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Bend 3

Manufactured by GE Healthcare

The BEnd.3 is a laboratory equipment product from GE Healthcare. It is designed for core biological and analytical applications. The BEnd.3 provides foundational capabilities to support research and development workflows. Further details on its specific functions and intended use are not available.

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2 protocols using bend 3

1

Isolation and Culture of Primary Cells

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Primary human umbilical vein endothelial cells (HUVEC) were purchased from Lonza. Mouse brain endothelial cells (bEnd.3) were obtained from American Type Culture Collection (Manassas, VA). HUVEC and bEnd.3 cells were cultured as described earlier.46 (link) Human peripheral blood mononuclear cells (PBMCs) were isolated from blood from healthy donors by density gradient centrifugation using Ficoll Paque (GE Healthcare, Pittsburg, PA). The Institutional Review Board at the UT Health Science Center at Tyler approved the blood donation protocol, and the participants gave written informed consent.
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2

Isolation and Culture of Human and Mouse Endothelial Cells

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Primary human umbilical vein endothelial cells were purchased from Lonza. Mouse brain endothelial cells (bEnd.3) were obtained from American Type Culture Collection (Manassas, VA). Human umbilical vein endothelial cell and bEnd.3 cells were cultured as described earlier.45 (link) Human peripheral blood mononuclear cells (PBMCs) were isolated from blood from healthy donors by density gradient centrifugation using Ficoll Paque (GE Healthcare, Pittsburg, PA). The Institutional Review Board at the UT Health Science Center at Tyler approved the blood donation protocol, and the participants gave written informed consent.
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