Agilent 5975c inert msd

The Tenax traps were analysed for volatile compounds with an Agilent 7890A gas chromatograph connected to an Agilent 5975C inert MSD with Triple Axis detector mass spectrometer (Agilent, Santa Clara CA, United States). The GC system was fitted with GERSTEL Multi-Purpose Sampler (MPS: Gerstel GmbH & Co. KG, Mülheim an der Ruhr, Germany). The GC capillary column (30 m, 250 µm internal diameter and 0.25 µm film thickness) was Agilent’s HP-5MS (5% phenyl and 95% dimethyl polysiloxane). Prior to analysis one microliter heptyl actetate (3.16 ng / µl) was added to the Tenax trap in a GERSTEL thermal desorption unit (TDU) followed by thermal desorption of the trap in splitless mode at initial temperature 40 °C and then increased by 120 °C / min to 270 °C, this end temperature was held for 5 min. The desorbed volatiles were focused on a tenax liner in a GERSTEL CIS inlet at 10 °C. The CIS inlet, which operated in splitless mode, was then heated at a rate of 12 °C/s to 280 °C during which the volatiles were transferred to the column. The GC oven temperature was initially held at 40 °C for 1 min and then increased by 4 °C / min to 280 °C which was held for 3 min. The carrier gas was helium at a pressure of 34 psi. The MS was at full scan and identified mass ranges from 30–400 m/z with electron ionization at 70 eV and ion source temperature at 230 °C.