Mouse anti rab7

Antibodies used for immunofluorescence microscopy or western blotting were obtained from commercial sources. The following antibodies were used: rabbit anti-NHE6 (Abcam; 137185), rabbit anti-NHE9 (Abcam; 167157), mouse anti-RACK1 (BD Biosciences; 610177), rabbit anti-RACK1 (Cell Signaling Technology; 5432), mouse anti-tubulin (Sigma-Aldrich; T6199), rabbit anti-actin (Sigma-Aldrich; A5060), mouse monoclonal anti-HA (BioLegend; MMS-101P), mouse anti-EEA1(Santa Cruz Biotechnology; 6415), mouse anti-Rab7 (Santa Cruz Biotechnology; 10767), rabbit phospho-(Ser) PKC substrate (Cell Signaling Technology; 2261), mouse monoclonal PKC (Santa Cruz Biotechnology; 80), mouse monoclonal anti-LAMP2 (Abcam; 25631), rabbit anti-p-glycoprotein (Abcam; 129450). All Alexa Fluor secondary antibodies were acquired from Thermo Fisher Scientific. 4′, 6-diamidino-2-phenylindol dilactate (DAPI), Alexa Fluor-conjugated Tfn and Lysotracker were purchased from Thermo Fisher Scientific. Chemotherapeutic drugs (Dox, Mtx, Dau) were obtained from the local drug dispensary of the Centre Hospitalier Universitaire de Sherbrooke. Cq and Baf were obtained from Sigma-Aldrich. Cq was dissolved in water and Baf in dimethylsulfoxide. Control short hairpin RNA (shRNA) and shRNA directed against human NHE6 or NHE9 were purchased from Sigma-Aldrich. RNA interference directed against RACK1 was purchased from Ambion.

Leishmania expression vector (pXG-GFP+) was a generous gift from Dr Stephen M. Beverley (Washington University Medical School).
Following are the antibodies used for experiments: rabbit anti-GFP (catalog no.: BB-AB0065; BioBharati), mouse anti-GP63 (catalog no.: MA1-81830; Invitrogen), rabbit anti-ATP7A (catalog no.: ab125137; Abcam), goat anti-Rab11 (catalog no.: sc-6565; Santa Cruz Biotechnology), mouse anti-Rab7 (catalog no.: sc-376362; Santa Cruz Biotechnology), mouse anti-Lamp1 (Developmental Studies Hybridoma Bank: catalog no.: H4A3), mouse anti-Lamp2 (Developmental Studies Hybridoma Bank: catalog no.: H4B4), donkey anti-rabbit IgG (H + L) Alexa Fluor 488 (catalog no.: A-21206; Invitrogen), donkey antigoat IgG (H + L) Alexa Fluor 568 (catalog no.: A-11057; Invitrogen), and donkey antimouse IgG (H + L) Alexa Fluor 568 (catalog no.: A10037; Invitrogen). FM4-64FX dye (catalog no.: F34653; Invitrogen) was a generous gift from Dr Bidisha Sinha (Indian institute of Science Education and Research [IISER]).