Ampholites

All solvents used were of liquid chromatography-mass spectrometry (LC-MS) grade from Sigma-Aldrich (St. Louis, MO, USA) and Carlo Erba (Milan, Italy). Immobilized pH-gradient (IPG) and ampholites were purchased from Bio-Rad (Hercules, CA, USA). 3,5-T2, 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS), dithiothreitol (DTT), tosyl-phenylalanyl chloromethyl ketone-treated porcine trypsin, and other reagents for electrophoresis under native and denaturant conditions, or histochemical staining of respiratory complex activity were from Sigma-Aldrich. µZipTip C18 devices were from Millipore (Bedford, MA, USA).

3,5-diiodo-L-thyronine (T2) was purchased from Sigma-Aldrich Corp. (St. Louis, MO). All solvents used were of high-performance liquid chromatography-mass spectrometry (LC-MS) grade (Sigma-Aldrich, St. Louis, MO, USA and Carlo Erba, Milan, Italy). Immobilized pH-gradient (IPG) and ampholites were purchased from Bio-Rad Laboratories, Hercules, CA. Acrylamide, other reagents for polyAcrylamide gel preparation, BN-PAGE, and histochemical staining of respiratory complex activity, as well as CHAPS, urea, thiourea, dithioerythriol, EDTA, iodoacetamide, brilliant blue G-colloidal concentrate, and tosyl-phenylalanyl chloromethyl ketone (TPCK)-treated porcine trypsin were from Sigma-Aldrich. ZipTip C18 micro columns were from Millipore, Bedford, MA, USA.

Reagents were purchased from the following sources: middle range molecular weight protein markers, anti-mouse IgG horseradish peroxidase conjugate, 5-bromo-4-chloro-3 indolyl phosphate (BCIP), nitro blue tetrazolium (NBT), Promega; anti-rabbit IgG alkaline phosphatase conjugate, anti-bovine IgG horseradish peroxidase conjugate, anti-bovine IgG alkaline phosphatase conjugate, anti-equine IgG alkaline phosphatase conjugate, anti-mouse IgG alkaline phosphatase conjugate, diaminobenzidine (DAB), horseradish peroxidase type VI-A, fibrous DEAE-cellulose, benzamidine, iodoacetamide, phenyl methyl sulfonyl fluoride (PMSF), N-N′-1,2 phenylenedimaleimide (o-PDM), N-N′-1,4 phenyllenedimaleimide (p-PDM), gel filtration molecular weight protein marker kit, Staphylococcus aureus V8 protease, concanavalin A (Con A), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), methyl-α-d-mannopyranoside, methyl-α-d-glucopyranoside, Sigma; Q-Sepharose, S-Sepharose, Sefacryl S-300, Pharmacia; pre-stained high molecular weight protein markers, Gibco BRL; sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC), nitrocellulose (0.45 μm pore size), Pierce; broad range isoelectric focusing calibration kit (3–10), Immobilin dry strips (pH 5–8), ampholites (pH 3–10), BioRad. All other chemicals were of the highest quality grade available.