All the reagents were used without further purification unless otherwise specified. Solvents were dried and redistilled prior to use in the usual manner. Analytical TLC was performed using silica gel HF254. Preparative column chromatography was performed with silica gel H. 1H and 13C NMR spectra were recorded on a Bruker Advance III 600 MHz spectrometer. HRMS were obtained on a Thermofisher LTQ-Obitrap XL.
Advance 3 600 mhz
Manufactured by Bruker
Sourced in United States
The Advance III 600 MHz is a nuclear magnetic resonance (NMR) spectrometer from Bruker. It operates at a frequency of 600 MHz and is designed for high-resolution NMR analysis.
Automatically generated - may contain errors
Lab products found in correlation
Ltq obitrap xl, by Thermo Fisher Scientific (1 mentions)
Rotavapor r 3000r, by Büchi (1 mentions)
Rp 8 f254, by Merck Group (1 mentions)
Ltq orbitrap xl hybrid mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Drx 400, by Bruker (1 mentions)
Zetasizer nano zs instrument, by Malvern Panalytical (1 mentions)
Nicolet is10, by Thermo Fisher Scientific (1 mentions)
Smartprobe, by Bruker (1 mentions)
Spectrum two, by PerkinElmer (1 mentions)
8 protocols using advance 3 600 mhz
1
Purification and Characterization of Organic Compounds
2
Isolation and Characterization of Natural Compounds
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Chemicals and reagents were from Merck (Darmstadt, Germany). Evaporation of solvents was performed on a vacuum rotary evaporator (Rotavapor R-3000r, Buchi, Switzerland). FCPC was carried out on a Kromaton instrument equipped with a 1000-ml column, adjustable rotation of 200–2000 rpm and a preparative Laboratory Alliance pump with a pressure safety limit of 50 bar. NMR spectra in MeOD were recorded at 400 and 600 MHz (Bruker Advance III 600 MHz and DRX 400). 2D NMR experiments, including correlation spectroscopy (COSY), heteronuclear single-quantum correlation (HSQC) and heteronuclear multiple-bond correlation (HMBC) were performed using standard Bruker microprograms. Electrospray ionisation mass spectrometry (ESI-MS) experiments were performed on a LTQ-Orbitrap XL hybrid mass spectrometer (Thermo-Scientific, Bremen, Germany). Analytical TLC was performed on Merck Kieselgel 60 F254 or RP-8 F254 plates. Spots were visualized by UV light (254 and 365 nm) or by spraying with sulfuric vanillin. The plates were then heated for 2 min at 110°C. Preparative TLC was conducted on PLC Silica gel 60 F254 plates (1 mm). The selected zones were scraped and extracted with ethyl acetate to separate the corresponding compounds. Column chromatography was performed on silica gel 70–230 mesh (63–200 μm). Size exclusion chromatography was performed on Sephadex LH-20.
3
High-resolution NMR Spectroscopy of Biomolecules
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High-resolution NMR spectroscopy was performed using a Bruker Advance III 600 MHz spectrometer equipped with a TCI-H/C/N triple resonance cryoprobe. All experiments were performed at 25°C. Water suppression was achieved by the Watergate sequence. Spectra were processed using TopSpin 2.1 software. One-dimensional proton (1D 1H) NMR spectra were recorded both in 50 mM sodium phosphate, 50 mM NaCl (pH 8.0) and in 50 mM Tris-HCl, 50 mM NaCl (pH 8.0). The samples were prepared in a final volume of 500 μL containing 5% (v/v) D2O as field lock.
4
hGH-IBs Refolding and Purification
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hGH-IBs was expressed in E. coli cultivated in M9 medium with 15N ammonium chloride. The washed IBs were solubilized at a concentration of 2 mg hGH/mL at 2.4 kbar and pH 10.0 in the presence of 0.25 M Arg and 10 mM DTT. The protein was then diluted tenfold in Tris HCl 50 mM containing 2 mM GSSG and incubated for 48 h at 4 °C for refolding. The protein was then dialyzed against Tris HCl 25 mM pH 7.4 and concentrated. The monomers were separated from the higher molecular weight isoforms by HPSEC and concentrated again to a concentration of 109 µM. NMR spectra were recorded at 298.15 K on a Bruker Advance III 600 MHz spectrometer operating at a magnetic field of 14.09 T and utilizing an inverse TBI probe with 5 mm.
