Fluorescein conjugate a sirna

The knockdown of ATG5 (Santa Cruz Biotechnology, sc-41445) was performed in BC3 and Raji cell lines using specific small interfering RNA duplex. The day before transfection, 3 × 10⁵ cells were seeded in 12-well culture plates in RPMI medium without antibiotics. Subsequently, 75 pmol of siRNA duplex and 7.5 µl of Lipofectamine 2000 Transfection Reagent (Life Technologies, 11668027) were diluted in Optimem medium (Life Technologies, 31985062) and added to the cells for 48 hrs. Then, cells were treated with bortezomib (BZ) at 20 nM for 24 hrs.
The transfection efficiency was evaluated by a Fluorescein Conjugate-A siRNA (Santa Cruz Biotechnology, sc-36869), which was also used as a scrambled control.

HSF1, NRF2 and SQSTM1 knockdown was performed in PEL cell lines using specific small interfering RNA. Subsequently, 3 × 10⁵ cells were seeded in 12-wells culture plate in RPMI medium supplemented with 10% fetal bovine serum (FBS) (Corning, NY, USA; 35-079), with L-glutamine and without antibiotics. Subsequently, 30 pmoli of siRNA duplex (siRNAHSF1, siRNANRF2 and siRNASQSTM1) (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-35611, sc-37030 and sc-29679) and 7 µL of Lipofectamine 2000 Transfection Reagent (Life Technologies, 11668027) were diluted in Optimem medium (Thermo Fisher Scientific, Waltham, MA, USA; 31985062) for 20 min at room temperature. The mixture was added to the cell culture for 48 h. After this, the cells were treated either with TPA (20 ng/mL) (T) and sodium butyrate (0.3 mM), or with bortezomib (20 nM) for the last 24 h.
Transfection efficiency was evaluated by Fluorescein Conjugate-A siRNA (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-36869). A control siRNA-A (siRNASC) (Santa Cruz Biotechnology Inc., Dallas, TX, USA; sc-37007) was also used [21 (link)].

The knockdown of Beclin 1 (Santa Cruz Biotechnology Inc., sc-29797) was performed in PEL cell lines using specific small interfering RNA. The day before transfection, 3×10⁵ cells were seeded in 12 well culture plate in RPMI medium without antibiotics. Subsequently, 75 pmoli of siRNA duplex and 7,5 μl of Lipofectamine 2000 Transfection Reagent (Life Technologies, 11668027) were diluted in Optimem medium (Life Technologies, 31985062) and added to the cells for 48 hrs. The transfection efficiency was evaluated by a Fluorescein Conjugate-A siRNA (Santa Cruz Biotechnology Inc., sc-36869), that it was also used as scrambled control.