Human TNBC cell line MDA-MB-231 was purchased from the cell bank of Institute of Zoology of the Chinese Academy of Sciences (Kunming, China) and MDA-MB-468 was purchased from Cobioer Biosciences Company (Nanjing, China). Cells were cultured in DMEM medium (Gibco) supplemented with 10% FBS (Gibco), 100 U/ml penicillin (Beyotime) and 0.1 mg/ml streptomycin (Beyotime) at 37°C with 5% CO2.
Mda mb 468
Manufactured by Cobioer Biosciences
Sourced in China
The MDA-MB-468 is a common cell line used in research, derived from a triple-negative breast cancer tumor. It is a well-established model system for studying various aspects of cancer biology, including cell proliferation, signaling pathways, and drug responses. The MDA-MB-468 cell line is widely used in the field of oncology research.
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Lab products found in correlation
Mda mb 231, by Cobioer Biosciences (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Beyotime (1 mentions)
Streptomycin, by Beyotime (1 mentions)
Mem medium, by Corning (1 mentions)
Mda mb 468, by Corning (1 mentions)
Hek293t, by Cobioer Biosciences (1 mentions)
Su dhl 6, by Corning (1 mentions)
Hs 578t, by Cobioer Biosciences (1 mentions)
Rpmi 1640 medium, by Corning (1 mentions)
Hek293t, by Corning (1 mentions)
Dmem medium, by Corning (1 mentions)
Mda mb 231, by Corning (1 mentions)
Ab6671, by Abcam (1 mentions)
Bt 474, by Cobioer Biosciences (1 mentions)
Mda mb 453, by Cobioer Biosciences (1 mentions)
Mda mb 453, by MedChemExpress (1 mentions)
Mda mb 231, by MedChemExpress (1 mentions)
Gdc 0941, by MedChemExpress (1 mentions)
6 protocols using mda mb 468
1
TNBC Cell Line Culturing Protocol
2
Cultivation of Human Cancer Cell Lines
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Human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and Hs578T), DLBCL cell line (Pfeiffer, Karpas 422, WSU-DLCL2 and SU-DHL6), and HEK293T were purchased from Cobioer Biosciences Co., Ltd (Nanjing, China). Pfeiffer, Karpas 422, WSU-DLCL2 and SU-DHL6 cell lines were cultured in RPMI-1640 medium (Corning, Midland, NY, USA) with 10% FBS (VivaCell, Shanghai, China), and MDA-MB-231, MDA-MB-468 and HEK293T were cultured in DMEM medium (Corning) with 10% FBS (VivaCell, Shanghai, China), Hs578T were cultured in MEM medium (Corning, Midland, NY, USA) with 10% FBS(VivaCell, Shanghai, China) and supplemented with 1% penicillin/streptomycin (v/v).
3
Investigating Breast Cancer Cell Lines
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Breast cancer cell lines BT-474, MCF7, Hs-578-T, MDA-MB-231, MDA-MB-453, and MDA-MB-468 (Cobioer, Nanjing, China) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Sunncell, Wuhan, China) with 10% fetal bovine serum (FBS) and certain cell growth factor. BT-549, HCC1937, and T47D cell lines were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Sunncell, Wuhan, China) with 10% or 20% FBS.
For the following function experiments, T47D, MCF7, MDA-MB-453, HCC1937, MDA-MB-231, and MDA-MB-468 cells were treated with 100 or 500 nM GDC-0941 (PI3K selective inhibitor, MedChemExpress, New Jersey, USA) or dimethyl sulfoxide (DMSO) for 24 h. In addition, MDA-MB-231 and HCC1937 cells were treated with 100 nM OSU-T315 (ILK inhibitor, SolelyBio, Beijing, China), tumor necrosis factor (TNF)-α, IgG, or TNF-α antibody (ab6671, Abcam, Cambridge, UK).
In addition, MCF7 and MDA-MB-453 cells were transfected with ILK, and MDA-MB-231 and HCC1937 cells were transfected with shILK.