5
Proton NMR Characterization of Polymer Scaffolds
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Proton nuclear magnetic resonance (1H-NMR) was applied to confirm the complete removal of solvent (chloroform) from the cast films and printed scaffolds. P30 leached, P100 leached and NP scaffolds (30 mg) were dissolved in CD2Cl2 and 1H NMR spectra were acquired at 298 K on a Bruker Advance III 600 MHz spectrometer equipped with a 5 mm Broadband Observer probe. 1H-NMR chemical shifts are reported in parts per million (ppm) relative to the residual protonated solvent peak.
6
Polymer Characterization by NMR, FTIR, and DLS
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1H NMR spectra
were recorded on a Bruker Advance III 600 MHz spectrometer in deuterated
solvents. FTIR spectra were recorded using a ThermoScientific Nicolet
iS10 spectrometer. Gel permeation chromatography (GPC) analysis was
performed using a Malvern OMNISEC CHR7100 chromatograph at room temperature
with a right angle light scattering (RALS) detector and a flow rate
of 0.8 mL/min. A 0.1 M NaNO3 aqueous solution containing
20% v/v acetonitrile was used as an eluent. The molecular weights
of the polymers were calculated based on poly(4-styrenesulfonate)
standard calibration. A Malvern Zetasizer Nano ZS instrument working
at 173° detection angle was used in dynamic light scattering
(DLS) measurements that were performed at 25 °C. General purpose
mode was used as a size distribution analysis algorithm, and the reported
data represent the averages from three series of measurements (10–100
runs each) and their standard deviations (mean ± SD, n = 3).
were recorded on a Bruker Advance III 600 MHz spectrometer in deuterated
solvents. FTIR spectra were recorded using a ThermoScientific Nicolet
iS10 spectrometer. Gel permeation chromatography (GPC) analysis was
performed using a Malvern OMNISEC CHR7100 chromatograph at room temperature
with a right angle light scattering (RALS) detector and a flow rate
of 0.8 mL/min. A 0.1 M NaNO3 aqueous solution containing
20% v/v acetonitrile was used as an eluent. The molecular weights
of the polymers were calculated based on poly(4-styrenesulfonate)
standard calibration. A Malvern Zetasizer Nano ZS instrument working
at 173° detection angle was used in dynamic light scattering
(DLS) measurements that were performed at 25 °C. General purpose
mode was used as a size distribution analysis algorithm, and the reported
data represent the averages from three series of measurements (10–100
runs each) and their standard deviations (mean ± SD, n = 3).
7
NMR Spectroscopy of Spider Silk Extracts
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NMR spectra were acquired
on Bruker Advance III 600 MHz spectrometers with a 5 mm inverse detection
cryoprobe or a 5 mm broadband observe detection SmartProbe, both equipped
with a z gradient. Extracts for NMR were prepared
by treating spider silk (50 mg) with deuterium oxide (D2O), ethanol-d6, or DMSO-d6 in glass vials overnight. Spectra from the D2O extract were acquired with a 1D NOESY experiment with presaturation
(Bruker pulse sequence noesygppr1d) to suppress the
residual water signal. Spectra were recorded at 25 °C and were
processed with TopSpin 3.6.1. 1H chemical shifts were referenced
to solvent signals (HDO 4.77 ppm; ethanol-d6 1.11 and 3.56 ppm; and DMSO-d6 2.50
ppm). Signals were assigned with the help of TOCSY and HSQC experiments.
on Bruker Advance III 600 MHz spectrometers with a 5 mm inverse detection
cryoprobe or a 5 mm broadband observe detection SmartProbe, both equipped
with a z gradient. Extracts for NMR were prepared
by treating spider silk (50 mg) with deuterium oxide (D2O), ethanol-d6, or DMSO-d6 in glass vials overnight. Spectra from the D2O extract were acquired with a 1D NOESY experiment with presaturation
(Bruker pulse sequence noesygppr1d) to suppress the
residual water signal. Spectra were recorded at 25 °C and were
processed with TopSpin 3.6.1. 1H chemical shifts were referenced
to solvent signals (HDO 4.77 ppm; ethanol-d6 1.11 and 3.56 ppm; and DMSO-d6 2.50
ppm). Signals were assigned with the help of TOCSY and HSQC experiments.
8
Spectroscopic Analysis of Methyl Red
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The Perkin Elmer Spectrum Two instrument (103385; Waltham, MA, USA) was used to conduct the FTIR analysis of the methyl red before and after bacterial treatment while the purified fraction obtained through silica gel column after TLC profiling was analyzed by Proton (1H) and Carbon 13 (13C) NMR (Advance III 600 MHZ, Cryoprobe, Triple Resonance Channel; Bruker, Billerica, MA, USA) for confirmation and structure elucidation.
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