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Sciex lipidyzer platform

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The Sciex Lipidyzer Platform is a laboratory instrument designed for comprehensive lipid analysis. It utilizes advanced mass spectrometry techniques to provide accurate and detailed identification and quantification of a wide range of lipid species from biological samples.

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Lab products found in correlation

Selexion tuning kit, by AB Sciex (3 mentions) Robovials, by Thermo Fisher Scientific (2 mentions) Lipidyzer internal standard mix, by AB Sciex (2 mentions) Gene chip mouse gene 430.2 arrays, by Thermo Fisher Scientific (1 mentions)

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Lipidyzer Platform (8 citations) Lipidyzer (8 citations)

5 protocols using sciex lipidyzer platform

1

Targeted Lipid Profiling from Plasma

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25 µl of plasma was pipetted into a glass tube for extraction. A modified Bligh and Dyer extraction (Bligh, 1959) is carried out on samples. Prior to biphasic extraction, a 13-lipid subclass Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers are dried down in a Genevac EZ-2 Elite. Lipid samples are resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis. Samples are analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 subclasses. Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list available upon request. Data analysis performed on Lipidyzer software. Quantitative values were normalized to volume. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of lipids.
Middlekauff H.R., William K.J., Su B., Haptonstall K., Araujo J.A., Wu X., Kim J, & Sallam T. (2020). Changes in lipid composition associated with electronic cigarette use. Journal of Translational Medicine, 18, 379.
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2

Lipidomic Analysis of DRG Cultures

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Primary DRG cultures were performed as described above. Cells were treated with vehicle or 15µM GW3965, with 5 biological replicates per treatment. Lipidomics was performed by UCLA Lipidomics Core using modified Bligh and Dyer lipid extraction for lipidomics analysis using The Sciex Lipidyzer Mass Spectrometry Platform. Samples were analyzed on the Sciex Lipidyzer Platform for quantitative measurement of 1100 lipid species across 13 lipid sub-classes. Data analysis was performed on Lipidyzer software. Quantitative values were normalized to the number of cells in the provided sample using Gene-Chip Mouse Gene 430.2 Arrays (Affymetrix, Santa Clara, CA).
Elshareif N., Gavini C.K, & Mansuy-Aubert V. (2022). LXR agonist modifies neuronal lipid homeostasis and decreases PGD2 in the dorsal root ganglia in western diet-fed mice. Scientific Reports, 12, 10754.
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3

Targeted Lipidomic Analysis of Plasma

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Lipids from 25 ul of plasma were extracted using a modified Bligh and Dyer extraction method by the UCLA lipidomics core facility56 . Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite evaporator. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10mM ammonium acetate and transferred to robovials (Thermo 10800107) for analysis. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 classes. Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list are available upon request.
Fung T.C., Vuong H.E., Luna C.D., Pronovost G.N., Aleksandrova A.A., Riley N.G., Vavilina A., McGinn J., Rendon T., Forrest L.R, & Hsiao E.Y. (2019). Intestinal serotonin and fluoxetine exposure modulate bacterial colonization in the gut. Nature microbiology, 4(12), 2064-2073.
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4

Targeted Lipidomic Analysis of Plasma

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Lipids from 25 ul of plasma were extracted using a modified Bligh and Dyer extraction method by the UCLA lipidomics core facility56 . Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite evaporator. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10mM ammonium acetate and transferred to robovials (Thermo 10800107) for analysis. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 classes. Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Instrument settings, tuning settings, and MRM list are available upon request.
Fung T.C., Vuong H.E., Luna C.D., Pronovost G.N., Aleksandrova A.A., Riley N.G., Vavilina A., McGinn J., Rendon T., Forrest L.R, & Hsiao E.Y. (2019). Intestinal serotonin and fluoxetine exposure modulate bacterial colonization in the gut. Nature microbiology, 4(12), 2064-2073.
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5

Shotgun Lipidomics of Liver Lipids

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These measurements were performed at the UCLA Lipidomics core. Briefly, a modification of the Bligh and Dyer protocol was used to extract lipids from frozen livers as previously published [11 ]. Prior to extraction, an internal standard mixture was added to each sample (AB Sciex 5040156, Avanti 330827, Avanti 330830, Avanti 330828, Avanti 791642). These lipid extracts that included the standards were quantitatively analyzed by Shot Gun Lipidomics, using the Sciex Lipidyzer Platform. The Differential Mobility Device on Lipidyzer was tuned with EquiSPLASH LIPIDOMIX (Avanti 330731). An in-house data analysis platform similar to the Lipidyzer Workflow Manager was used. The total non-esterified or free fatty acids and triglyceride quantitative values were normalized to mg of liver.
Gutierrez V., Kim-Vasquez D., Shum M., Yang Q., Dikeman D., Louie S.G., Shirihai O.S., Tsukamoto H, & Liesa M. (2024). The mitochondrial biliverdin exporter ABCB10 in hepatocytes mitigates neutrophilic inflammation in alcoholic hepatitis. Redox Biology, 70, 103052.
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