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2 protocols using dichloromethane dcm

1

Comprehensive PCB Congener Quantification

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A standard PCB mixture containing 14 PCB congeners (PCB-18, PCB-28, PCB-29, PCB-43, PCB-52, PCB-101, PCB-118, PCB-138, PCB-142, PCB-153, PCB-170, PCB-180, PCB-194 and PCB-209) was obtained from Supelco Inc. (Bellefonte, PA, USA), while a mixed standard containing six isotopically labelled 13C12PCBs (13C12PCB-28, 52, 118, 153, 180 and 209) was obtained from Cambridge Isotope Laboratories, Inc. (USA). The PCB congener choice covered the seven indicator PCBs. n-Hexane, dichloromethane (DCM) and acetone (pesticide grade) were obtained from BDH, Poole, UK, while anhydrous sodium sulfate, copper powder, Florisil and silica gel were purchased from Merck (Darmstadt, Germany).
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2

Extraction and Analysis of Ipomoea aquatica

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Methanol (MeOH) (HPLC grade), acetonitrile (ACN) (HPLC grade), glacial acetic acid (AA) (100 %), dimethyl sulfoxide (DMSO), and anisaldehyde were purchased from Merck (Darmstadt, Germany); trifluoroacetic acid (TFA) (≥98%), diethylamine (DEA) (≥99.5%) and methanol-d4 (99.8% D) from Sigma-Aldrich (St. Louis, MO, USA); MeOH AnalaR NORMAPUR ® (analytical reagent) from VWR (Fontenay-sous-Bois, France), n-hexane from SDFCL (Maharashtra, India), dichloromethane (DCM) (99.5%) from BDH (Leicestershire, UK), and ethyl acetate (EA) (99.9%) from ROMIL (Cambridge, UK); sulfuric acid (H2SO4) (96% solution in water) was obtained from Acros Organics (New Jersey, USA) and ultrapure water was produced by an Arium pro ultrapure water system (Sartorius, Gottingen, Germany). The fresh plants of Ipomoea aquatica (aerial parts) were collected in Darfur, West Sudan in March 2010. A voucher specimen was deposited in the Herbarium of the Botany Department, Faculty of Science, University of Khartoum, Sudan.
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