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Sabouraud dextrose agar

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Sabouraud dextrose agar is a microbiological growth medium used for the cultivation and isolation of fungi. It contains dextrose as a carbon source and peptone as a nitrogen source, providing the necessary nutrients for the growth of a wide range of fungal species.

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Lab products found in correlation

Malt agar extract, by Avantor (2 mentions) Vitek ms system, by bioMérieux (2 mentions) Calcofluor staining, by BD (2 mentions) Gentamicin and chloramphenicol, by Bio-Rad (2 mentions) Maldi tof microflex system, by Bruker (2 mentions) Mbt compass ivd 4.2 database, by bioMérieux (2 mentions) Vitek ms v1.6.0 database, by bioMérieux (2 mentions) Sc5314, by American Type Culture Collection (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) Chloramphenicol, by Bio-Rad (1 mentions) Cycloheximide (chx), by Bio-Rad (1 mentions)

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Sabouraud agar (2 citations)

6 protocols using sabouraud dextrose agar

1

Candida albicans Infection Protocol

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The strain of C. albicans used throughout these experiments was provided by ATCC (ATCC® MYA2876™), and was maintained on Sabouraud dextrose agar (SDA; Biorad, Hercules, CA, USA) plates containing gentamicin and chloramphenicol. Growth from an 18- to 24-h SDA culture of C. albicans was suspended in sterile saline solution (NaCl 0.9%) for mice administration or in culture medium for in vitro experiment [13 (link),29 (link)].
Lactobacillus helveticus LA401 and Lactobacillus gasseri LA806 were provided by Genibio (Lorp-Sentaraille, Paris, France). The combination of the two strains is marketed under the name Lactibiane Cnd (PiLeJe Laboratoire, Paris, France).
Authier H., Salon M., Rahabi M., Bertrand B., Blondeau C., Kuylle S., Holowacz S, & Coste A. (2021). Oral Administration of Lactobacillus helveticus LA401 and Lactobacillus gasseri LA806 Combination Attenuates Oesophageal and Gastrointestinal Candidiasis and Consequent Gut Inflammation in Mice. Journal of Fungi, 7(1), 57.
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2

Quantification of Candida albicans in Murine Stools

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The strain of C. albicans used throughout these experiments (sc-5314) was provided by the American Type Culture Collection (ATCC) and was maintained on Sabouraud dextrose agar (SDA; Bio-Rad, Hercules, CA, USA) plates containing gentamicin and chloramphenicol. Growth from an 18 to 24 h SDA culture of C. albicans was suspended in sterile saline solution (NaCl 0.9%) for mice infection (49 (link), 50 (link)).
Stools were collected daily from day 3 to day 5 after infection, weighed, and mechanically homogenized in phosphate buffer saline (PBS). Serial dilutions of homogenates were plated on SDA plates containing gentamicin and chloramphenicol for the quantitative determination of the number of C. albicans. Plates were incubated at 37°C for 24 h and the number of colonies was counted to determine the colonies forming unit (CFU)/g of stool.
Authier H., Bardot V., Berthomier L., Bertrand B., Blondeau C., Holowacz S, & Coste A. (2022). Synergistic Effects of Licorice Root and Walnut Leaf Extracts on Gastrointestinal Candidiasis, Inflammation and Gut Microbiota Composition in Mice. Microbiology Spectrum, 10(2), e02355-21.
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3

Candida albicans blood isolate protocol

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The strain of C. albicans used throughout these experiments was isolated from a blood culture of a patient in the Toulouse-Rangueil University Hospital (98/26135). The isolate was identified as C. albicans based on common laboratory criteria and cultured on Sabouraud dextrose agar (SDA; Biorad, Hercules, CA, USA) plates containing gentamicin and chloramphenicol. C. albicans was maintained by transfers on SDA plates. Growth from an 18- to 24-h SDA culture of C. albicans was suspended in sterile saline buffer (HBSS; Life Technologies). In all experiments, the h-MDMs were challenged with blastospores.
Benmoussa K., Authier H., Prat M., AlaEddine M., Lefèvre L., Rahabi M.C., Bernad J., Aubouy A., Bonnafé E., Leprince J., Pipy B., Treilhou M, & Coste A. (2017). P17, an Original Host Defense Peptide from Ant Venom, Promotes Antifungal Activities of Macrophages through the Induction of C-Type Lectin Receptors Dependent on LTB4-Mediated PPARγ Activation. Frontiers in Immunology, 8, 1650.
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4

