Beckman cx 7 biochemical autoanalyzer

All blood tests were performed after overnight fasting for at least 10 h in the hospital. Plasma samples for glucose and insulin detection were obtained at 0, 30, 60, 120 and 180 min after glucose loading, consistence with the 75-gram oral glucose tolerance test (OGTT). Plasma glucose concentrations and serum concentrations of triglycerides, total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol were measured using an autoanalyzer (Beckman CX-7 Biochemical Autoanalyzer, Beckman Coulter, Brea, CA). Serum insulin was measured using an electrochemiluminescence assay (Roche Diagnostics, Basel, Switzerland). The level of hemoglobin A1c (HbA1c) was determined using the high-performance liquid chromatography method (VARIANT™ II and D-10™ Systems, BIO-RAD, Hercules, CA, USA). Serum aldosterone and plasma renin activity were measured by radioimmunoassay (RIA) following the manufacturer’s instructions (A Beckman Coulter Corp). Serum cortisol and serum ACTH were measured by immunoluminescence and RIA following the manufacturer’s instructions (A Beckman Coulter Corp.). All tests were performed in the College of American Pathologists (CAP)-accredited laboratory (No. 7217913).

Blood samples were collected between 8:00 and 10:00 a.m. after an overnight fast (>8 h). These were then centrifuged for 20 min (2,000–3,000 rpm), and the supernatant was carefully collected (28 (link)). Total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-c), Ca, and P were detected using a biochemical autoanalyzer (Beckman CX-7 Biochemical Autoanalyzer; Beckman Coulter, Brea, CA, USA), and the 25(OH)D levels were assessed by DiaSorin radioimmunoassay (DiaSorin Inc., Stillwater, MN, USA). Intact parathyroid hormone (PTH) and β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX), procollagen type 1 N-terminal propeptide (P1NP), and osteocalcin (OC) were measured using an automated Roche electrochemiluminescence system (Roche Diagnostic GmbH, Mannheim, Germany). Tartrate-resistant acid phosphatase 5b (TRAP-5b) was measured using the tartrate resistant acid phosphatase assay kit (Beyotime, Shanghai, China).