The abdominal aortae of the 23 surviving rats in each group from the pretreatment protocol were harvested and cut using a cryostat (CM1950; Leica) into 8-μm-thick sections. The sections were fixed with 4% paraformaldehyde in phosphate-buffered saline (; pH 7.4) for 10 minutes at room temperature. After rinsing with phosphate-buffered saline, the sections were preincubated with 10% normal goat serum (Nichirei Biosciences, Tokyo, Japan) and incubated overnight at 4°C with mouse anti-CD 68 (Abcam, Cambridge, UK), rabbit anti-MMPs (Abnova, Taipei, Taiwan), rabbit anti-hypoxia-inducible factor (HIF)-1α (Novus Biologicals, Littleton, Colo), and antipimonidazole mouse IgG1 monoclonal antibody (Hypoxyprobe, Inc, Burlington, Mass). Immunoreactivity was visualized using Alexa Fluor 594-conjugated anti-mouse immunoglobulin G and Alexa Fluor 488-conjugated anti-rabbit immunoglobulin G (Molecular Probes, Invitrogen, Carlsbad, Calif). All Alexa-fluoroconjugated secondary antibodies were diluted 200-fold. The slides were mounted in a glycerol-based Vectashield medium (Vector Laboratories, Burlingame, Calif).
Alexa fluor 488 conjugated anti rabbit immunoglobulin g
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexa Fluor 488-conjugated anti-rabbit immunoglobulin G is a fluorescently labeled secondary antibody used for detection in immunoassays. It binds to rabbit primary antibodies and emits green fluorescence when excited.
Automatically generated - may contain errors
Lab products found in correlation
Normal goat serum (ngs), by Nichirei Biosciences (2 mentions)
Alexa fluor 594 conjugated anti mouse immunoglobulin g, by Thermo Fisher Scientific (2 mentions)
Vectashield medium, by Vector Laboratories (2 mentions)
Cm1950, by Leica (1 mentions)
Mouse anti cd68, by Abcam (1 mentions)
Rabbit anti hypoxia inducible factor 1α, by Novus Biologicals (1 mentions)
2 protocols using alexa fluor 488 conjugated anti rabbit immunoglobulin g
1
Aortic Tissue Immunohistochemistry Protocol
2
Histological Analysis of Elastin and Collagen
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Tissue sections (8 μm) were fixed with 4% paraformaldehyde in PBS (pH 7.4) for 10 min at room temperature and stained routinely with Elastica van Gieson staining for elastin, and picrosirius red (PSR) for collagen. After rinsing with PBS, the sections were preincubated with 10% normal goat serum (Nichirei Biosciences, Tokyo, Japan) and incubated overnight at 4°C with mouse anti-CD68 and rabbit anti-MMP-9. Immunoreactivity was visualized using Alexa Fluor 488-conjugated anti-rabbit immunoglobulin G (Molecular Probes, Invitrogen, Carlsbad, CA, USA) and Alexa Fluor 594-conjugated anti-mouse immunoglobulin G. All Alexa Fluor-conjugated secondary antibodies were diluted 200-fold. The slides were mounted in a glycerol-based Vectashield medium (Vector Laboratories, Burlingame, CA, USA) containing the nucleus-staining reagent 4’,6-diamidino-2-phenylindole.
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