The synthesis was performed as described previously for PlGF-2123–144 conjugates (16 (link)). Rat anti-mouse PD-L1 (clone: 10F.9G2, Bio X Cell) and hamster anti-mouse CTLA4 (clone: 9H10, Bio X Cell) were incubated with 15 eq. of sulfo-SMCC for 40 min at room temperature (RT). Excess sulfo-SMCC was removed using a Zeba spin desalting column (Thermo Fisher Scientific). >10 eq. of CBD protein (the VWF A3 domain, with an C-terminal cysteine residue) was then added and reacted for 1 hour at RT.
Rat anti mouse pd l1
Manufactured by BioXCell
Sourced in United States
The Rat anti-mouse PD-L1 is a laboratory reagent used in research applications. It is a monoclonal antibody that binds to the mouse programmed death-ligand 1 (PD-L1) protein. PD-L1 is a key regulator of the immune system and plays a role in immune responses.
Automatically generated - may contain errors
Lab products found in correlation
Zeba spin desalting column, by Thermo Fisher Scientific (1 mentions)
Rat igg2b, by BioXCell (1 mentions)
Syrian hamster igg, by BioXCell (1 mentions)
Mouse igg1 isotype control, by BioXCell (1 mentions)
3 methylcholanthrene mca, by Merck Group (1 mentions)
Rat igg2b isotype control, by BioXCell (1 mentions)
Rat igg1 isotype control, by BioXCell (1 mentions)
3 protocols using rat anti mouse pd l1
1
Synthesis of PD-L1/CTLA4 Conjugates
2
Modulating Tumor Immunity via CTLA4 and PD-L1
Check if the same lab product or an alternative is used in the 5 most similar protocols
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8- to 12-week-old female mice were injected subcutaneously with 100μl PBS containing 5×105 B16ova melanoma cells into the left, ventral flank. Intraperitoneal injection of 200μl PBS containing either 0.4mg of a Syrian hamster anti-mouse CTLA4 (Clone UC10-4F10-11; BE0131), 0.5mg rat anti-mouse PD-L1 (Clone10F.9G2; BE0101), 0.4mg Syrian hamster IgG (BE0087) or 0.5mg rat IgG2b (Clone LTF-2; BE0090) (all from BioXCell, USA) was performed every 3 days starting from day 0 of B16ova challenge until day 18.
Tumor volume was calculated according to the following equation: V = π/6*length*width2. For survival analysis, mice with tumors exceeding the length limit of 15 mm were sacrificed and counted as dead.
Tumor volume was calculated according to the following equation: V = π/6*length*width2. For survival analysis, mice with tumors exceeding the length limit of 15 mm were sacrificed and counted as dead.
3
Characterization of Tumor Immune Infiltrates
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