Anti sheep

An affinity-purified antibody against RabGAP1L was generated by immunization of rabbits with a purified peptide containing amino acids 1–235 of mouse RabGAP1L (see generation of anti-RabGAP1L antibody). Rabbit affinity-purified antibodies against total AnkB (C-terminal domain), β2-spectrin (spectrin repeats 4–9), GFP, sheep anti-AnkB (C-terminal domain) and goat anti-AnkG (C-terminal domain) were all generated in our laboratory (Ayalon et al., 2011 (link)). Other primary antibodies include, mouse anti-HA and chicken anti-GFP (Aves Labs, Tigard, OR), rabbit anti-Rab5 and anti-TGN38 (Cell Signaling Technology, Danvers, MA), mouse anti-LAMP1 (Developmental Studies Hybridoma Bank, University of Iowa, IA), rabbit anti-Rab11 (Invitrogen), rat anti-EEA1 and rabbit anti-golgin97 (Abcam, Cambridge, UK), mouse anti-α-tubulin and mouse anti-sheep/goat IgG (Thermo, Waltham, MA). Alexa488-anti-mouse/rat CD29 (Biolegend, San Diego, CA), Alexa633-WGA and Alexa568-tranferrin (Life Technologies, Carlsbad, CA) were used in internalization and recycling assays. Secondary antibodies used includes: Alexa Fluor 488 or 568–conjugated donkey anti–mouse, anti–rabbit, anti-chicken, anti-rat, anti-sheep or anti–goat purchased from Invitrogen.