The normal human dermal fibroblast (NHDF) cell line was purchased from Lonza (Basel, Switzerland) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Cells were grown at 37 °C in an atmosphere containing 5% CO2 and passaged by trypsinization with 0.5% trypsin/ethylenediaminetetraacetic (EDTA).
Nhdf cell line
Manufactured by Lonza
The NHDF cell line is a primary human dermal fibroblast cell line derived from normal human skin. These cells are used in research and development applications to study cellular processes, drug testing, and tissue engineering.
Automatically generated - may contain errors
Lab products found in correlation
Antimycotics, by Thermo Fisher Scientific (2 mentions)
Glutamax supplement, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Dmem 4.5 g l glucose, by Lonza (1 mentions)
Horse serum, by Merck Group (1 mentions)
Dmem with high glucose, by Lonza (1 mentions)
Hepg2, by Thermo Fisher Scientific (1 mentions)
Hek293, by Thermo Fisher Scientific (1 mentions)
Egm 2, by Lonza (1 mentions)
4 protocols using nhdf cell line
1
Culturing Normal Human Dermal Fibroblasts
2
Cell Culture Protocol for HEK 293, HeLa, and NHDF
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HEK 293 cells were cultured in Dulbecco’s modified Eagle medium with high glucose (DMEM 4.5 g/l glucose, Lonza). The HeLa and NHDF cells were cultured in minimum essential medium alpha (MEMα, Lonza). All media were supplemented with 10 % fetal bovine serum (Sigma), GlutaMAX supplement (Gibco), antibiotics, and antimycotics (Gibco). The HEK 293 and HeLa cell lines were purchased from the Cell Line Collection of the Polish Academy of Sciences, Institute of Immunology and Experimental Therapy in Wroclaw. The NHDF cell line was purchased from Lonza.
3
Cultivation and Differentiation of Cell Lines
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The HEK293, murine myoblast C2C12, and rat cardiomyocyte H9C2 cell lines were cultured in DMEM with high glucose (4.5 g/L glucose; Lonza). HeLa cervix carcinoma cells and NHDF cells were cultured in minimum essential medium with alpha modification (MEMα; Lonza). Hepatocellular carcinoma HepG2 cells were cultured in Eagle’s minimum essential medium (EMEM; Lonza). HDMECs were cultured in endothelial cell growth medium (EGM-2, Lonza). All media were supplemented with 10% FBS (Sigma), GlutaMAX supplement, antibiotics, and antimycotics (Gibco).
The HEK293, HepG2, and HeLa cell lines were purchased from the Cell Line Collection of the Polish Academy of Sciences, which was available through the Institute of Immunology and Experimental Therapy in Wroclaw, Poland. The NHDF cell line was purchased from Lonza. The C2C12 cell line was obtained from the Cell Pathology Department of the University of Wroclaw. The H9C2 cell line was obtained from the Department of Medical Biochemistry of Wroclaw Medical University. The HDMEC cell line was purchased from PromoCell.
To obtain differentiated myotubes, C2C12 cells were seeded to reach 70%–80% confluence, and then a differentiation medium containing 2% horse serum (Sigma) instead of 10% FBS was applied and changed every 2–3 days. Myotubes were analyzed 6–7 days later (Figure S5 ).
The HEK293, HepG2, and HeLa cell lines were purchased from the Cell Line Collection of the Polish Academy of Sciences, which was available through the Institute of Immunology and Experimental Therapy in Wroclaw, Poland. The NHDF cell line was purchased from Lonza. The C2C12 cell line was obtained from the Cell Pathology Department of the University of Wroclaw. The H9C2 cell line was obtained from the Department of Medical Biochemistry of Wroclaw Medical University. The HDMEC cell line was purchased from PromoCell.
To obtain differentiated myotubes, C2C12 cells were seeded to reach 70%–80% confluence, and then a differentiation medium containing 2% horse serum (Sigma) instead of 10% FBS was applied and changed every 2–3 days. Myotubes were analyzed 6–7 days later (
4
Cytotoxicity Evaluation of Compounds in NHDF Cells
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Cell Line
The NHDF cell line (from LONZA) was used to determine the cytotoxicity effect of newly synthesized compounds. The cells were incubated in DMEM without phenol red supplemented with 10% fetal bovine serum (FBS), 2 mM L glutamine, 1.25 µg/mL amphotericin B, and 100 µg/mL gentamicin. The medium prepared in this way was stored at 4–8 °C. The NHDF cells were cultivated at 37 °C in 5% CO2 and 95% humidity. The cells were passaged, or the medium was changed twice a week.
The NHDF cell line (from LONZA) was used to determine the cytotoxicity effect of newly synthesized compounds. The cells were incubated in DMEM without phenol red supplemented with 10% fetal bovine serum (FBS), 2 mM L glutamine, 1.25 µg/mL amphotericin B, and 100 µg/mL gentamicin. The medium prepared in this way was stored at 4–8 °C. The NHDF cells were cultivated at 37 °C in 5% CO2 and 95% humidity. The cells were passaged, or the medium was changed twice a week.
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