Zb wax capillary column

The FAME content was determined using GC-FID (Agilent Technologies, Santa Clara, CA, USA, 6890 N) equipped with a Zebron ZB-WAX capillary column (30 m × 0.25 mm × 0.25 µm) and a flame ionization detector (FID, Agilent Technologies 7683). The gas carrier was helium with a constant flow rate of 1 mL min⁻¹. The injection volume was 1 µL in a 1:20 split mode. The GC oven temperature program consists of three stages: 60 °C for 1 min, 60 to 200 °C (rate 10 °C min⁻¹, 2 min), 200 to 220 °C (5 °C min⁻¹, 20 min). The temperature of the injector and detector was set to 250 °C. FAs in samples were identified by comparing their retention time with that of the Supelco FAME standard mixture.

The FAMEs content was determined by GC-FID (Agilent Technologies, 6890N GC, Santa Clara, CA, USA) equipped with a ZB-WAX capillary column (30 m × 0.25 mm × 0.25 µm) and a flame ionization detector (FID, Agilent Technologies 7683, Santa Clara, CA, USA). The gas carrier was helium (purity ≥ 99.999%) with a constant 1 mL/min flow rate. The injection volume was 1 µL in 1:20 split mode. The GC oven temperature program consists of three stages: 60 °C for 1 min, 60 to 200 °C (rate 10 °C/min, kept for 2 min), from 200 to 220 °C (rate 5 °C/min, kept for 20 min). The temperature of the injector and detector was set to 250 °C. The data processing was performed with the Agilent OpenLab CDS Chemstation software C.01.10 running under Windows (Microsoft, Santa Clara, CA, USA). The FAs in samples were identified by comparing their retention time with that of the Supelco FAME standard mixtures standard mixture.