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Luminex xponent

Manufactured by Thermo Fisher Scientific
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The Luminex xPONENT is a software platform that controls the Luminex instrument hardware and provides data analysis capabilities. It enables the detection and quantification of multiple analytes in a single sample using flow cytometry technology.

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Lab products found in correlation

Cytokine monkey magnetic 29 plex panel, by Thermo Fisher Scientific (2 mentions) Luminex 200, by Thermo Fisher Scientific (2 mentions) Luminex 200 instrument system, by Thermo Fisher Scientific (1 mentions) Bio plex pro human cytokine screening panel, by Bio-Rad (1 mentions)

3 protocols using luminex xponent

1

Profiling Maternal-Fetal Cytokine Dynamics

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To characterize the cytokine profiles of maternal plasma, placenta, neonatal umbilical cord, cord blood and amniotic fluid specimens, the Luminex assay was performed using the Bio‐Plex Pro Human Cytokine Screening Panel (Bio‐Rad Laboratories, Hercules, CA, USA), which can detect 48 cytokines and chemokines. The data were collected on a Luminex 200 Instrument System and analysed using Luminex xPONENT (Thermo Fisher Scientific, Waltham, MA, USA). The mean cytokine level in uninfected controls was considered the baseline and the cytokine levels in the specimens were expressed relative to the baseline level and termed "relative secretion levels". For negative controls, we used maternal plasma, placenta, umbilical cord, cord blood and amniotic fluid donated by three, four, two, two and three uninfected pregnant women at delivery respectively.
Wu H., Liao S., Wang Y., Guo M., Lin X., Wu J., Wang R., Lv D., Wu D., He M., Hu B., Long R., Peng J., Yang H., Yin H., Wang X., Huang Z., Lan K., Zhou Y., Zhang W., Xiao Z., Zhao Y., Deng D, & Wang H. (2021). Molecular evidence suggesting the persistence of residual SARS‐CoV‐2 and immune responses in the placentas of pregnant patients recovered from COVID‐19. Cell Proliferation, 54(9), e13091.
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2

Evaluating ZIKV Infection in Rhesus Monkeys

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Rhesus monkeys (Macacamulatta) were administered with EtOH or 25HC intravenously (i.v.) at 1.5 mg/kg of body weight 24 hr pre- or 4 hr post- intramuscular (i.m.) infection with 1×105 or 1×103 PFU of ZIKV GZ01 strain. They were subsequently treated with 25HC or EtOH daily for 7 days, and their body temperature and clinical signs were monitored daily. The blood and urine samples were collected from 0–7 dpi for viral load analysis by qRT-PCR. The serum collected at 0, 3, and 7 dpi were subjected to cytokine analysis with Monkey Cytokine Magnetic 29-Plex Panel (Thermo Fisher, LPC0005M) according to the manufacturer’s instructions. The data were collected on Luminex200 and analyzed by Luminex xPONENT (Thermo Fisher).
Li C., Deng Y.Q., Wang S., Ma F., Aliyari R., Huang X.Y., Zhang N.N., Watanabe M., Dong H.L., Liu P., Li X.F., Ye Q., Tian M., Hong S., Fan J., Zhao H., Li L., Vishlaghi N., Buth J.E., Au C., Liu Y., Lu N., Du P., Qin F.X., Zhang B., Gong D., Dai X., Sun R., Novitch B.G., Xu Z., Qin C.F, & Cheng G. (2017). 25-Hydroxycholesterol Protects Host against Zika Virus Infection and Its Associated Microcephaly in a Mouse Model. Immunity, 46(3), 446-456.
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3

Cytokine Profiling of Monkey Sera

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Monkey sera collected at different time points were subjected for cytokine, chemokines, and growth factors analysis with the Monkey Cytokine Magnetic 29-Plex Panel (Thermo Fisher, LPC0005M, USA) according to the manufacturer’s instruction. Each serum sample was measured with three replicates. The data were collected on Luminex200 and analyzed by Luminex xPONENT (Thermo Fisher, USA).
Deng Y.Q., Zhang N.N., Li X.F., Wang Y.Q., Tian M., Qiu Y.F., Fan J.W., Hao J.N., Huang X.Y., Dong H.L., Fan H., Wang Y.G., Zhang F.C., Tong Y.G., Xu Z, & Qin C.F. (2017). Intranasal infection and contact transmission of Zika virus in guinea pigs. Nature Communications, 8, 1648.
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