MRI images were acquired using a Bruker Avance III System (Bruker BioSpin, Ettlingen, Germany) with a 14.1-T superconducting magnet with a 12 cm diameter gradient providing 100 G/cm with a 150 μs rise time. A custom-made transceiver surface coil (8 mm in diameter) covering the whole brain of mice was applied for imaging. Functional scans were acquired using a 2D echo-planar imaging (EPI) sequence, with the following parameters: TR = 1 s, TE = 8 ms, FOV = 12 × 12 mm, 40 × 40 acquisition matrix, 24 slices, 0.3 × 0.3 × 0.5 mm3 resolution, 210 TRs total time. The anatomical RARE (rapid acquisition with relaxation enhancement sequence) images were acquired using the same geometry as functional scans with the following parameters: TR = 2.5 s, TE = 7 ms, FOV = 12 × 12 mm, 96 × 96 acquisition matrix, 24 slices, 0.125 mm2 in-plane resolution, 0.5 mm slice thickness, 6 × RARE factor, 4 repetitions. The magnetic field homogeneity was corrected using the FASTMAP scout. A well-trained mouse was used to adjust the magnetic field homogeneity, which was also used as a reference for imaging other mice. The duration for multi-order shimming and positioning adjustment is ~17 min.
Avance 3 system
Manufactured by Bruker
Sourced in Germany
The AVANCE III system is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for advanced analytical and research applications in the field of chemistry, materials science, and life sciences. It provides a robust and flexible platform for acquiring and analyzing NMR data with high sensitivity and resolution.
Automatically generated - may contain errors
Lab products found in correlation
Ir prestige 21 spectrophotometer, by Shimadzu (1 mentions)
Cryogenic triple resonance probe, by Bruker (1 mentions)
Avance spectrometer, by Bruker (1 mentions)
1260 system, by Agilent Technologies (1 mentions)
Exactive plus orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions)
C18 column, by Agilent Technologies (1 mentions)
Cryoprobe, by Bruker (1 mentions)
Nicolet is50 ftir spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Avance 600 system, by Bruker (1 mentions)
Dsc q200, by TA Instruments (1 mentions)
Optilab t rex refractive index detector, by Wyatt Technology (1 mentions)
8 protocols using avance 3 system
1
High-Field MRI Imaging of Mouse Brain
2
Characterization of Ionic Liquid Precursors
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All the chemicals and solvents were purchased from commercial suppliers (Aldrich, Merck) and used as received without further purification except for choline chloride (ChCl), which was dried at 130 °C in vacuum and stored under nitrogen prior to use. Melting points, reported without correction, were measured using a Fisher–Jhones apparatus. The Fourier transform infrared spectra were obtained using a Shimadzu IR prestige 21 spectrophotometer (Columbia, MD, USA). 1H and 13C NMR spectra were recorded using a Bruker AVANCE III system operating at 400 MHz, using residual and deuterated solvent peaks of CDCl3 (δH 7.26; δC 77.0) and DMSO (δH 2.50; δC 39.5) as reference standards. The peak patterns are indicated as follows: s, singlet; d, doublet; t, triplet; m, multiplet; and q, quartet. The coupling constants, J, are reported in hertz (Hz).
3
Solid-State NMR Analysis of Digested Samples
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The solution 1H NMR spectra of digested samples (dissolved in dimethyl sulfoxide d6) were recorded on a 600-MHz Avance spectrometer equipped with a triple resonance cryogenic probe using a simple 1D pulse sequence (Bruker, Germany). ssNMR measurements were conducted on a 400-MHz Avance III system equipped with a 4-mm HXY triple resonance MAS probe (Bruker, Germany). The samples were ground by an agate mortar into a homogeneous powder, transferred to a zirconia NMR tube, and analyzed by the spectrometer.
4
HPLC-Orbitrap Analysis of Genistein Metabolites
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HPLC
was carried out using an Agilent 1260 system with a DAD detector,
equipped with a SILGREEN C18 column (4.6 × 250 mm2, 5 μm). The sample volumes of 50 μL were injected and
a flow rate of 1 mL/min was set. The reaction mixtures were separated
using a gradient of HPLC grade water (solvent A) and HPLC grade acetonitrile
(solvent B) as the mobile phase according to the following eluting
program: 0–35 min, linear gradient from 5 to 50% B (v/v); 35–36
min, linear gradient from 50 to 100% B (v/v); 36–40min, held
at 100% B (v/v); 40–42, 100 to 5% B (v/v); and 42–45
min, kept at 5% B (v/v). C18 column was kept at ambient temperature,
and the peaks were detected at a wavelength of 265 nm.
Hydroxylated
products of genistein collected from the C18 column were injected
into a Thermo Exactive Plus Orbitrap mass spectrometer for high-resolution
mass spectrometry (HR-MS) measurement. An ESI source was employed
in a positive ionization mode. Full MS scans were acquired over the
range of m/z 100–1500.
