Vectors for the overexpression of NEAT1 (pcDNA-NEAT1) and FLT1 (pcDNA-FLT1), the miR-373 mimic/inhibitor and their respective negative controls (pcDNA, NC mimic and NC inhibitor) were purchased from RiboBio. Small interfering RNAs (siRNAs) targeting NEAT1 and FLT1 (si-NEAT1, si-FLT1) and control si-NC were obtained from GenePharma (Shanghai, China). Trophoblast cells were transfected with these vectors using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
Pcdna neat1
Manufactured by RiboBio
Sourced in China
PcDNA-NEAT1 is a plasmid vector that contains the NEAT1 gene sequence. NEAT1 is a long non-coding RNA that plays a role in nuclear architecture and gene expression regulation. The plasmid can be used for the expression and study of the NEAT1 gene in cell-based experiments.
Automatically generated - may contain errors
Lab products found in correlation
Si neat1, by GenePharma (2 mentions)
Control si nc, by GenePharma (1 mentions)
Inhibitor nc, by RiboBio (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Si control, by GenePharma (1 mentions)
Mir 370 3p, by GenePharma (1 mentions)
Mir nc, by GenePharma (1 mentions)
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions)
Anti mir 370 3p, by GenePharma (1 mentions)
Pcdna control, by RiboBio (1 mentions)
Anti mir nc, by GenePharma (1 mentions)
Anti nc, by RiboBio (1 mentions)
Pcdna nc, by RiboBio (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Si neat1, by RiboBio (1 mentions)
3 protocols using pcdna neat1
1
Overexpression and Silencing of NEAT1 and FLT1
2
NEAT1, miR-370-3p Regulation Protocol
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si-RNA against NEAT1 (si-NEAT1), miR-370-3p mimic (miR-370-3p) and miR-370-3p inhibitor (anti-miR-370-3p), as well as the corresponding controls (si-control, miR-NC, anti-miR-NC), were obtained from GenePharma (Shanghai, China). NEAT1 overexpression plasmid (pcDNA-NEAT1), Irak2 overexpression plasmid (named as pcDNA-Irak2) and matched control (pcDNA-control) were acquired from RiboBio (Guangzhou, China). Cell transfection was performed using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA) following the standard manufacturer's protocol.
3
Modulating NEAT1 and miR-22-3p in Septic AKI
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Small interference RNA (siRNA) for NEAT1 (si-NEAT1) and the control siRNA (si-NC),
miR-22-3p mimic (miR-22-3p) and the control mimic (NC), miR-22-3p inhibitor
(anti-miR-22-3p) and the control inhibitor (anti-NC), NEAT1 overexpression vector
(pcDNA-NEAT1), and the empty vector (pcDNA-NC) were purchased from RiboBio
(Guangzhou, China). All plasmids and oligonucleotides were transfected into an
LPS-induced septic AKI model using Lipofectamine 2000 reagent (Invitrogen, Carlsbad,
CA, USA).
miR-22-3p mimic (miR-22-3p) and the control mimic (NC), miR-22-3p inhibitor
(anti-miR-22-3p) and the control inhibitor (anti-NC), NEAT1 overexpression vector
(pcDNA-NEAT1), and the empty vector (pcDNA-NC) were purchased from RiboBio
(Guangzhou, China). All plasmids and oligonucleotides were transfected into an
LPS-induced septic AKI model using Lipofectamine 2000 reagent (Invitrogen, Carlsbad,
CA, USA).
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