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Rat mouse pinp eia kit

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The Rat/Mouse PINP EIA kit is a laboratory assay used to measure the levels of procollagen type I N-terminal propeptide (PINP) in rat and mouse samples. PINP is a biomarker that reflects bone formation activity.

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Lab products found in correlation

μct 35, by Scanco (3 mentions) Ratlaps eia kit, by Immunodiagnostic Systems (3 mentions) Ratlaps elisa, by Immunodiagnostic Systems (2 mentions) Mousetrap tracp 5b elisa kit, by Immunodiagnostic Systems (1 mentions) Minicollect tube, by Greiner (1 mentions) Ratlaps ctx 1 eia, by Immunodiagnostic Systems (1 mentions) Ratlaps ctx 1 eia kit, by Immunodiagnostic Systems (1 mentions) Ratlaps, by Immunodiagnostic Systems (1 mentions) Eia kit, by Immunodiagnostic Systems (1 mentions) Ratlaps ctx 1 elisa kit, by Immunodiagnostic Systems (1 mentions) Osteocalcin enzyme immunoassay kit, by Thermo Fisher Scientific (1 mentions) Human lambda elisa kit, by Fortis Life Sciences (1 mentions) Sky microplate spectrophotometer, by MultiSciences Biotech (1 mentions)

12 protocols using rat mouse pinp eia kit

1

Fasting Serum Collection for Bone Markers

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Serum was collected from mice after 6 hours of fasting for CTX-I and PINP assays. The assays were performed with the RatLaps ELISA or Rat/Mouse PINP EIA Kit (both from Immunodiagnostic Systems, Ltd.).
Shi Y., Liao X., Long J.Y., Yao L., Chen J., Yin B., Lou F., He G., Ye L., Qin L, & Long F. (2021). Gli1+ progenitors mediate bone anabolic function of teriparatide via Hh and Igf signaling. Cell reports, 36(7), 109542.
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2

Serum Biomarkers in Aged Mice

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Blood was collected from 8 week old male mice by cardiac puncture. Serum was separated by centrifugation at 1500 x g for 15 minutes. Serum levels of collagen N-propeptide (PINP) and tartrate resistant acid phosphatase (TRAcP) were measured by Rat/Mouse PINP EIA kit or MouseTRAP™ (TRAcP 5b) ELISA kit (Immunodiagnostic Systems, Tyne & Wear, UK), respectively, using the manufacturer’s protocol.
Cabral W.A., Fratzl-Zelman N., Weis M., Perosky J.E., Alimasa A., Harris R., Kang H., Makareeva E., Barnes A.M., Roschger P., Leikin S., Klaushofer K., Forlino A., Backlund P.S., Eyre D.R., Kozloff K.M, & Marini J.C. (2020). Substitution of murine type I collagen A1 3-hydroxylation site alters matrix structure but does not recapitulate osteogenesis imperfecta bone dysplasia. Matrix biology : journal of the International Society for Matrix Biology, 90, 20-39.
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3

Serum Biomarker Measurement Protocol

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Blood was collected retro-orbitally under inhalation of a 2% isoflurane/oxygen mix anesthesia immediately prior to sacrifice. Serum was obtained by centrifugation of blood in a MiniCollect tube (catalog no. 450472, Greiner Bio-one GmbH, Austria). The serum levels of TRAcP-5b, CTx-I, and PINP were measured by a mouse TRAP (TRAcP 5b) kit (SB-TR103), RatLaps (CTx-I) EIA (AC-06F1), and rat/mouse PINP EIA kit (AC-33F1) from Immunodiagnostic Systems following their instructions.
Das B.K., Wang L., Fujiwara T., Zhou J., Aykin-Burns N., Krager K.J., Lan R., Mackintosh S.G., Edmondson R., Jennings M.L., Wang X., Feng J.Q., Barrientos T., Gogoi J., Kannan A., Gao L., Xing W., Mohan S, & Zhao H. (2022). Transferrin receptor 1-mediated iron uptake regulates bone mass in mice via osteoclast mitochondria and cytoskeleton. eLife, 11, e73539.
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4

Quantitative Determination of Bone Markers

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Quantitative determination of PINP and CTX-I was performed using a Rat/Mouse PINP EIA Kit (AC-33F, Immunodiagnostic systems, Tyne and Wear, UK) and a RatLaps CTX-I EIA Kit (AC-06F1, Immunodiagnostic systems), respectively, according to the manufacturer’s instructions.
Yahiro Y., Maeda S., Morikawa M., Koinuma D., Jokoji G., Ijuin T., Komiya S., Kageyama R., Miyazono K, & Taniguchi N. (2020). BMP-induced Atoh8 attenuates osteoclastogenesis by suppressing Runx2 transcriptional activity and reducing the Rankl/Opg expression ratio in osteoblasts. Bone Research, 8, 32.
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5

