Contractile testing was performed 14 days after injury using an in situ testing apparatus (Aurora, Model 809B, Aurora Scientific Inc., Canada), stimulator (Model 701C, Aurora Scientific Inc.) and force transducer (Aurora Scientific Inc.). Animals were anesthetized, the right Achilles tendon was cut to prevent plantar flexion, and the peroneal nerve was exposed via a small incision on the lateral aspect of the right knee. Mice were then placed supine on a platform with the foot to be tested positioned at 20° of plantarflexion and affixed to the footplate using cloth tape. Ankle dorsiflexors were stimulated six times at a single frequency (60 Hz) by placing a hook electrode on the peroneal nerve. Muscle peak twitch, time to peak twitch and half-relaxation time were quantified. Next, successive tetanic contractions at 10, 30, 50, 80, 100, 120, 150, 180, and 200 Hz were elicited to obtain a force-frequency curve, with a 2-minute rest between contractions, as per our previous reports (Clemens et al., 2021 ; Sahu et al., 2021 (link); Sahu et al., 2018 ; Zhang et al., 2016 (link)). Peak specific tetanic force was quantified by extracting the highest force value from the series of tetanic contractions of each mouse. Animals displaying evidence of external injuries and/or tumor growths were excluded from analysis.
Model 809b
Manufactured by Aurora Scientific
Sourced in Canada
The Model 809B is a high-precision force transducer designed for laboratory applications. It features a compact and durable construction, with a measurement range up to 2 Newtons. The device utilizes strain gauge technology to accurately detect and measure applied forces.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using model 809b
1
Contractile Function Assessment of Injured Muscle
2
Contractile Function Assessment of Injured Muscle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Contractile testing was performed 14 days after injury using an in situ testing apparatus (Aurora, Model 809B, Aurora Scientific Inc., Canada), stimulator (Model 701C, Aurora Scientific Inc.) and force transducer (Aurora Scientific Inc.). Animals were anesthetized, the right Achilles tendon was cut to prevent plantar flexion, and the peroneal nerve was exposed via a small incision on the lateral aspect of the right knee. Mice were then placed supine on a platform with the foot to be tested positioned at 20° of plantarflexion and affixed to the footplate using cloth tape. Ankle dorsiflexors were stimulated six times at a single frequency (60 Hz) by placing a hook electrode on the peroneal nerve. Muscle peak twitch, time to peak twitch and half-relaxation time were quantified. Next, successive tetanic contractions at 10, 30, 50, 80, 100, 120, 150, 180, and 200 Hz were elicited to obtain a force-frequency curve, with a 2-minute rest between contractions, as per our previous reports (Clemens et al., 2021 ; Sahu et al., 2021 (link); Sahu et al., 2018 ; Zhang et al., 2016 (link)). Peak specific tetanic force was quantified by extracting the highest force value from the series of tetanic contractions of each mouse. Animals displaying evidence of external injuries and/or tumor growths were excluded from analysis.
3
In Situ Muscle Contractility Measurements
4
In Situ Contractile Evaluation of Ischemic Muscle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Twenty one days following FAL, the force producing capacity of the ischemic TA muscle of control and experimental animals was evaluated using an in situ contractile testing apparatus (Model 809B, Aurora Scientific, Aurora, ON, Canada). Mice were anesthetized prior to surgery, and the peroneal nerve was exposed. The Achilles tendon was then severed in order to prevent any counteracting muscle response and the foot was secured to the footplate at a 20° angle. The electrodes were then placed on the exposed peroneal nerve after which the first single twitch stimulation protocol was conducted to measure peak twitch, time to peak twitch, and half-relaxation time. Next, the force-frequency curve was obtained by sequential administration of tetanic stimulations at 10, 30, 50, 80, 100, 120, 150, 180 and 200 Hz, with two-minute intervals between contractions. Results of both, single and tetanic stimulations were collected in torque (mN-m), and the absolute force values (mN) were evaluated by dividing torque by the length of the footplate (0.03 m). Specific force was then calculated by dividing the absolute force values by physiological cross-sectional area (CSA), calculated as the [muscle weight (mg)]/[muscle length (mm) × muscle density (1.06 mg/mm3)] (Egginton 1999 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!