About 0.05 mL of allantoic fluid of fertilized hen’s eggs, in which influenza A/Aichi/2/68 (H3N2) virus was propagated, was atomized inside the patient hood, simulating bioparticles of a patient’s cough using an electric compressor-type nebulizer, which generated mists of 1–10 µm diameter particles (NE-C16; Omron, Kyoto, Japan). Airborne particles were collected from inside and outside of the patient hood using an air sampler and a gelatin membrane filter (MD8 AirScan Sartorius AG, Göttingen, Germany) for virus detection and quantitation, as described previously (11 (link)). A total of 320 L of air was passed through the membrane at a flow rate of 80 L/min (i.e., 4 minutes sampling time). The gel membrane filter was then dissolved in 10 mL of culture medium, Minimal Essential Medium Eagle (Sigma-Aldrich, UK), (37 °C) then subjected to a conventional plaque assay using Madin-Darby canine kidney (MDCK) cells (12 (link)).
Ne c16
Manufactured by Omron
Sourced in Japan
The NE-C16 is a compact nebulizer from Omron, designed for the efficient delivery of aerosolized medication. It features an air compressor that generates the necessary airflow for nebulization. The device is intended for use in a clinical or home setting for the administration of prescribed inhalation therapies.
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Lab products found in correlation
3 protocols using ne c16
1
Aerosolized Influenza Virus Detection
2
Influenza Virus Propagation and Atomization
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Influenza virus A/Aichi/2/68 (H3N2) was propagated in the allantoic cavities of fertilized chicken eggs. The allantoic fluid containing the viruses was harvested and atomized in simulation experiments using an electric compressor nebulizer (NE-C16; Omron, Kyoto, Japan).
3
Quantification of Influenza A M1 Protein
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Three individual aerosols, each containing one of the microbes, were prepared. The test devices were set up in a biosafety cabinet (Fig. 1) , as described in a previous study (Shimasaki et al., 2016a) . The nebulizer (a custom-made glass or NE-C16, Omron Healthcare Co., Ltd., Kyoto, Japan) was fitted upstream of the airflow in the test duct, which contained the sample holder. The sample fabric was held in place in the sample holder. The test microbe suspension was placed ［A/Puerto Rico/8/34/Mount Sinai］ M1 protein) at 37℃ for 1.5 h. After washing with TBS-T, 100 µL of anti-flu A M1 goat polyclonal antibody was added to the wells, and the plate was incubated at 37℃ for 1 h. After washing with TBS-T, 100 µL of anti-goat IgG-HRP was added to the wells, and the plate was incubated at 37℃ for 1 h. The plate was then washed with TBS-T, and 100 µL of TMB-substrate was added. The reaction was stopped by adding 100 µL of TMB Stop Buffer, and the optical density at 450 nm was measured using a multiwell plate reader.
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