C57bl 6n mice

Wild-type C57Bl/6 N mice purchased from Taconic Biosciences or bred in-house were used for all experiments. The inducible mouse model of MLL-ENL-driven leukemia (iMLL-ENL; Col1a1^TetO_MLL-ENL) has been described previously [59 (link)]. All experiments involving animals were performed in accordance with ethical permits approved by the Swedish Board of Agriculture. Animals were housed in individually ventilated cages (IVC) and provided with sterile food and water ad libitum. Adult mice used in experiments were 10–20 weeks old. E14.5 embryos and neonatal mice were obtained by timed pregnancies overnight. The morning after mating was considered E0.5. For proteome analysis, equal numbers of male and female mice were used.

Female 6-week-old C57BL6/N mice were purchased from the indicated barrier rooms at Taconic Biosciences (Tac) (Hudson, NY) and allowed to acclimate for 1 week before collecting fecal pellets or cecal content and starting infections. Mice were kept on NIH-31 Modified Open Formula Mouse/Rat Irradiated Diet (Envigo item#; 7913, Huntingdon, UK) and autoclaved, non-acidified, reverse osmosis water. Mice were kept on a 12-h light/dark cycle from 6 AM to 6 PM and 6 PM to 6 AM, respectively. All animal handling and experimentation were reviewed and approved by the University of Louisville Institutional Animal Care and Use Committee based on the recommendations of the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. Mice were euthanized by carbon dioxide asphyxiation.

Wild-type (WT) C57BL/6N mice were purchased from Taconic (Hudson, NY). RAGE-knockout (RAGE^-/-) mice were a king gift from Dr. A. Bierhaus (University of Heidelburg, Heidelburg, Germany) and a breeding colony was established (39 (link)). Both male and female mice were used at eight to ten weeks of age in all animal experiments. All animal studies were carried out under the National Research Council’s guidelines in the ‘‘Guide for the care and use of laboratory animals,’’ with oversight by the University of Pittsburgh’s Institutional Animal Care and Use Committee.

Two strains of mice were used in these experiments. First, we used WT mice that were derived from a cross between 129Svev and C57Bl/6N mice (Taconic). Second, to suppress adult neurogenesis we used transgenic mice expressing a modified HSV-tk gene under control of the nestin promoter (TK⁺ mice)16 (link)34 (link). These mice were maintained on a C57Bl/6N background (>8 crosses), and experimental mice were generated by crossing TK⁺ mice with 129Svev WT mice. In all experiments, we used both male and female mice. Breeding was conducted in the Hospital for Sick Children animal facility. Mice were housed in standard laboratory conditions with 3–5 mice per cage and had free access to food and water. Rooms were maintained on a 12 h light/dark cycle and behavioural testing occurred during the light phase of the cycle. Experiments were conducted in accordance with the Canadian Council on Animal Care guidelines and the protocols were approved by the animal care committee at the Hospital for Sick Children.

Experiments were performed in male C57BL/6N mice (3 months old; Taconic, Tornbjerg, Denmark) and were carried out in accordance with the guidelines of Research Ethics Committee of Karolinska Institutet and Swedish Animal Welfare Agency. All efforts were made to minimize animal suffering and to reduce the number of animals used.