Largal ultra

The no. 10 k-file (Dentsply Maillefer, Ballaigues, Switzerland) was used to establish the canal patency. All the samples were prepared to the size A1 (size 40, 0.04 taper) Neoniti (Neolix, Châtres-la-Forêt, France) rotary file. The canals were irrigated during instrumentation procedure with 3% sodium hypochlorite (Novo Dental Products Pvt. Ltd, Mumbai, India) saline and 17% ethylenediaminetetraacetic acid (Largal Ultra, Septodont, Saint Maitre, France) throughout the shaping and cleaning procedure and dried with paper points (Kerr Corp., Romulus, MI) followed by obturation with the same size and taper gutta-percha with Cold lateral compaction. GuttaFlow was used as root canal sealer (Coltene/Whaledent, Langenau, Germany) in all the samples.
The teeth were temporized using Cavit G (3M ESPE, Germany) and stored at 37°C in 100% humidity for 1 week to allow complete sealing of the sealer. Teeth were radiographed in buccolingual and mesiodistal directions to assess the radiographic adequacy of root filling, using the following criteria: reaching the working length, uniform radiopacity, and no voids.

The specimens were divided into two groups (n = 60) depending on the irrigating solution. The irrigating solutions used were QMix (Dentsply Tulsa dental, USA) and EDTA (Largal Ultra, Septodont). Samples were further divided depending on temperature of irrigating solution. The irrigants (QMix and EDTA) (n = 30) were pre heated to the temperature 60°C using water bath. Normal QMIX and EDTA served as control.
In both the groups, post spaces were irrigated with the mentioned irrigating solutions and was activated with the two auxiliary techniques. For the ultrasonic subgroups, the ultrasonic D₁tip (Acteon, Merignac, France) was used, running all 10 laps around them, with 10% of power. For the laser subgroups, the diode laser (Biolase, EpicX, USA) was irradiated in the root canal with the following parameters: 1.5 W output power, 20 Hz frequency, 238.85 J/cm² , and light in continuous mode. A 200 mm fiber optic tip was introduced into the post space. All the samples were irrigated with normal saline as a final irrigant. The canals were dried with paper points. The fiberglass posts were (Selfpost, Medicept dental) cemented to the root canal by using Dual cure Resin cement (Maxcem Elite, Kerr Corporation). The specimens were kept at 37°C and relative humidity for 7 days.