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C caspase 3

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C-Caspase-3 is a laboratory reagent used for the detection and quantification of active caspase-3, a key enzyme involved in the apoptosis (programmed cell death) pathway. The product provides a reliable and specific tool for researchers studying cell death mechanisms.

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Lab products found in correlation

C caspase 8, by Proteintech (2 mentions) C caspase 9, by Proteintech (2 mentions) β actin, by Proteintech (1 mentions) Artesunate, by Merck Group (1 mentions) Survivin, by Proteintech (1 mentions) P akt, by Proteintech (1 mentions) Eclipse e100, by Nikon (1 mentions) Odyssey fluorescence scanner, by LI COR (1 mentions) Gapdh, by Proteintech (1 mentions)

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Caspase-3 (181 citations)

4 protocols using c caspase 3

1

Apoptosis Pathway Regulation

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Artesunate and dimethyl sulfoxide (DMSO) were made a purchase from Sigma (USA). Antibodies of p-AKT, AKT, Survivin, c-caspase-3, and β-actin were all obtained from Proteintech.
Zhang W., Ning N, & Huang J. (2022). Artesunate Suppresses the Growth of Lung Cancer Cells by Downregulating the AKT/Survivin Signaling Pathway. BioMed Research International, 2022, 9170053.
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2

Histological and IHC Analysis of Prostate

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The prostates were immediately fixed in 4% paraformaldehyde at 4 °C for 24 h and then in 50% ethanol before processing for histological studies. Serial sections (5 μm thickness; 1 section per slide) were performed for histological and immunohistochemistry (IHC) analyses. For histology, sections were subjected to hematoxylin and eosin (H&E) staining and were examined using a light microscope (Nikon Eclipse E100, Tokyo, Japan). Fields were selected following systematic random sampling scheme, and the epithelial thickness was measured using ImageJ software. For IHC studies, after deparaffinization and dehydration, sections were washed with 3% H2O2 for 10 min at room temperature, and non-specific binding of immunoglobulins was blocked with 2% normal goat serum (diluted 1:10 in PBS) for 30 min at room temperature. Sections were boiled for 30 min in critic acid (pH 6.0) for antigen retrieval. After blocking, sections were incubated overnight with primary antibodies, as follows: Ki-67, C-Caspase-3, C-Caspase-8, C-Caspase-9 (Proteintech Group, Inc). Images were acquired using a light microscope (Nikon Eclipse E100, Tokyo, Japan).
Wang S., Li Y., Li W., Zhang K., Yuan Z., Cai Y., Xu K., Zhou J, & