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Jf635 haloligand

Manufactured by Thermo Fisher Scientific
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The JF635-HaloLigand is a fluorescent dye conjugated to a HaloTag ligand. The HaloTag ligand allows for covalent labeling of HaloTag fusion proteins. The JF635-HaloLigand emits fluorescence in the far-red region of the visible spectrum.

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Lab products found in correlation

Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Picogreen dsdna reagent, by Thermo Fisher Scientific (2 mentions) Mitotracker deep red fm, by Thermo Fisher Scientific (2 mentions) Cellmask orange, by Thermo Fisher Scientific (2 mentions) Hoechst 34580, by Thermo Fisher Scientific (2 mentions) Alexa fluor 555 phalloidin, by Thermo Fisher Scientific (2 mentions) Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (2 mentions) Sir lysosome, by Cytoskeleton (2 mentions) Sir actin, by Cytoskeleton (2 mentions)

2 protocols using jf635 haloligand

1

Multimodal Fluorescent Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
MitoTracker Red CMXRos (100 nM; CST, 9082), MitoTracker Deep Red FM (100 nM; Invitrogen, M22426), TMRE (Life Technologies, T-669), SiR-Lysosome (1 μM; Cytoskeleton inc., CY-SC012), SiR-Actin (1 μM; Cytoskeleton inc., CY-SC001), PicoGreen dsDNA Reagent (1:10000; Invitrogen, P7581), Hoechst 33342 (1:1000; Invitrogen, H3570), Hoechst 34580 (1:1000; Invitrogen, H21486), Alexa Fluor 488 Phalloidin (1:40; Invitrogen, A12379), Alexa Fluor 555 Phalloidin (1:40; Invitrogen, A34055), Alexa Fluor 568 Phalloidin (1:40; Invitrogen, A12380), CellMask Orange (1:2000; Invitrogen, C10045), JF635-HaloLigand (100 nM; gift from L. Lavis, Ashburn, VA).
Moore A.S., Coscia S.M., Simpson C.L., Ortega F.E., Wait E.C., Heddleston J.M., Nirschl J.J., Obara C.J., Guedes-Dias P., Boecker C.A., Chew T.L., Theriot J.A., Lippincott-Schwartz J, & Holzbaur E.L. (2021). Actin cables and comet tails organize mitochondrial networks in mitosis. Nature, 591(7851), 659-664.
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2

Multimodal Fluorescent Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
MitoTracker Red CMXRos (100 nM; CST, 9082), MitoTracker Deep Red FM (100 nM; Invitrogen, M22426), TMRE (Life Technologies, T-669), SiR-Lysosome (1 μM; Cytoskeleton inc., CY-SC012), SiR-Actin (1 μM; Cytoskeleton inc., CY-SC001), PicoGreen dsDNA Reagent (1:10000; Invitrogen, P7581), Hoechst 33342 (1:1000; Invitrogen, H3570), Hoechst 34580 (1:1000; Invitrogen, H21486), Alexa Fluor 488 Phalloidin (1:40; Invitrogen, A12379), Alexa Fluor 555 Phalloidin (1:40; Invitrogen, A34055), Alexa Fluor 568 Phalloidin (1:40; Invitrogen, A12380), CellMask Orange (1:2000; Invitrogen, C10045), JF635-HaloLigand (100 nM; gift from L. Lavis, Ashburn, VA).
Moore A.S., Coscia S.M., Simpson C.L., Ortega F.E., Wait E.C., Heddleston J.M., Nirschl J.J., Obara C.J., Guedes-Dias P., Boecker C.A., Chew T.L., Theriot J.A., Lippincott-Schwartz J, & Holzbaur E.L. (2021). Actin cables and comet tails organize mitochondrial networks in mitosis. Nature, 591(7851), 659-664.
+ Open protocol
+ Expand

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