For the following function experiments, T47D, MCF7, MDA-MB-453, HCC1937, MDA-MB-231, and MDA-MB-468 cells were treated with 100 or 500 nM GDC-0941 (PI3K selective inhibitor, MedChemExpress, New Jersey, USA) or dimethyl sulfoxide (DMSO) for 24 h. In addition, MDA-MB-231 and HCC1937 cells were treated with 100 nM OSU-T315 (ILK inhibitor, SolelyBio, Beijing, China), tumor necrosis factor (TNF)-α, IgG, or TNF-α antibody (ab6671, Abcam, Cambridge, UK).
In addition, MCF7 and MDA-MB-453 cells were transfected with ILK, and MDA-MB-231 and HCC1937 cells were transfected with shILK.
4
Western Blot Assay for Cell Lines
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Cell lines of HEK-293, PC-3, and MDA-MB-468 were all obtained from Nanjing Cobioer Biosciences (all originated from the American Type Culture Collection). The cells were cultured in DMEM-HG (HEK-293 and MDA-MB-468) and F-12K nutrient mixture (PC-3), supplemented with 10% fetal bovine serum. The cells were cultured in a humidified incubator (5% CO2, 37°C) and were passaged when the confluence reached 80%–90%. For the western blot assay, cells in logarithmic growth phase were inoculated into 6-well plates with 5 × 105 cells per well and cultured at 5% CO2 and 37°C for 24 h. On the next day, the cells in five wells were given different concentrations of the compound (1000 nM, 333.33 nM, 111.11 nM, 37.37 nM, and 12.35 nM), and the remaining well received an equal volume of DMSO (final concentration of DMSO in each well was 0.5%). The cells were collected 24 h later to extract total protein for western blot detection.
5
Curdione and Docetaxel Cytotoxicity on Breast Cancer Cells
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Breast cancer cell line MDA-MB-468 (Cobioer, Nanjing, China) was cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco; Thermo Fisher Scientific Inc., MA, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin-streptomycin (P/S, Solarbio Science & Technology Co., Ltd., Beijing) at 37°C in a humidified incubator with 5% CO2. Normal breast epithelial cell line MCF10A (Procell, Wuhan, China) was culture in DMEM/F12 (HyClone, Logan, UT, USA) with 10% FBS and 1% P/S. Cells were treated with different concentrations of Curdione and/or 1 µg/ml docetaxel (DTX, Sigma, MO, USA).
6
Xenograft Tumor Models of Breast Cancer
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The following cell lines were obtained from Cobioer (Cobioer Biosciences Co., Ltd., Nanjing, China): MDA-MB-231, T47D, MDA-MB-468, MDA-MB-157, MCF-7 and MCF-10A. Human breast cancer cell lines MDA-MB-231, T47D, MDA-MB-468, MDA-MB-157 and MCF-7 were preserved in RPMI 1640 supplemented with 10% FBS and were growth in a 37℃ humidi ed chamber with 5% CO 2 . MCF10A cells were cultured in Dulbecco's modi ed Eagle's medium/F12 with 10% horse serum, 20 ng/ml epidermal growth factor, 0.5 ug/ml hydrocortisone, 10 ug/ml insulin, 100 ng/ml cholera toxin. The passage number for each cell line was less than 15 when the experiments were performed. Female BALB/c nude mice, 6 to 8 weeks old, were purchased from Guangdong Medical Animal Experimental Center (Guangdong, China). Animal experimental procedures comply with the Care and Use of Laboratory Animals Guide and approved by the Animal Experimental Ethics Committee of Southern Medical University. MDA-MB-468, T47D and MCF-7 cells (1.0 × 10 7 in 0.2 ml PBS) were subcutaneously injected into the right side of the posterior anks of nude mice (n = 5), respectively. When tumor volumes reached 1000 mm 3 , mice were sacri ced by exposure to carbon dioxide and tumors were excised.
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