbit immunoglobulin G (Invitrogen, Germany). Other reagents were BODIPY493/503, Alexa Fluor 594 carboxylic acid, succinimidyl ester, NBD-cholesterol (Invitrogen, Germany), 32P (Hartmann Analytic, Germany), purified ApoA1, HDL (Acris Antibodies, Germany), filipin, Fluorescein-5-Isothiocyanat (FITC), PFA (Sigma-Aldrich, Germany), and DAPI (Roche, Germany). Remaining chemicals were from Carl Roth (Germany).
Anti rab11
Manufactured by Abcam
Sourced in United States, United Kingdom
Anti-Rab11 is a primary antibody that targets the Rab11 protein. Rab11 is a member of the Ras superfamily of small GTPases and is involved in the regulation of vesicle trafficking and recycling endosomes. The Anti-Rab11 antibody can be used to detect and study the Rab11 protein in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti rab5, by Abcam (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions)
Anti sr b1, by Novus Biologicals (1 mentions)
Alexa fluor 594 carboxylic acid, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Bodipy 493 503, by Thermo Fisher Scientific (1 mentions)
Nbd cholesterol, by Thermo Fisher Scientific (1 mentions)
Filipin, by Merck Group (1 mentions)
Anti alix antibody, by Abcam (1 mentions)
Anti rab27a, by Abcam (1 mentions)
Anti cd9 antibody, by Abcam (1 mentions)
Anti rab27b, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti cd63 antibody, by Abcam (1 mentions)
Skim milk, by Merck Group (1 mentions)
Electrophoresis apparatus, by Bio-Rad (1 mentions)
Anti β actin antibody, by Abcam (1 mentions)
Anti app, by Merck Group (1 mentions)
Anti bace1, by Merck Group (1 mentions)
Anti nicastrin, by Cell Signaling Technology (1 mentions)
6 protocols using anti rab11
1
Immunofluorescence Microscopy of Lipid Trafficking
2
Western Blot Analysis of Exosomal Markers
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After quantitative analysis, the total protein of the sample was extracted and denatured in this study. SDS–PAGE gel was used for electrophoresis, electrophoresis apparatus (Bio‐RAD) was adjusted to 120 V for electrophoresis, PVDF membrane (Millipore) was used for membrane transfer, and skim milk (Sigma) was used for blocking. Primary antibodies (Abcam) were then added and incubated overnight. The antibodies used were as follows: anti‐Rab11 (Abcam, 1:1000), anti‐Rab27a (Abcam, 1:200), anti‐Rab27b (Abcam, l:500), anti‐TSAP6 antibody (Abcam, 1:1000), anti‐CD63 antibody (Abcam, 1:1000), anti‐CD9 antibody (Abcam, 1:1000), anti‐Alix antibody (Abcam, 1:1000), and anti‐β‐Actin antibody (Abcam, 1:10,000). The membrane was then incubated with the primary antibody diluted in advance. Subsequently, the membranes were incubated with IgG (Abcam, 1:300) for 1 h. Finally, the protein bands were visualized using a Pierce ECL Western blotting kit. Protein expression was quantified using ImageJ to analyze band gray values. β‐Actin was used as a control.
3
Comprehensive Antibody Validation for Alzheimer's Research
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Primary antibodies included anti-Aβ 4G8 (SIG-39220, BioLegend, San Diego, CA, USA), anti-SPIN90 (generated in our laboratory), anti-Rab11 (ab3612, Abcam, Cambridge, UK), anti-GFP (sc-9996, Santa Cruz Biotechnology, Dallas, TX, USA), anti-APP (MAB348, EMD Millipore, Darmstadt, Germany), anti-BACE1 (B0681, Sigma, St. Louis, MO, USA), anti-Nicastrin (5665, Cell Signaling Technology, Danvers, MA, USA), anti-β-actin (sc-47778, Santa Cruz Biotechnology, Dallas, TX, USA), anti-p35/p25 (2680, Cell Signaling Technology, MA, USA) and anti-tubulin (T6199, Sigma, St. Louis, MO, USA). Secondary antibodies included horseradish peroxidase (HRP)-conjugated donkey anti-mouse (115-035-006) and anti-rabbit antibodies (111-035-006, Jackson Laboratory, Bar Harbor, ME, USA). Unless otherwise noted, all chemicals were purchased from Sigma.
4
Immunofluorescence and ADP-ribosylation Assay
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In this study anti-Rab7 (Sigma), anti-Rab11, and anti-Hsp90 (Abcam), anti-Actin (Santa Cruz), anti-FA (7F2) (Abcam), and anti-EEA1 (Abcam) were used as primary antibodies. Secondary antibody, and fluorescent (TRITC) labeled phalloidin were purchased from Santa Cruz, and Sigma, culture materials from Nunc, and Falcon firms, DTx, and CRM197 from Calbiochem, and ProLong Gold Antifade Mountant Invitrogen firms. For ADP-ribosylation assay [Adenosine-14C]NAD (NEN-Dupont) with a specific activity of 535 Ci/mol was used.
5
Detecting HIV-1 and Protein Knockdown
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HIV-1 p24 was detected using anti-HIV-1 core antigen antibody-FITC (KC57-FITC; Beckman Coulter), and actin labeled with Cytopainter Phallodin-iFluor-555 (Abcam). Protein knockdown was detected by immunoblotting using rabbit anti-ARF1, anti-BIN1, anti-RAB8A, mouse anti-Rab7L1 (Abcam), and actin (Merck) antibodies, followed by secondary HRP-conjugated goat anti-rabbit and anti-mouse antibodies (Dako). Confocal microscopy was carried out using the primary antibodies anti-human CD81-APC (BD), anti-EEA1, anti-CHMP2B, anti-LAMP1, anti-Rab7, anti-Rab11, anti-Rab5 (Abcam). HRP uptake was detected using anti-HRP (Jackson Immunolaboratory). All unlabeled primary antibodies were detected with secondary anti-rabbit Alexa Fluor 546 (Life Technologies). The pharmacological inhibitors LY294002, bafilomycin A1, and indinivir (Sigma-Aldrich) were used at 50 μM, 0.5 μM, and 2 μg/ml, respectively.
6
Immunohistochemical Analysis of Olfactory Receptors
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The following primary mouse antibodies were used: anti-GFP (1:100), anti-Bruchpilot (1:50, nc82, supernatant, Developmental Studies Hybridoma Bank [DSHB] ), and anti Rab11 (1:100, BD Biosciences). The primary rabbit antibodies, anti-GFP (1:2,000, TP-401, Torrey Pines), Lamp1, anti-Rab5, anti-Rab11 (1:100, Abcam), anti-Or22a (1:10,000) and anti-Orco (1:10,000) were gifts from Richard Benton. The secondary antibodies were conjugated to Alexa 488 or Alexa 568 (1:500, Molecular Probes). Antenna immunohistochemistry was performed as described previously (Couto et al., ). Or22a images were captured from a subset of stereotypic Or22a sensilla opposite to the arista. The confocal microscopy images were collected on an LSM 700 (Zeiss) and analyzed on a Zen image browser.
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