Groups (n=10–20) of 8-week-old Swiss albino CD1 male mice were intraperitoneally (i.p.) inoculated with 5 × 105 plaque-forming units (pfu)/mouse of the African, American and Asian ZIKV strains, or VSV, in 100 μL of Eagle’s minimal essential medium (EMEM) (BE12-125F, Lonza, Verviers, Belgium) as the vehicle. As a control, a group of CD1 mice was inoculated with vehicle alone. Mice were i.p. challenged with 104 pfu/mouse of a neurovirulent WNV NY99 strain 14 days after primary ZIKV or VSV infection. Viruses were back titrated to confirm inoculation doses. Animals were bled prior to infection, 5 and 13 days post-primary infection, and 5, 7 and 26 days post-WNV challenge (corresponding to 19, 21 and 40 days post-ZIKV infection, d.p.i.). Viral infections and sample collection were conducted as described.36 (link), 37 (link), 38 (link)During the experiments, all animals were monitored daily and received water and food ad libitum. Those mice showing signs of disease were anesthetized and killed, as were all surviving animals at the end of the experiment (40 days after ZIKV infection).
Be12 125f
Manufactured by Lonza
Sourced in Belgium
The BE12-125F is a laboratory equipment product manufactured by Lonza. It is designed for cell culture applications. The core function of this product is to provide a temperature-controlled environment for cell growth and maintenance.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using be12 125f
1
ZIKV Infection and WNV Challenge in Mice
2
Cytotoxicity Evaluation of NovioSense Glucose Sensor
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cytotoxicity tests were outsourced and conducted
by Toxikon Europe
NV, Belgium according to ISO 10993-5, 2009, Biological Evaluation
of Medical Devices; Part 5: Tests in vitro cytotoxicity and ISO 10993-12,
2012, Biological Evaluation of Medical Devices; Part 12: Sample Preparation
and Reference Materials. The study was conducted in accordance with
Good Laboratory Practice (GLP). The test was based on the measurement of
viability of L929 mouse fibroblasts (105 cells/mL) in response
to an extract of the NovioSense Glucose Sensor functionalized with
GOx dummy device via XTT assay (Roche). The NovioSense Glucose Sensor
devices were extracted in 0.9% sodium chloride at a ratio of 60 cm2/20 mL. The NovioSense Glucose Sensor device extract was finally
diluted in serum-supplemented Minimum Essential Medium (MEM-complete)
at a ratio of 1/4. The L929 cells were exposed in quadruplicate at
this 1/4 dilution. MEM-complete contained following: MEM (LONZA:BE12-125F),
penicillin/streptomycin (LONZA: DE17-602E), fetal bovine serum (Greiner-bio-one
FBSEU500),l -glutamine (LONZA:BE17-605E), Hepes buffer (LONZA:
BE17–737E).
by Toxikon Europe
NV, Belgium according to ISO 10993-5, 2009, Biological Evaluation
of Medical Devices; Part 5: Tests in vitro cytotoxicity and ISO 10993-12,
2012, Biological Evaluation of Medical Devices; Part 12: Sample Preparation
and Reference Materials. The study was conducted in accordance with
Good Laboratory Practice (GLP). The test was based on the measurement of
viability of L929 mouse fibroblasts (105 cells/mL) in response
to an extract of the NovioSense Glucose Sensor functionalized with
GOx dummy device via XTT assay (Roche). The NovioSense Glucose Sensor
devices were extracted in 0.9% sodium chloride at a ratio of 60 cm2/20 mL. The NovioSense Glucose Sensor device extract was finally
diluted in serum-supplemented Minimum Essential Medium (MEM-complete)
at a ratio of 1/4. The L929 cells were exposed in quadruplicate at
this 1/4 dilution. MEM-complete contained following: MEM (LONZA:BE12-125F),
penicillin/streptomycin (LONZA: DE17-602E), fetal bovine serum (Greiner-bio-one
FBSEU500),
BE17–737E).
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