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5 ump

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5′-UMP is a nucleotide molecule that serves as a building block for ribonucleic acid (RNA) synthesis. As a phosphorylated form of the nucleoside uridine, 5′-UMP plays a fundamental role in various biological processes.

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Lab products found in correlation

5 cmp, by Merck Group (1 mentions) Deuterated water d2o, by Merck Group (1 mentions) 5 gmp, by Merck Group (1 mentions) 5 amp, by Merck Group (1 mentions) Uracil, by Merck Group (1 mentions) Ribose, by Merck Group (1 mentions)

2 protocols using 5 ump

1

Nucleotide Characterization and Preparation

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The disodium salts of all four 5′-NMPs, viz. 5′-AMP, 5′-GMP, 5′-UMP, and 5′-CMP (all purity ≥ 98%), were purchased from Sigma-Aldrich (Bangalore, India) and used without any further purification. Analytical grade PEG 8000, dextran (average mol. wt. of 9,000- 11,000), and deuterated water (D2O) were also purchased from Sigma-Aldrich.
Bapat N.V., Paithankar H., Chugh J, & Rajamani S. (2020). NMR analysis of nucleotide π-stacking in prebiotically relevant crowded environment. Communications Chemistry, 3, 51.
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2

Picosecond Pulse Radiolysis of Nucleic Acid Components

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The chemical compounds (5′-UMP in the form of disodium salts, Urd, uracil and ribose; purity, >99%) were purchased from Sigma-Aldrich and were used without further purification. The 5 ps picosecond pulse radiolysis transient absorbance measurements were performed at the electron facility ELYSE29 (Université Paris Sud, France), coupled with transient absorption broadband probe spectroscopy (360–800 nm for timescales <10 ns, 250 – 850 nm for timescales >10 ns, see SI for details). The spectra of the absorbing species in the type of system studied here are known to strongly overlap; as a result, it becomes difficult to deconvolute individual spectra. Therefore, a global data analysis approach was used by using SK-Ana code.30
Ma J., Denisov S.A., Marignier J.L., Pernot P., Adhikary A., Seki S, & Mostafavi M. (2018). Ultrafast Electron Attachment and Hole Transfer Following Ionizing Radiation of Aqueous Uridine Monophosphate. The journal of physical chemistry letters, 9(17), 5105-5109.
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