Tp53fl fl

Manufactured by Jackson ImmunoResearch

Sourced in Montenegro, China

Tp53fl/fl is a laboratory product that contains a genetic construct designed to allow for the conditional knockout of the p53 gene. The construct includes loxP sites flanking the p53 gene, which enables the removal of the gene when exposed to Cre recombinase. This product is intended for use in research applications involving the study of p53 function and its role in cellular processes.

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Lab products found in correlation

Rosa lsl tdtomato, by Jackson ImmunoResearch (1 mentions) Kraslsl g12d, by Jackson ImmunoResearch (1 mentions) Lkb1fl fl, by Jackson ImmunoResearch (1 mentions) C57bl 6 mice, by GemPharmatech (1 mentions)

2 protocols using tp53fl fl

Mouse Models for Liver Cancer

Cited 1 time

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All mice were handled in accordance with the guidelines of the Institutional Animal Care and Use Committee at University of Texas Southwestern (UTSW). TG-shAnln and E2f8 KO mice were previously reported.^{15 (link)}Tp53^fl/fl and Rosa-LSL-tdTomato mice were from Jackson Laboratories (Bar Harbor, ME). WT C3H/HeJ mice were used because this is a strain that is prone to liver cancer development. All experiments were done in an age- and sex-controlled fashion unless otherwise noted.

Lin Y.H., Zhang S., Zhu M., Lu T., Chen K., Wen Z., Wang S., Xiao G., Luo D., Jia Y., Li L., MacConmara M., Hoshida Y., Singal A., Yopp A., Wang T, & Zhu H. (2020). “Mice With Increased Numbers of Polyploid Hepatocytes Maintain Regenerative Capacity But Develop Fewer Hepatocellular Carcinomas Following Chronic Liver Injury”. Gastroenterology, 158(6), 1698-1712.e14.

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Genetically Engineered Mouse Models

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Kras^LSL‐G12D/+ (#0 08179), Tp53^fl/fl (#0 08462), Lkb1^fl/fl (#01 4143) mice were purchased from the Jackson Laboratory as previously described.^[⁴³^] C57BL/6 mice were purchased from GemPharmatech Co., Ltd (Nanjing, China). Il1f9^+/− (#032395‐UCD) and Il1f5^+/− (#032393‐UCD) mice were obtained from the Mutant Mouse Resource and Research Center (MMRRC) and were crossed with C57BL/6 mice for six generations before subsequent studies. The Il1f9^−/‐ and Il1f5^−/− mice on C57BL/6 mice background were crossed with Kras^LSL‐G12D/+Tp53^fl/fl (KP) and Kras^LSL‐G12D/+Lkb1^fl/fl (KL) to obtain Kras^LSL‐G12D/+Tp53^fl/flIl1f9^−/− (KP9) or Kras^LSL‐G12D/+Tp53^fl/flIl1f5^−/‐ (KP5) and Kras^LSL‐G12D/+Lkb1^fl/flIl1f9^−/− (KL9) or Kras^LSL‐G12D/+Lkb1^fl/flIl1f5^−/‐ (KL5) mice for maintenance and experiments. All mice were housed in the specific pathogen‐free animal facility at Wuhan University with a 12‐hour dark/12‐hour light cycle and fed with standard food and water. All animal experiments were performed in accordance with protocols approved by the Institutional Animal Care and Use Committee of Wuhan University.

Wang P., Yang W., Guo H., Dong H., Guo Y., Gan H., Wang Z., Cheng Y., Deng Y., Xie S., Yang X., Lin D, & Zhong B. (2021). IL‐36γ and IL‐36Ra Reciprocally Regulate NSCLC Progression by Modulating GSH Homeostasis and Oxidative Stress‐Induced Cell Death. Advanced Science, 8(19), 2101501.

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