Serum was analysed from 19 RA patients (age 62.1±13.7, 21.1% male, 78.9% female; clinical and serological characteristics are described in Table 1 ) and age- and sex-matched OA patients (n = 30; age 61.8±8.1, 20% male, 80% female) and normal controls (n = 30 surgical injury repair patients; age 61.0±7.8, 20% male, 80% female). Synovial fluid from a separate cohort of 8 RA patients and 7 non-inflammatory OA patients was digested with 2 mg/ml hyaluronidase prior to assay [23] (link). ANGPTL4 (R&D Systems), C-reactive protein (CRP) (Invitrogen) and RANKL (Peprotech) were assayed according to the manufacturer’s instructions.
C reactive protein (crp)
Manufactured by Thermo Fisher Scientific
Sourced in United States
C-reactive protein (CRP) is a laboratory test used to measure the level of CRP in the blood. CRP is a protein produced by the liver that increases in response to inflammation in the body. The CRP test can be used to detect and monitor various inflammatory conditions.
Automatically generated - may contain errors
Lab products found in correlation
I fabp, by Hycult Biotech (4 mentions)
Soluble tissue factor, by R&D Systems (2 mentions)
Eotaxin, by Thermo Fisher Scientific (2 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (2 mentions)
Ip 10, by Thermo Fisher Scientific (2 mentions)
Luminex bio plex 200 system, by Bio-Rad (2 mentions)
Scd14, by R&D Systems (2 mentions)
Angptl4, by R&D Systems (1 mentions)
Rankl, by Thermo Fisher Scientific (1 mentions)
Pai 1, by Hyphen Biomed (1 mentions)
Scd163, by R&D Systems (1 mentions)
Cx3cl, by R&D Systems (1 mentions)
Cytometric bead array, by BD (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Ifn γ, by BD (1 mentions)
Fgf21, by R&D Systems (1 mentions)
Tbars assay kit, by ZeptoMetrix (1 mentions)
Adiponectin, by Crystal Chem (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Il 18, by Thermo Fisher Scientific (1 mentions)
15 protocols using c reactive protein (crp)
1
Serum Biomarkers in Rheumatoid Arthritis
2
Plasma Biomarker Analysis Protocol
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Blood samples were collected and centrifuged at 1500× g and 4 °C for 15 min for plasma separation. Plasma samples were collected to analyze the aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine (Cr), uric acid, and phosphorus concentrations by using a Roche Modular P800 Autoanalyzer (Diagnostics Roche, Basel, Switzerland). ACE activity was analyzed according to the method previously described by Vermeirssen et al. [15 (link)]. C-reactive protein (CRP; Invitrogen, CA, USA), plasminogen activator inhibitor-1 (PAI-1; HYPHEN BioMed, Neuville-sur-Oise, France), and endothelin-1 (ET-1; Enzo Life Sciences, New York, USA) were analyzed using commercial kits as per the manufacturer’s instructions.
3
Thrombotic and Bleeding Event Biomarkers
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From stored samples, patient plasma samples were selected as those nearest the time following the thrombotic or bleeding event (3 weeks on average; range 3 days to 5 weeks), as well as a sample taken 1-3 months prior to the event on the same patient.
All samples were analyzed with commercially available ELISA kits for total protein S (Diagnostica Stago, Parsippany, NJ), free protein S (Stago), protein C (Stago), soluble thrombomodulin (sTM; R&D Systems, Minneapolis, MN), soluble endothelial cell protein C receptor (sEPCR; Stago), and C-reactive protein (CRP; eBioscience, San Diego, CA). Results are given as % of normal or gravimetric quantity according to the calibrators in each assay kit.
All samples were analyzed with commercially available ELISA kits for total protein S (Diagnostica Stago, Parsippany, NJ), free protein S (Stago), protein C (Stago), soluble thrombomodulin (sTM; R&D Systems, Minneapolis, MN), soluble endothelial cell protein C receptor (sEPCR; Stago), and C-reactive protein (CRP; eBioscience, San Diego, CA). Results are given as % of normal or gravimetric quantity according to the calibrators in each assay kit.
4
Multiplex Biomarker Profiling in Plasma
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Concentrations of IL-1β, IL-1Ra, IL-6, IL-8, IL-10, IL-12p40, IL-12p70, IL-15, IL-17, IL-18, CCL2, CCL3, CCL4, CCL5, CCL11, CXCL10, IFN-γ, TNF-α, TGF-β, platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) (Bio-Plex, Bio-Rad, Hercules, CA), C-reactive protein (CRP) (eBioscience, San Diego, CA), sCD163, soluble tissue factor (sTF) (R&D Systems, Minneapolis, MN) and intestinal fatty acid binding protein (I-FABP) (Hycult Biotech, The Netherlands) were assessed in cryopreserved plasma samples maintained at −80 °C.
