2 deoxyglucose 2 dg

Canagliflozin was provided by Mitsubishi Tanabe Pharma Corporation (Osaka, Japan). Streptozotocin and AICAR were purchased from Wako (Osaka, japan). Nicotinamide was purchased from SIGMA (St. Lewis, MO, USA). 2-Deoxyglucose (2-DG) was purchased from Tokyo Chemical Industries (Tokyo, Japan). Compound C was purchased from Calbiochem (CAS 866405-64-3). The cell culture reagents RPMI and DMEM were purchased from Nissui (Tokyo, Japan). Fetal bovine serum (FBS) was obtained from Biosera (Kansas City, MO, USA).
Antibodies were obtained from Cell Signaling Technology (pACC-11818S, ACC-3662S, pAMPK-4188S, AMPK-5832S, Cyclin D1-2922S, p-p70-9234S, p-70-2708S), R&D (PDGFRβ) and Santa Cruz (actin sc-47778 F1417, Pin-1sc46660 B0917).

Dulbecco’s modified Eagle’s medium (DMEM), insulin, dexamethasone, troglitazone, protease inhibitor cocktail III, and diamidinophenylindole (DAPI) were purchased from Wako, Osaka, Japan. Bovine serum (BS) and Alexa fluor 488-acetylated LDL were purchased from Life Technologies, Carlsbad, CA. DiI–VLDL was purchased from Kalen Biomedical, Germantown, MD. Blasticidin and fetal bovine serum (FBS) were purchased from Biowest, Nuaille, France. Isobutylmethylxanthine (IBMX), CO-releasing molecule 3 (CO-RM3), FLAG peptide, anti-FLAG antibody-conjugated agarose and [¹³C₆]-glucose were purchased from Sigma-Aldrich, St. Louis. 2-deoxyglucose (2-DG) was purchased from the Tokyo chemical industry, Tokyo, Japan.

Streptoglutarimide H (SGH, purity over 98%) was isolated and purified from a culture of marine-derived actinomycete Streptomyces sp. ZZ741 in rice medium [11 (link)]. Afatinib was obtained from Aladdin (Shanghai, China), cisplatin (DDP) from Hansoh Pharma (Jiangsu, China), 2-deoxyglucose (2-DG) from Tokyo Chemical Industry (Shanghai, China), oligomycin (OM) and MG132 from MedChemExpress (Princeton, NJ, USA), crystal violet and propidium iodide (PI) from Solarbio (Beijing, China), sulforhodamine B (SRB) and RNase A from Sigma-Aldrich (St. Louis, MO, USA). Glucose assay kit was purchased from Rongsheng (Shanghai, China), lactate assay kit from Jiancheng (Nanjing, China), ATP assay kit from Beyotime (Shanghai, China). All the antibodies used in this study are listed in Table S3 (Supplementary Material) .