Ssc dc58 ap camera

Compounds were evaluated for their anticancer activity using three cultured cell lines: SNB-19 (human glioblastoma, DSMZ – German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany), C-32 (human amelanotic melanoma, ATCC-American Type Culture Collection, Manassas, VA), MDA-MB-231 (human adenocarcinoma mammary gland, ATCC, Manassas, VA) and HFF-1 (human fibroblast cell line, ATCC, Manassas, VA). The cultured cells were kept at 37 °C and 5% CO₂. The cells were seeded (1 × 10⁴ cells/well/100 µl DMEM supplemented with 10% FCS and streptomycin and penicillin) using 96-well plates (Corning). The cells were counted in a haemocytometer (Burker’s chamber) using a phase contrast Olympus IX50 microscope equipped with Sony SSC-DC58 AP camera and Olympus DP10 digital camera.

Compounds were evaluated for their antiproliferative activity using NHDF (normal human dermal fibroblasts, ATCC, Manassas, VA, USA). The cultured cells were kept at 37 °C and 5% CO₂. The cells were seeded (1 × 10⁴ cells/well/100 μL DMEM supplemented with 10% FCS and streptomycin/penicillin) into 96-well plates (Corning Inc., Corning, NY, USA). Cells were counted using a hemocytometer (Burker chamber) and phase contrast Olympus IX50 microscope equipped with Sony SSC-DC58 AP camera and Olympus DP10 digital camera. The cell viability of the compounds was determined using the Cell Proliferation Reagent WST-1 assay (Roche Molecular Biochemicals, Mannheim, Germany). The examined cells were exposed to the tested compounds (1 mg/mL DMSO stock) for 72 h at various concentrations (0.1–100 μg/mL). The control was included in order to eliminate the DMSO effect at the concentration used. Cell cultures were incubated with WST-1 (10 μL) for 1 h. The absorbance of the samples was measured against a background control at 450 nm using a microplate reader with a reference wavelength at 600 nm. The obtained results are expressed as means of at least two independent experiments performed in triplicate. The values of IC₅₀ (compound concentration required to cause 50% inhibition) were calculated from the dose–response relationship with respect to control.

All dipyridothiazines with 1,2,3-triazole substituents (1–4)b–f were evaluated for their anticancer activity using four cultured cell lines: SNB-19 (human glioblastoma, DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany), Caco-2 (human colon adenocarcinoma, DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany), A549 (human lung carcinoma, ATCC, Manassas, VA, USA) and MDA-MB231 (human breast adenocarcinoma, ATCC, Manassas, VA, USA), and NHDF (human dermal fibroblast cell line, ATCC, Manassas, VA, USA. The cultured cells were kept at 37 °C and 5% CO₂. The cells were seeded (1 × 10⁴ cells/well/100 µL DMEM supplemented with 10% FCS and streptomycin and penicillin) using 96-well plates (Corning). The cells were counted in a hemocytometer (Burker’s chamber) using a phase contrast Olympus IX50 microscope equipped with Sony SSC-DC58 AP camera and Olympus DP10 digital camera.