Both plasma and 24-h urine samples were collected post MC-LR exposure. Blood was collected by cardiac puncture in K3-EDTA microtubes (Sarstedt, Newton, NC, USA) and separated plasma was stored at −80 °C until further use. MC-LR in urine samples was quantified using both Ultra High Pressure Liquid Chromatography–triple Quadrupole–tandem Mass Spectrometry (UHPLC-QqQ-MS/MS, Columbia, MD, USA) and High Pressure Liquid Chromatography (Shimadzu Technologies, Addison, IL USA)–Orbitrap Fusion Mass Spectrometry (Thermo Fisher Scientific, San Jose, CA, USA) system, while plasma samples were analyzed using HPLC-Orbitrap Fusion MS as we have previously described [43 (link)].
K3 edta micro tubes
Manufactured by Sarstedt
Sourced in Germany
The K3 EDTA micro tubes are small containers designed for sample collection and storage. They are coated with the anticoagulant K3 EDTA, which helps to prevent blood samples from clotting.
Automatically generated - may contain errors
4 protocols using k3 edta micro tubes
1
Quantification of MC-LR in Plasma and Urine
2
Docetaxel vs. TH1902 neutrophil effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Athymic nude mice were treated with docetaxel or TH1902 at a docetaxel‐equivalent i.v. dose (15 mg/kg). Blood samples were collected in K3 EDTA micro tubes (Sarstedt) 4 days before the start of treatment, 4 days after the first and third treatments at docetaxel MTD, and 4 days after the first, third, and sixth treatments of TH1902. Blood neutrophil counts were performed on the day they were collected at the Diagnostic Laboratory of the Comparative Medicine Animal Resources Centre at McGill University.
3
Plasma Cytokine Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
While under isoflurane anesthesia, blood was collected from each mouse into K3 EDTA micro tubes (Sarstedt, Nümbrecht, Germany). Each blood sample was centrifuged at 3000× g for 10 min at 4 °C to obtain the plasma. The levels of plasma cytokines were measured using a mouse cytokine/chemokine discovery assay (Eve Technologies, Calgary, AB, Canada) [15 (link)].
4
Cytokine Profiling in Mouse Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After fully isoflurane anesthesia, retroorbital blood was collected in K3EDTA micro tubes (Sarstedt, Nümbrecht, Germany) and plasma separated by centrifugation (3,000 g for 10 min at 4°C). Plasma samples were kept at -80°C until analysis. The levels of plasma cytokines were determined by multiplex immunoassay with a BioPlex 200 Mouse Cytokine Array/Chemokine Array (Eve Technologies, Calgary, Canada) (18 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!