Calcium chloride dihydrate CaCl 2 Á2H 2 O, sodium carbonate (Na 2 CO 3 , anhydrous), and fluorescein isothiocyanate isomer I (FITC) were purchased from Sigma-Aldrich (Germany); aprotinin from bovine lung: preparation ''Ingiprol'' (60% active centre) from RUE Belmedpreparaty (Belarus); insulin, zinc salt from Human Biosynthetic, IBCh RAS, Russia; and catalase from bovine liver (C-1345) from Sigma, Germany. The water used in all experiments was prepared using a Millipore Milli-Q purification system and had a resistivity higher than 18.2 MO cm.
Calcium chloride dihydrate cacl2 2h2o
Manufactured by Merck Group
Sourced in Germany, Japan, United States
Calcium chloride dihydrate (CaCl2·2H2O) is a chemical compound that consists of calcium, chloride, and water. It is a crystalline solid that is used as a laboratory reagent and in various industrial applications. The core function of calcium chloride dihydrate is to provide a source of calcium ions and chloride ions for various chemical reactions and processes.
Automatically generated - may contain errors
Lab products found in correlation
Milli q purification system, by Merck Group (3 mentions)
Fluorescein isothiocyanate isomer 1 fitc, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Catalase from bovine liver, by Merck Group (1 mentions)
Ethanol for spectroscopy, by Merck Group (1 mentions)
Fuming hydrochloric acid, by Merck Group (1 mentions)
0.2 μm titan3 sterile filters, by Thermo Fisher Scientific (1 mentions)
Dextromethorphan hydrobromide, by Merck Group (1 mentions)
Chloroquine diphosphate, by Merck Group (1 mentions)
Acetic acid ch3cooh, by Thermo Fisher Scientific (1 mentions)
Sodium carbonate na2co3, by Merck Group (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
Propidium iodide, by Dojindo Laboratories (1 mentions)
1 3 diphenylisobenzofuran dpbf, by Merck Group (1 mentions)
Calcein am cam, by Dojindo Laboratories (1 mentions)
Cell counting kit 8 cck 8, by MedChemExpress (1 mentions)
Singlet oxygen sensor green (sosg), by Thermo Fisher Scientific (1 mentions)
Ammonium bicarbonate nh4hco3, by Merck Group (1 mentions)
Nuclear dye 4 6 diamidino 2 phenylindole dapi, by Beyotime (1 mentions)
10 protocols using calcium chloride dihydrate cacl2 2h2o
1
Preparation and Characterization of Fluorescent Insulin
2
Enzymatic Synthesis of Calcium Carbonate
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Calcium chloride dihydrate CaCl 2 Á2H 2 O (Sigma-Aldrich, Japan); sodium carbonate Na 2 CO 3 anhydrous (Sigma-Aldrich, Germany); catalase from bovine liver -3809 units per mg solid (Sigma, Germany (C-1345)); 0.05 M glycine buffer (pH 9.0), 0.05 M phosphate buffer (pH 7.0), ethylenediamine-tetraacetic acid (EDTA) and hydrogen peroxide were purchased from Sigma, USA; water used in all experiments was prepared via a Millipore Milli-Q purification system and had a resistivity higher than 18.2 MO cm.
3
Thermo-responsive Microgel Preparation
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The materials used include calcium chloride
dihydrate (CaCl2·2H2O; Sigma-Aldrich, Japan),
sodium carbonate (Na2CO3, anhydrous; Sigma-Aldrich,
Germany), phosphate buffered saline (PBS buffer, tablets, 0.01 M;
Sigma, USA), fluorescein isothiocyanate-DEX [mol wt (MW): 10, 70,
and 500 kDa; Sigma, USA], rhodamine 6G (Rho6G) chloride (Sigma, England),
ethanol for spectroscopy (99.9%; Merck, Germany), Fluoresbrite calibration
grade 6.0 μm microspheres (Polysciences Inc., UK), and hydrochloric
acid fuming (37%, 0.05 M in all experiments; Merck, Germany). The
water used in all experiments was prepared via a Millipore Milli-Q
purification system and had a resistivity higher than 18.2 MΩ-cm.
