Short hairpin tau and rna ctrl vectors

pcDNA3-Tau4R and Tau-GFP have been described elsewhere [14 (link),23 (link),24 (link)]. pimEJ5GFP was a gift from Jeremy Stark (Addgene plasmid # 44026) (Watertown, MA, USA) [25 (link)]. pDRGFP (Addgene plasmid # 26475) and pCBASceI (Addgene plasmid # 26477) were a gift from Maria Jasin [26 (link)]. Short hairpin Tau and RNA Ctrl vectors were purchased from Santacruz. Doxorubicin, cisplatin, oxaliplatin, 6-thioguanine, and hypoxanthine were purchased from Sigma-Aldrich (Saint-Louis, MO, USA), and bleomycin from Calbiochem (from Sigma, St. Louis, MO, USA). Cells were exposed to ionizing radiation (IR) using an X-ray machine (Clinac23X, Varian Medical Systems, Palo Alto, CA, USA).

pGEX vectors encoding Tau and Tau-deletion mutants and pGEX-CBP (aa 1202–1848) fused to GST tag were a kind gift from J.C. Lambert (INSERM U1167, Lille, France) and C. Smet-Nocca (UMR8576, Villeneuve d’Ascq, France), respectively. GFP-HP1β was a gift from Tom Misteli (Addgene plasmid # 17651). pGL4.31(luc2P/GAL4 UAS/Hygro) was purchased from Promega. Short hairpin Tau and RNA Ctrl vectors were purchased from Santacruz. pcDNA3-Tau4R has been described elsewhere (Lippens et al., 2004 (link); Chauderlier et al., 2018 (link)). GAL4-Tau4R was obtained by inserting an in-frame Tau1N4R (referred to as Tau4R) cDNA isoform into the pM GAL4 DNA-BD cloning vector (Clontech). Purified histones and recombinant histones were obtained from EpiCypher and New England Biolabs, respectively. TSA and BIX 01294 (Sigma) were reconstituted in dimethylsulfoxide and Tetracycline (Sigma) in ethanol.