5
Comprehensive Cytokine Profiling in Biological Samples
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Concentrations of C-reactive protein (CRP) (eBioscience, San Diego, CA), Eotaxin, Fibroblast growth factor (FGF) – basic, FMS-like tyrosine kinase 3 ligand (FLT3L), Granulocyte colony-stimulating factor (G-CSF), intestinal fatty acid binding protein (I-FABP) (Hycult Biotech, The Netherlands), Interferon (IFN)-α, IFN-β, IFN-γ, Interleukin (IL)-1Ra, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17, Interferon-γ-induced protein 10 (IP-10), Monocyte chemoattractant protein (MCP)-1, Macrophage inflammatory protein (MIP)-1, MIP-1α, MIP-1β, Platelet-derived growth factor (PDGF), Regulated on activation normal T cell expressed and secreted (RANTES), soluble CD14 (sCD14), soluble CD163 (sCD163), soluble GzB, soluble Programmed cell death protein (PD) -1, soluble Tissue factor (sTF), Transforming Growth Factor (TGF)-β, Tumor Necrosis Factor (TNF)-α, and vascular endothelial growth factor (VEGF) were assessed using Luminex assay technology (Bio-Plex, Bio-Rad, Hercules, CA) were assessed in cryopreserved plasma samples maintained at −80°C.
6
Plasma Biomarkers in Infectious Diseases
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Concentrations of IL-1β, IL-1Ra, IL-6, IL-8, IL-10, IL-12p40, IL-12p70, IL-15, CCL2, CCL3, CCL4, CCL5, CCL11, CXCL10, IFN-γ, TNF-α, TGF-β, platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF) (Bio-Plex, Bio-Rad, Hercules, CA), C-reactive protein (CRP) (eBioscience, San Diego, CA), soluble (s) CD14, sCD163, soluble tissue factor (sTF) (R&D Systems, Minneapolis, MN) and intestinal fatty acid binding protein (I-FABP) (Hycult Biotech, The Netherlands) were assessed in cryopreserved plasma samples maintained at −80°C from patients in the Indian cohort. A smaller panel of biomarkers containing IL-6, IL-8, IL-10, IL-12p40, IFN-γ, TNF-α, CCL3, CCL4, CXCL10, sCD14, sCD163 and sTF was investigated in the South African cohort due to limitations in available plasma volume.
7
Multiplex Biomarker Profiling in Plasma
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Concentrations of C-reactive protein (CRP) (eBioscience, San Diego, CA), Eotaxin, Fibroblast growth factor (FGF) – basic, FMS-like tyrosine kinase 3 ligand (FLT3L), Granulocyte colony-stimulating factor (G-CSF), intestinal fatty acid binding protein (I-FABP) (Hycult Biotech, The Netherlands), Interferon (IFN) -α, IFN-β, IFN-γ, Interleukin (IL)- 1Ra, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17, Interferon-γ-induced protein 10 (IP-10/CXCL10), Monocyte chemoattractant protein-1 (MCP-1/CCL2), Macrophage inflammatory protein (MIP-1α/CCL3), MIP-1β (CCL4), Platelet-derived growth factor (PDGF), Regulated on activation normal T cell expressed and secreted (RANTES/CCL5), soluble CD14 (sCD14), soluble CD163 (sCD163), soluble Granzyme B (sGzB), soluble Programmed Cell death protein (PD) -1, soluble Tissue factor (sTF), Transforming Growth Factor (TGF)-β, Tumor Necrosis Factor (TNF)-α, and vascular endothelial growth factor (VEGF) (Bio-Plex, Bio-Rad, Hercules, CA)were assessed in cryopreserved plasma samples maintained at −80 °C.
8
Serum Collection and CRP/Ferritin Quantification
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Serum samples were collected at the indicated study timepoints using vacuum blood collection tubes (BD Vacuntainer®, Becton Dickinson, Franklin Lakes, NJ). Serum was separated by centrifugation following the manufacturer’s instructions. Samples were aliquoted in cryotubes and stored at -80°C until use in the immunoassays. Serum levels of CRP (Ebioscience, San Diego, CA) and ferritin (Abnova, Taipei, Taiwan) were determined using an ELISA-based assay following the manufacturers’ protocols.
9
Fasting Plasma Leptin and CRP Analysis
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The participants were instructed to fast for at least 9 h prior to each examination. Blood samples for the leptin and CRP assays were collected between 08:00 and 10:00 am in 5-mL EDTA tubes and stored at 4 °C in a fridge. Plasma was isolated by centrifugation at 1800×g for 15 min at 4 °C and immediately stored at − 80 °C. The levels of fasting plasma leptin (Linco Research, St Louis, MO, USA) and CRP (eBioscience, San Diego, CA, USA) were measured using ELISA methods. For detailed information on the catalog numbers, minimum detectable levels, and intra- and inter-assay coefficients of the ELISA kits used for the measurement of leptin and CRP, please see Supplemental Table S5 . Dilute hs-CRP samples from 1:30 to 1:1000 with Assay Buffer.
10
Multiplex Cytokine Profiling Protocol
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IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, IL-1α, IL-1β, and MCP-1 were analyzed by Cytometric Bead Arrays (BD Biosciences) in accordance with the manufacturer's instructions. ELISA kits were used for TNF-α (PeproTech, Rocky Hill, New Jersey, USA), IFN-γ (BD Biosciences), CRP (Invitrogen, ThermoFisher Scientific) and CX3CL (R&D systems).
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