Fasting Serum Collection for Bone Markers

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Serum was collected from mice after 6 hours of fasting for CTX-I and PINP assays. The assays were performed with the RatLaps ELISA or Rat/Mouse PINP EIA Kit (both from Immunodiagnostic Systems, Ltd.).
Shi Y., Liao X., Long J.Y., Yao L., Chen J., Yin B., Lou F., He G., Ye L., Qin L, & Long F. (2021). Gli1+ progenitors mediate bone anabolic function of teriparatide via Hh and Igf signaling. Cell reports, 36(7), 109542.
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6

Bone Turnover Markers Measurement

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Serum P1NP concentration was measured with a Rat/Mouse PINP EIA kit (Immunodiagnostic Systems, Boldon, UK). Urine DPD concentration was measured with an Osteolinks DPD kit (Quidel, San Diego, CA, USA). Urine creatinine (Cr) concentration was measured with a 7180 Autoanalyzer (Hitachi High-Technologies Corporation, Tokyo, Japan).
Makino A., Takagi H., Takahashi Y., Hase N., Sugiyama H., Yamana K, & Kobayashi T. (2018). Abaloparatide Exerts Bone Anabolic Effects with Less Stimulation of Bone Resorption-Related Factors: A Comparison with Teriparatide. Calcified Tissue International, 103(3), 289-297.
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7

Bone Biomarker Measurement Protocol

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The following kits were used, following manufacturer’s instructions: CrossLaps (CTX-I) ELISA kit, RatLaps (CTX-I) EIA kit, Rat/Mouse PINP EIA kit (Immunodiagnostic Systems, Tyne and Wear, UK).
Puissant E., Gilis F., Tevel V., Vandeweerd J.M., Flamion B., Jadot M, & Boonen M. (2022). Hyaluronidase 1 deficiency decreases bone mineral density in mice. Scientific Reports, 12, 10142.
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8

μCT-based Bone Histomorphometry and Serum Markers

Cited 1 time
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μCT was performed using a Scanco μCT-35 instrument (SCANCO Medical) as described (Wei et al., 2010 (link)). Histomorphometry were performed as described (Wan et al., 2007a (link); Wei et al., 2011 (link)). Serum CTX-1 and P1NP were measured with RatLaps™ EIA kit and Rat/Mouse PINP EIA kit (Immunodiagnostic Systems). For LPS-induced bone loss, we i.p. injected mice at 6-8 weeks of age with PBS or LPS (5mg/kg) at day 0 and day 4, and sacrificed them at day 7.
Jin Z., Wei W., Yang M., Du Y, & Wan Y. (2014). Mitochondrial Complex I Activity Suppresses Inflammation and Enhances Bone Resorption by Tipping the Balance of Macrophage-Osteoclast Polarization. Cell metabolism, 20(3), 483-498.
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9

Bone Volume Evaluation via μCT and Histomorphometry

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μCT was performed to evaluate bone volume and architecture using a Scanco μCT-35 instrument (SCANCO Medical) as described (Wei et al., 2012 (link); Wei et al., 2010 (link)). Bone histomorphometry was performed as described (Wei et al., 2012 (link)). As a bone resorption marker, serum CTX-1 was measured with the RatLaps™ EIA kit (Immunodiagnostic Systems) (Wei et al., 2012 (link)). As a bone formation marker, serum PINP was measured with the Rat/Mouse PINP EIA kit (Immunodiagnostic Systems) (Wei et al., 2012 (link)).
Wang X., Wei W., Krzeszinski J.Y., Wang Y, & Wan Y. (2015). A Liver-Bone Endocrine Relay by IGFBP1 Promotes Osteoclastogenesis and Mediates FGF21-Induced Bone Resorption. Cell metabolism, 22(5), 811-824.
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10

Comprehensive Bone Analysis Protocol

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μCT was performed using a Scanco μCT-35 instrument (Scanco Medical, Brüttisellen, Switzerland) as described (Wei et al., 2010 (link)). Histomorphometry were performed as described (Wan et al., 2007 (link); Wei et al., 2011 (link)). Serum CTX-1 bone resorption marker and P1NP bone formation marker were measured with RatLaps EIA kit and Rat/Mouse PINP EIA kit (Immunodiagnostic Systems, Tyne & Wear, United Kingdom), respectively. To analyze osteocyte gene expression, mouse femur was cut off at both ends to allow marrow cells to be flushed out with media. It was then soaked in PBS and spun down to remove residual marrow cells, and snap frozen in liquid nitrogen, stored at −80°C until RNA extraction.
Li X., Wei W., Huynh H., Zuo H., Wang X, & Wan Y. (2015). Nur77 prevents excessive osteoclastogenesis by inducing ubiquitin ligase Cbl-b to mediate NFATc1 self-limitation. eLife, 4, e07217.
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