2,4,6-TBP (99% pure), T4 (> 99%), T3 (> 99%), rT3 (> 99%), 3,3′-T2 (> 99%), and adenosine 3′-phosphate 5′-phosphosulfate (PAPS) lithium salt hydrate (> 60%) were purchased from Sigma-Aldrich (St. Louis, MO). BDE-47 (> 97%), BDE-99 (> 97%), 3-OH 2,2′,4,4′-BDE-47 (3-OH BDE-47; 99%), and 6-OH 2,2′,4,4′-BDE-47 (6-OH BDE-47; 100%) were purchased as neat standards from AccuStandard (New Haven, CT). [13C6]-3,3′-T2 was purchased from Isotec (Miamisburg, OH). 3,3′-T2 sulfate (3,3′-T2S; 98%) was custom synthesized by the Duke University Small Molecule Synthesis Facility (Durham, NC). T3 sulfate (T3S) was purchased from Toronto Research Chemicals (Toronto, ON; 98.6%).
Ham’s F-12K (Kaighn’s Modification) Medium cell culture media and other cell culture reagents were purchased from Life Technologies (Carlsbad, CA). All solvents and other reagents were purchased from VWR (Radnor, PA). BeWo cells (ATCC® CCL-98™) were obtained from the Duke University Cell Culture Facility (Durham, NC). All cells were grown in Tissue Culture treated flasks (75 and 182 cm2) and dishes (36.3 cm2) from Genesee Scientific (San Diego, CA).
Ham’s F-12K (Kaighn’s Modification) Medium cell culture media and other cell culture reagents were purchased from Life Technologies (Carlsbad, CA). All solvents and other reagents were purchased from VWR (Radnor, PA). BeWo cells (ATCC® CCL-98™) were obtained from the Duke University Cell Culture Facility (Durham, NC). All cells were grown in Tissue Culture treated flasks (75 and 182 cm2) and dishes (36.3 cm2) from Genesee Scientific (San Diego, CA).