C fos sirna

Manufactured by Santa Cruz Biotechnology

C-Fos siRNA is a synthetic, double-stranded RNA molecule designed to target and suppress the expression of the c-Fos gene. The c-Fos gene is a proto-oncogene that plays a role in cellular processes such as proliferation, differentiation, and transformation.

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C-Fos (129 citations) SiRNAs (95 citations) C-siRNA (7 citations)

3 protocols using c fos sirna

Silencing of c-Jun and c-Fos in Cell Line

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When the cells reached 60% confluence, scramble siRNA, c-Jun siRNA and c-Fos siRNA obtained from Santa Cruz Biotechnology, Inc. were transfected into the cell line, respectively, using Lipofectamine RNA iMAX transfection reagent (Invitrogen). After 72 h of transfection, proteins were harvested for subsequent tests.

Hao Y., Zhu L., Yan L., Liu J., Liu D., Gao N., Tan M., Gao S, & Lin B. (2017). c-Fos mediates α1, 2-fucosyltransferase 1 and Lewis y expression in response to TGF-β1 in ovarian cancer. Oncology Reports, 38(6), 3355-3366.

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Molecular Signaling Pathways in Neurodegeneration

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Antibodies against the following proteins were used in this study: Akt, phospho-Akt (Ser473), mTOR, phosphor mTOR, p70 S6 kinase, phospho p70 S6 kinase, STAT3, phospho- STAT3 (Tyr705), and LC3II from Cell Signaling Technology, c-fos, ERK, phospho ERK and β-amyloid (immunohistochemistry) from Santa Cruz Biotechnology, Insulin receptor (Y1158) from Abcam Cambridge, β-amyloid (Immunoblot analysis) from BioLegend, Donkey anti-Mouse IgG from Thermo Fisher Scientific, β-actin from EnoGene, and horseradish peroxidase-conjugated secondary antibodies from Enzo Life Sciences. β-amyloid (human, 1–42) was purchased from Invitrogen. LY294002 and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma. PD98059 was purchased from Calbiochem. Recombinant human LIF was purchased from Peprotech. c-fos siRNA was purchased from Santa Cruz Biotechnology.

Lee H.J., Lee J.O., Lee Y.W., Kim S.A., Seo I.H., Han J.A., Kang M.J., Kim S.J., Cho Y.H., Park J.J., Choi J.I., Park S.H, & Kim H.S. (2019). LIF, a Novel Myokine, Protects Against Amyloid-Beta-Induced Neurotoxicity via Akt-Mediated Autophagy Signaling in Hippocampal Cells. International Journal of Neuropsychopharmacology, 22(6), 402-414.

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Silencing c-Fos Expression in Cells

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c-Fos siRNA (catalog number sc-29221) and negative control siRNA (catalog number sc-37007) were purchased from Santa Cruz Biotechnology, Inc. For the transfection procedure, cells were grown to 70%–80% confluence, and c-Fos siRNA and control siRNA were transfected using LipofetamineTM2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. At 24 h post-transfection, the cells received different treatments. Then, cells were harvested for Western blot analyses and gelatin zymography.

Yin X., Zhou L., Han F., Han J., Zhang Y., Sun Z., Zhao W., Wang Z, & Zheng L. (2017). Beta-adrenoceptor Activation by Norepinephrine Enhances Lipopolysaccharide-induced Matrix Metalloproteinase-9 Expression Through the ERK/JNK-c-Fos Pathway in Human THP-1 Cells. Journal of Atherosclerosis and Thrombosis, 24(1), 55-67.

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