2 4 6 trichlorophenol

l-phenylalanine (F), glycine (G), l-Cysteine (C), l-Histidine (H) copper (II) chloride dihydrate (CuCl₂), Sodium hydroxide (NaOH), 2,4-dichlorophenol (2,4-DP), 4-aminoantipyrine (4-AP), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), peroxidase from horseradish (HRP), Hydrogen peroxide solution (H₂O₂), N-methyl-2-pyrrolidone (NMP), phenol, catechol, hydroquinone, 2-aminophenol, 2,6-dimethoxyphenol, 2-naphthol, 2-nitrophenol, 2,4,6-trichlorophenol, epinephrine (EP), norepinephrine (NP), dopamine, l-DOPA, sodium chloride (NaCl), potassium chloride (KCl), disodium phosphate (Na₂HPO₄), and potassium phosphate monobasic (KH₂PO₄) were purchased from Sigma-Aldrich (Rehovot, Israel). All materials were used as received. Milli-Q water was used to prepare all the buffers and solutions.

Acetonitrile, glacial acetic acid, and formic acid (all HPLC grade) were obtained from Sigma-Aldrich (Saint Louis, MO, US). Polymerically bonded ethylenediamine-N-propyl phase (PSA) was purchased from Varian (Palo Alto, CA, USA), while anhydrous magnesium sulfate, sodium chloride, anhydrous ammonium acetate, and C18 were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Aqueous solutions were prepared with ultrapure-water (Milli-Q Plus system; Millipore Corp., Billerica, MA, USA). Prochloraz and its metabolites (2,4,6-trichlorophenol (2,4,6-TCP), 1-propyl-1-[2-(2,4,6 trichlorophenoxy)ethyl]urea (BTS 44595), 3-formyl-1-propyl-1-[2-(2,4,6-trichlorophenoxy)ethyl]urea (BTS 44596)], and fludioxonil were of high purity grade (>99.0%), purchased from Sigma-Aldrich (Saint Louis, MO, USA). Individual stock solutions were prepared at 1000 mg/L in Acetonitrile and stored at −20 °C. The working solutions were prepared by carrying out appropriate dilutions of the stock solutions.

The composition of the stock synthetic wastewater prepared is shown in Table 1. The total carbon content was 1080 mg L^-1, 95% organic and 5% inorganic. It is a typical non-toxic and biodegradable wastewater that has been used as substrate to examine the effect of the presence of toxic compounds on the efficiency of wastewater treatment technologies [26 ,27 ]. Fresh stock solutions were prepared every three days. The following ratios of stock solution volume to water volume were used to achieve the experimental total carbon of 477, 124, 61, and 32 mg L^-1, respectively, for the UV/TiO₂ experiments: 110 mL/140 mL, 28 mL/222 mL, 14 mL/236 mL, and 7.5 mL/242.5. Phenol was supplied by Sigma Aldrich. 2-Chlorophenol (≥99% w/w), 2,4,6-Trichlorophenol (≥98% w/w), and 4-Nitrophenol (≥99% w/w) were purchased from Sigma Aldrich. 2,4-Dichlorophenol (≥99% w/w) was supplied by Acros Organics. Titanium (IV) dioxide (Aeroxide P25, nanopowder, 21 nm average primary particle size, ≥99.5% trace metals basis, 80/20 anatase to rutile weight ratio, specific surface area 50 m² g^-1) from Sigma-Aldrich was used as catalyst, whereas hydrogen peroxide (37.6% w/w) was purchased from Skat-Reactiv. Iron chloride anhydrous (≥97% w/w) was obtained from Fisher scientific.

PCE, TCE, cDCE, trans-dichloroethene (tDCE) vinyl chloride (VC), ethene (ETH), 2,4,6-trichlorophenol (2,4,6-TCP), 2,6-dichlorophenol (2,6-DCP), 2,4- dichlorophenol (2,4-DCP), chlorophenol (CP), 2,6-DBP, 3-bromophenol (3-BP), 2-bromophenol (2-BP), 2,4,6-triiodophenol (2,4,6-TIP), 2,6-diiodophenol (2,6-DIP), 2,4-diiodophenol (2,4-DIP), 2-iodophenol (2-IP) and 4-iodophenol (4-IP), phenol, 1,4-dibromobenzene (1,4-DBB), bromobenzene (BB), and benzene were purchased from Sigma-Aldrich. Sulfate (0.5 M) and lactate (0.5 M) stock solutions were prepared by filter sterilization (syringe filter, 0.2 µm, mdimembrane, Ambala Cantt, India). All other (in)organic chemicals were of analytical grade.