NMR spectra were
recorded on a Bruker 600 MHz AVANCE III system,
as introduced in our previous reports.37 (link)−39 (link) Briefly, the samples
were run in deuterated methanol (CD3OD) at 25 °C. Chemical shifts
were recorded in δ (ppm) with the residual methyl signals in
CD3OD as the references. The NMR assignments of hydroxygenisteins
were based on 1H and 13C chemical shifts.
was carried out using an Agilent 1260 system with a DAD detector,
equipped with a SILGREEN C18 column (4.6 × 250 mm2, 5 μm). The sample volumes of 50 μL were injected and
a flow rate of 1 mL/min was set. The reaction mixtures were separated
using a gradient of HPLC grade water (solvent A) and HPLC grade acetonitrile
(solvent B) as the mobile phase according to the following eluting
program: 0–35 min, linear gradient from 5 to 50% B (v/v); 35–36
min, linear gradient from 50 to 100% B (v/v); 36–40min, held
at 100% B (v/v); 40–42, 100 to 5% B (v/v); and 42–45
min, kept at 5% B (v/v). C18 column was kept at ambient temperature,
and the peaks were detected at a wavelength of 265 nm.
Hydroxylated
products of genistein collected from the C18 column were injected
into a Thermo Exactive Plus Orbitrap mass spectrometer for high-resolution
mass spectrometry (HR-MS) measurement. An ESI source was employed
in a positive ionization mode. Full MS scans were acquired over the
range of m/z 100–1500.
NMR spectra were
recorded on a Bruker 600 MHz AVANCE III system,
as introduced in our previous reports.37 (link)−39 (link) Briefly, the samples
were run in deuterated methanol (CD3OD) at 25 °C. Chemical shifts
were recorded in δ (ppm) with the residual methyl signals in
CD3OD as the references. The NMR assignments of hydroxygenisteins
were based on 1H and 13C chemical shifts.
5
Peptide Characterization via NMR
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HS-Cn+1-His-Phe
was dissolved in 5% CD3OD–95% H2O mixing
solvent to afford 50 μM at concentration, and the chemical shift
signal in the range of 1.20–1.34 ppm was collected by an 850
MHz/AVANCE III system (Bruker, Switzerland).
was dissolved in 5% CD3OD–95% H2O mixing
solvent to afford 50 μM at concentration, and the chemical shift
signal in the range of 1.20–1.34 ppm was collected by an 850
MHz/AVANCE III system (Bruker, Switzerland).
6
In Vivo Mouse Brain MRI Protocols
7
Mechanical Properties of DPI-HFP/Silicone Composites
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To determine the mechanical properties of the DPI-HFP/silicone composites, uniaxial tensile tests were performed using an Instron 3343 universal testing machine (Instron, USA) with a fixed receding strain rate of 10%/s. We used a VL-215 L model (Vilber Lourmat, France) consisting of a 15 W tube, which emits an intensity of 2300 μW/cm3 at a distance of 15 cm, for the UV lamp required for the decomposition reaction. A Nicolet iS50 FTIR spectrophotometer (Thermo Fisher Scientific, USA) was used for decomposition chemistry analysis. 29Si- and 1H-solid-state NMR data were obtained using a 500 MHz Avance III system (Bruker, Germany), and solution-state 29Si-NMR data were obtained using a 600 MHz Avance 600 system (Bruker, Germany) with tetrahydrofuran as the solvent. GPC measurements were obtained with a Shodex SEC LF-804 column with a Wyatt OptiLab T-rEx refractive index detector, with chloroform as the solvent. Decomposition kinetics were measured using a photo-DSC system comprising a DSC-Q200 (TA Instruments, USA) and an Omnicure-s2000 spot-cure light source (Excelitas, USA).
8
Solid-State NMR Spectroscopy of Materials
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Solid-state nuclear magnetic resonance
(NMR) spectra were acquired on a Bruker AVANCE III system using a
wide-bore 9.4 T magnet, corresponding to Larmor frequencies of 400.2
MHz for 1H, 100.6 MHz for 13C, and 79.5 MHz
for 29Si with 7 mm-diameter zirconia rotors and at a magic
angle spinning (MAS) rate of 4 kHz. 1H–29Si and 1H–13C cross polarization MAS
NMR spectra were acquired to boost the sensitivity, with relatively
long contact times (5 and 2 ms, respectively) to minimize the dependency
of the spectral intensities on the 1H–X distance,
but the spectra can only be considered in a semi-quantitative approach
at best.
(NMR) spectra were acquired on a Bruker AVANCE III system using a
wide-bore 9.4 T magnet, corresponding to Larmor frequencies of 400.2
MHz for 1H, 100.6 MHz for 13C, and 79.5 MHz
for 29Si with 7 mm-diameter zirconia rotors and at a magic
angle spinning (MAS) rate of 4 kHz. 1H–29Si and 1H–13C cross polarization MAS
NMR spectra were acquired to boost the sensitivity, with relatively
long contact times (5 and 2 ms, respectively) to minimize the dependency
of the spectral intensities on the 1H–X distance,
but the spectra can only be considered in a semi-quantitative approach
at best.
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