The recombinant humanised monoclonal HER2 antibody trastuzumab (a concentration of 15 µg/mL was selected as indicated elsewhere [24 (link)]) (Herceptin, Genentech, San Francisco, CA, United States) was supplied by the pharmacy of our hospital; BEZ235 (S1009), everolimus (S1120) and TAK-228 (S2811) were obtained from Selleckchem (Selleckchem Spain, Madrid, Spain).
Everolimus s1120
Manufactured by Selleck Chemicals
Sourced in United States, China
Everolimus (S1120) is a chemical compound used as a reference standard and research tool. It is a potent inhibitor of the mammalian target of rapamycin (mTOR) pathway, which is involved in cellular growth and proliferation. Everolimus (S1120) is commonly used in cell-based assays and biochemical studies to investigate the role of the mTOR signaling cascade.
Automatically generated - may contain errors
Lab products found in correlation
Bez235 s1009, by Selleck Chemicals (1 mentions)
Trastuzumab, by Genentech (1 mentions)
Herceptin, by Genentech (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Abt 737 s1002, by Selleck Chemicals (1 mentions)
3 protocols using everolimus s1120
1
Trastuzumab and PI3K/mTOR Inhibitors
2
Western Blot Antibody Reagents for Cell Signaling
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Antibodies for CTPS (15914-1-AP), Raptor (20984-1-AP), Rictor (27248-1-AP), S6K1 (14485-1-AP), ATF4 (10835-1-AP) and MTHFD2 (12270-1-AP) were purchased from ProteinTech (China). Antibodies for mTOR (#2983S) and Phospho-S6K1 (#9205) were purchased from Cell Signaling Technology (USA). Antibodies for c-Myc (ab32072) and β-Actin (ab6276) were purchased from Abcam (USA). Antibody for HA (sc-7392) and normal mouse IgG (sc-2025) were from Santa Cruz Biotechnology (USA). Antibody for α-tubulin (T5168) was purchased from Sigma-Aldrich (USA). Antibodies for Drosophila CTPS (134457 and 33304) were purchased from Santa Cruz. Antibody for Hu-li tao shao (Hts) (7H9 1B1) was purchased from Developmental Studies Hybridoma Bank (USA). Ramaycin (S1039) and everolimus (S1120) were from Selleck Chemicals (China).
3
Human and Mouse Renal Cell Carcinoma Cell Lines
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The human RCC cell lines A-498, Caki-2, Caki-1, ACHN, HEK-293 and mouse murine RCC cell line RenCa were purchased from American Type Culture Collections (ATCC). All cell lines were cultured in appropriate media supplemented with 10% FBS (Gibco), 100 units/ml penicillin (Gibco), and 100 μg/ml streptomycin (Gibco) and maintained in a humidified incubator at 37ºC and 5% CO2.
Everolimus (S1120) and ABT-737 (S1002) were purchased from Selleck Chemicals. Stock concentrations were prepared in DMSO and stored according to the manufacturer's protocol.
Everolimus (S1120) and ABT-737 (S1002) were purchased from Selleck Chemicals. Stock concentrations were prepared in DMSO and stored according to the manufacturer's protocol.
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