Poly(N-isopropylacrylamide)-4-acryloylbenzophenone
(pNIPAM-ABP) was kindly provided by Dr. Leonid Ionov, Germany. Briefly,
pNIPAM-ABP was synthesized from 4-benzophenone acrylate (0.28 g, 1.12
mmol) and NIPAM (6 g, 51.57 mmol) dissolved in 30 mL of ethanol. The
mixture was purged with nitrogen for 30 min. The polymerization was
carried out at 70 °C under a nitrogen atmosphere with mechanical
stirring overnight. The cooled mixture was poured into 750 mL of diethyl
ether, and the precipitate was filtered and dried in vacuum at 40 °C.
dihydrate (CaCl2·2H2O; Sigma-Aldrich, Japan),
sodium carbonate (Na2CO3, anhydrous; Sigma-Aldrich,
Germany), phosphate buffered saline (PBS buffer, tablets, 0.01 M;
Sigma, USA), fluorescein isothiocyanate-DEX [mol wt (MW): 10, 70,
and 500 kDa; Sigma, USA], rhodamine 6G (Rho6G) chloride (Sigma, England),
ethanol for spectroscopy (99.9%; Merck, Germany), Fluoresbrite calibration
grade 6.0 μm microspheres (Polysciences Inc., UK), and hydrochloric
acid fuming (37%, 0.05 M in all experiments; Merck, Germany). The
water used in all experiments was prepared via a Millipore Milli-Q
purification system and had a resistivity higher than 18.2 MΩ-cm.
Poly(N-isopropylacrylamide)-4-acryloylbenzophenone
(pNIPAM-ABP) was kindly provided by Dr. Leonid Ionov, Germany. Briefly,
pNIPAM-ABP was synthesized from 4-benzophenone acrylate (0.28 g, 1.12
mmol) and NIPAM (6 g, 51.57 mmol) dissolved in 30 mL of ethanol. The
mixture was purged with nitrogen for 30 min. The polymerization was
carried out at 70 °C under a nitrogen atmosphere with mechanical
stirring overnight. The cooled mixture was poured into 750 mL of diethyl
ether, and the precipitate was filtered and dried in vacuum at 40 °C.
4
Purification and Characterization of Extracellular Vesicles
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Sodium dithionite (Na2S2O4), glutaraldehyde (C5H8O2) (70 wt%), sodium cyanoborohydride (NaCNBH3), sodium lactate (NaC3H5O3), N‐acetyl‐L‐cysteine (NALC, C5H9NO3S), and calcium chloride dihydrate (CaCl2·2H2O) were purchased from Sigma Aldrich. Sodium chloride (NaCl), potassium chloride (KCl), sodium phosphate monobasic (NaH2PO4), sodium phosphate dibasic (Na2HPO4), sodium hydroxide (NaOH), and 0.2 μm Titan3 sterile filters were purchased from Fisher Scientific. Hollow fiber tangential flow filtration (TFF) modules N02‐P500‐05‐N (polysulfone [PS], 500 kDa pore size), N02‐S20U‐05‐N (polyethersulfone [PES], 0.2 µM pore size), and N02‐E65U‐07‐N (PES, 0.65 µM pore size) were purchased from Repligen. The Liqui‐CelTM EXF Series G420 Membrane Contactor was purchased from 3M. A minicentrifuge (50‐090‐100, with a working speed of 6000 rpm and maximum speed of 6600 rpm) was obtained from Fisher Scientific. Expired human leukoreduced, packed RBC units were acquired from Transfusion Services at the Wexner Medical Center, Canadian Blood Services, and Zen‐Bio Inc.
5
Preparation and Characterization of Ionic Solutions
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Sodium Chloride (NaCl, CAS: 7647-15-5), potassium chloride (KCl, CAS: 7447-40-7) and sodium acetate (CH3COONa, CAS: 127-09-3) were obtained from Caledon Laboratories (Ontario, Canada) while the calcium chloride dihydrate (CaCl2.2H2O, CAS: 10035-04-8) was from Sigma (Steinheim, Germany) and the acetic acid (CH3COOH, CAS:64-19-7) was purchased from Fischer Scientific, USA. Distilled water was used for dissolving the reagents. Dextromethorphan hydrobromide (CAS: 6700-34-1) and chloroquine diphosphate (CAS: 50-63-5) were obtained from Sigma- Aldrich Co. (Missouri, USA) whereas the dextromethorphan soft gel capsules (Robitussin®) were a product of Pfizer (Ontario, Canada) from the lot: DP5155 (Expiration Date: 02/2023).