Identification of Candida auris Isolates

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Respiratory specimens of the C. auris patient were investigated with direct examination using calcofluor staining (BD Biosciences) in KOH (10%) and culture on BBL Chromagar (BD Biosciences) for 5 days at 35°C on malt agar extract (VWR) with gentamicin and chloramphenicol for 10 days at 30°C and Sabouraud dextrose agar with gentamicin and chloramphenicol (Bio-Rad) for 3 weeks. Of note, swab specimens were investigated with just the BBL Chromagar (BD Biosciences) for 5 days. White colonies on BBL were identified using the MALDI-TOF Bruker microFlex system (Bruker Daltonics, Bremen, Germany) with the MBT Compass IVD 4.2 database and the Vitek MS system (bioMérieux, Marcy l’Etoile, France) with the Vitek MS v1.6.0 database.
Alanio A., Snell H.M., Cordier C., Desnos-Olivier M., Dellière S., Aissaoui N., Sturny-Leclère A., Da Silva E., Eblé C., Rouveau M., Thégat M., Zebiche W., Lafaurie M., Denis B., Touratier S., Benyamina M., Dudoignon E., Hamane S., Cuomo C.A, & Dépret F. (2022). First Patient-to-Patient Intrahospital Transmission of Clade I Candida auris in France Revealed after a Two-Month Incubation Period. Microbiology Spectrum, 10(5), e01833-22.
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5

Mycological Diagnosis of Tinea Capitis

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Diagnosis of TC was established at the LPM/ADUH in Dakar, on the basis of mycological examination including direct microscopy and culture as previously described [7 (link)]. Microscopic direct examination of all specimens was carried out in 20% KOH mount. All specimens were cultured on two plates/tubes, one containing Sabouraud dextrose agar (SDA) supplemented with chloramphenicol (Bio-Rad, Paris, France), and the other one containing SDA supplemented with chloramphenicol plus cycloheximide (Bio-Rad, France). Cultures were incubated at 25–30 °C and evaluated for growth after 48 h and then once weekly for a month. Positive specimens for dermatophytes were identified according to three criteria: growth rate, macroscopic and microscopic characteristics of colonies and sometimes on biochemical characteristics such as a urease test [8 ,9 (link)].
Ndiaye M., Sacheli R., Diongue K., Adjetey C., Darfouf R., Seck M.C., Badiane A.S., Diallo M.A., Dieng T., Hayette M.P, & Ndiaye D. (2021). Evaluation of the Multiplex Real-Time PCR DermaGenius® Assay for the Detection of Dermatophytes in Hair Samples from Senegal. Journal of Fungi, 8(1), 11.
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6

Identification of Candida auris Isolates

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Respiratory specimens of the C. auris patient were investigated with direct examination using calcofluor staining (BD Biosciences) in KOH (10%) and culture on BBL Chromagar (BD Biosciences) for 5 days at 35°C on malt agar extract (VWR) with gentamicin and chloramphenicol for 10 days at 30°C and Sabouraud dextrose agar with gentamicin and chloramphenicol (Bio-Rad) for 3 weeks. Of note, swab specimens were investigated with just the BBL Chromagar (BD Biosciences) for 5 days. White colonies on BBL were identified using the MALDI-TOF Bruker microFlex system (Bruker Daltonics, Bremen, Germany) with the MBT Compass IVD 4.2 database and the Vitek MS system (bioMérieux, Marcy l’Etoile, France) with the Vitek MS v1.6.0 database.
Alanio A., Snell H.M., Cordier C., Desnos-Olivier M., Dellière S., Aissaoui N., Sturny-Leclère A., Da Silva E., Eblé C., Rouveau M., Thégat M., Zebiche W., Lafaurie M., Denis B., Touratier S., Benyamina M., Dudoignon E., Hamane S., Cuomo C.A, & Dépret F. (2022). First Patient-to-Patient Intrahospital Transmission of Clade I Candida auris in France Revealed after a Two-Month Incubation Period. Microbiology Spectrum, 10(5), e01833-22.
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