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Ab61065

Manufactured by Abcam
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Sourced in Japan

Ab61065 is an antibody that recognizes Keratin 14. Keratin 14 is a type I intermediate filament protein that is expressed in basal cells of stratified squamous epithelia. This antibody is suitable for use in various applications such as immunohistochemistry, western blotting, and flow cytometry.

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Lab products found in correlation

Alexa 594, by Thermo Fisher Scientific (1 mentions) Lsm 800, by Zeiss (1 mentions) Vectashield hardset mounting medium with dapi, by Vector Laboratories (1 mentions) Ab2402, by Abcam (1 mentions) Ab2175, by Abcam (1 mentions) Ab63801, by Abcam (1 mentions) Ab79398, by Abcam (1 mentions) Cyclin a, by Santa Cruz Biotechnology (1 mentions) Pchk2 t68, by Cell Signaling Technology (1 mentions) Ab1791, by Abcam (1 mentions) Nb100 544, by Novus Biologicals (1 mentions) Ppten s380, by Cell Signaling Technology (1 mentions) Brca2, by Merck Group (1 mentions) Importin β, by Abcam (1 mentions) P chk1 s317, by Cell Signaling Technology (1 mentions) Bach1, by Santa Cruz Biotechnology (1 mentions) γh2ax, by Merck Group (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) γ tubulin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using ab61065

1

Immunofluorescence Assay for Phospho-RPA2

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Cells were seeded onto 8-well chamber slides and incubated for 1 day, followed by compound treatment for 2 h. The cells were fixed with 4% paraformaldehyde phosphate buffer and permeabilized with PBS, including 0.5% Triton(R) X-100. Permeabilized cells were incubated with the primary antibody for anti-phospho-RPA2 (Thr21) (#ab61065, Abcam), followed by Alexa 594-labeled secondary antibody (#A-11037, Thermo Fisher Scientific Inc.), and mounted in Vectashield HardSet Mounting Medium with DAPI (Vector Laboratories Inc.). Slides were examined using a confocal laser scanning microscope LSM800 (Carl Zeiss, Inc.).
Ueno H., Hoshino T., Yano W., Tsukioka S., Suzuki T., Hara S., Ogino Y., Chong K.T., Suzuki T., Tsuji S., Itadani H., Yamamiya I., Otsu Y., Ito S., Yonekura T., Terasaka M., Tanaka N, & Miyahara S. (2022). TAS1553, a small molecule subunit interaction inhibitor of ribonucleotide reductase, exhibits antitumor activity by causing DNA replication stress. Communications Biology, 5, 571.
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2

Comprehensive Antibody Panel for DNA Damage Response

Cited 1 time
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We used the following rabbit antibodies: TOPBP1 (ab2402; Abcam), RAD51 (sc8349; Santa Cruz; ab63801; Abcam), pRAD51 S14 (Yata et al., 2012 (link)), cyclin A (NCL-CYCLINA; Leica), RPA70 (ab79398; Abcam), pRPA32 T21 (ab61065; Abcam), pRPA32 S4/8 (A300-245A; Bethyl), pRPA32 S33 (NB100-544; Novus), pCHK1 S317 (2344; Cell Signaling), pCHK2 T68 (2661; Cell Signaling), H3 (ab1791; Abcam), pH3 S10 (06–570; Millipore), BACH1 (sc-28738; Santa Cruz), pPTEN S380 (9551; Cell Signaling), pLRRK1 S1790 (H. Hanafusa, Nagoya University, Nagoya, Japan; Hanafusa et al., 2015 (link)); mouse antibodies: γH2AX (05–636; Millipore), cyclin A (sc-751; Santa Cruz), BrdU (RPN20AB; AP Biotech), RPA32 (ab2175; Abcam), BRCA1 (sc-6954; Santa Cruz), BRCA2 (OP95; Millipore), CHK1 (sc-8408; Santa Cruz), Importin-β (ab2811; Abcam), Lamin A/C (sc-7292; Santa Cruz), α-tubulin (sc-8035; Santa Cruz), PLK1 (05–844; Millipore; 331700; Zymed); and goat antibody: γ-tubulin (sc-7396; Santa Cruz).
Moudry P., Watanabe K., Wolanin K.M., Bartkova J., Wassing I.E., Watanabe S., Strauss R., Troelsgaard Pedersen R., Oestergaard V.H., Lisby M., Andújar-Sánchez M., Maya-Mendoza A., Esashi F., Lukas J, & Bartek J. (2016). TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. The Journal of Cell Biology, 212(3), 281-288.
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