Non silencing sirna

Manufactured by RiboBio

Non-silencing siRNA is a control RNA molecule designed to not target any known gene sequence. It is used to help determine the specificity of gene silencing effects in RNA interference (RNAi) experiments.

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Lab products found in correlation

P3000 reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Tgfbr2 sirna, by RiboBio (1 mentions)

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SiRNAs (265 citations)

2 protocols using non silencing sirna

Modulating NLRP3 Expression in Esophageal Cancer

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To knockdown NLRP3, KYSE-70 and TE13 cells were transfected with NLRP3-small interfering RNA (siRNA) and a non-silencing siRNA (Guangzhou RiboBio Co., Ltd.), as negative control. For RNA interference, 50 pmol siRNA was transiently transfected into the cells using 3.75 µl Lipofectamine^™ 3000 Reagent (Thermo Fisher Scientific, Inc.) and then cells were incubated for 48 h.
To overexpress NLRP3, KYSE-510 and EC9706 cells were transfected with the plasmid pCDNA3.1 (+)-NLRP3 and pcDNA-3.1(+) vector plasmid as negative control. Cells were then incubated for 18 h, and then transfection was conducted with Lipofectamine^™ 3000 Reagent and P3000^™ reagent (Thermo Fisher Scientific, Inc.) following the manufacturer's instructions.

Yu S., Yin J.J., Miao J.X., Li S.G., Huang C.Z., Huang N., Fan T.L., Li X.N., Wang Y.H., Han S.N, & Zhang L.R. (2020). Activation of NLRP3 inflammasome promotes the proliferation and migration of esophageal squamous cell carcinoma. Oncology Reports, 43(4), 1113-1124.

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Knockdown of Tgfbr2 in Chondrogenic ATDC5 Cells

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Tgfbr2-siRNA and non-silencing siRNA were purchased from Ribo (RiboBio, Guangzhou, China). Active siRNA against Tgfbr2 used in the in vitro studies had sequences 5′-CCUGUUGCCUGUGUGACUU-3′ (sense) and 3′-GGACAACGGACACACUGAA-5′ (antisense). Tgfbr2-siRNA transfection of siRNA was performed using lipofectamine transfection regent 2000 (Invitrogen, Carlsbad, CA), according to the manufacturer’s protocol. Control cells were mock transfected with lipofectamine transfection regent 2000 only. Chondrogenic ATDC5 cells were seeded in a 6-well plate until cells reached 50% confluence. The full culture medium was changed with serum-free and antibiotic-free medium at 20 min before transfection. The cells were incubated with transfection mixtures containing Tgfbr2-siRNA or non-silencing siRNA for 6 h, and then the mixtures were replaced with complete culture medium.

Wang P.E., Zhang L., Ying J., Jin X., Luo C., Xu S., Dong R., Xiao L., Tong P, & Jin H. (2018). Bushenhuoxue formula attenuates cartilage degeneration in an osteoarthritic mouse model through TGF-β/MMP13 signaling. Journal of Translational Medicine, 16, 72.

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