6
Mineralization of PCL Scaffolds
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The procedure for the formation of CaCO3 on the PCL scaffolds was similar to that described elsewhere.7 (link) Briefly, CaCO3 particles were crystallised on the surface of polymer fibres from a mixture of 1 M-CaCl2-saturated and 1 M-Na2CO3-saturated solutions. The CaCl2 and Na2CO3 solutions were prepared using calcium chloride dihydrate (CaCl2·2H2O; Sigma-Aldrich Chemie, Germany) and sodium carbonate (Na2CO3; Sigma-Aldrich Chemie, Germany), respectively. First, the PCL scaffold samples (0.5 × 1 cm2) were treated with 1 mL of CaCl2 solution in an ultrasonic bath until complete wetting was achieved. Complete wetting of scaffolds that were pretreated with the O2 plasma was achieved immediately, whereas that of the other scaffolds was achieved in about 10 min. Subsequently, 1 mL of the Na2CO3 solution was added to the system upon ultrasonication, after which the system was subjected to ultrasound for 30 s. Finally, the samples immersed in the solution were kept out for 60 s to complete the crystallisation process and washed twice with deionised (DI) water. The mineralisation of the PCL scaffolds was performed twice and completed with drying of the samples at 40–45 °C for 30–40 min.
7
Alginate Hydrogel Formation Protocol
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Sodium alginate and calcium chloride dihydrate (CaCl2·2H2O) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Three mL disposable syringes were purchased from Becton Dickinson (Franklin Lakes, NJ, USA). Some 17 gauge and 22 gauge blunt-tip needles were purchased from Hamilton Company (Reno, NV, USA). Multiple gauged premium Kanthal wires were purchased from Amazon or Walmart.
8
Lipid-based Photosensitizer Delivery
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Ethanol (anhydrous, >99.7) and chloroform were obtained from Chongqing Chuandong Chemical Co. Ltd. (Chongqing, China). Calcium chloride dihydrate (CaCl2·2H2O), ammonium bicarbonate (NH4HCO3) and 1.3-diphenylisobenzofuran (DPBF) were purchased from Sigma Aldrich (MO, USA). HMME was purchased from Shanghai D&B Biotechnology Co., Ltd. (Shanghai, China). Nuclear dye 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from Beyotime Biotechnology (Shanghai, China). Cell Counting Kit-8 (CCK-8) was bought from MedChemExpress (New Jersey, USA). Calcein-AM (CAM) and propidium iodide (PI) were obtained from Dojindo (Japan). Proliferating cell nuclear antigen (PCNA) antibody was obtained from Servicebio Technology Co., Ltd (Wuhan, China). Singlet oxygen sensor green (SOSG) was purchased from Thermo Fisher Scientific (California, USA). 1.2-dioleoyl-sn-glycerol-3-phosphate (sodium salt) (DOPA) was purchased from Avanti Polar Lipids, Inc. (Alabaster, Alabama, USA).1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000 (DSPE-PEG2000), 1.2-dipalmitoyl-sn-glycero-3-phospha-tidylcholine (DPPC) and cholesterol were synthesized by Xi’an Ruixi Biological Technology Co., Ltd (Xi’an, China).
9
Synthesis of Inorganic Nanoparticles
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Ethylenediaminetetraacetic acid disodium salt dehydrate was purchased from AppliChem (Darmstadt, Germany). Tetrachloroauric (III) acid was purchased from ROTH (Karlsruhe, Germany). Potassium carbonate and rhodamine B were purchased from Fluka (Buchs, Germany). Calcium chloride dihydrate CaCl2·2H2O, sodium carbonate Na2CO3 anhydrous, ascorbic acid, dimethylaminopyridine (DMAP), and bovine serum albumin (Fraction V) were purchased from Sigma (Hamburg, Germany). Other reagents were from Sigma and were at least of analytical grade. Sterile deionized ultrafiltred water was used from a Millipore purification system (Darmstadt, Germany).
10
LDH Synthesis and Fluoride Evaluation
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Calcium chloride dihydrate (CaCl2•2H2O; Sigma-Aldrich, UK), magnesium chloride (MgCl2; Sigma-Aldrich, UK) and aluminium chloride (AlCl3; Fluka Analytical) reagents, with a >99% purity, were used to produce LDH (see below). Fluoride absorption and release (over six cycles) was also compared from two commercial resin composites (Tetric and Tetric EvoCeram from Ivoclar Vivadent, Lichtenstein). These commercial products were selected as they closely matched the experimental composite matrix (see below). However, both commercial products additionally contained fillers (81 wt%). The full composition can be observed in the supplementary material).
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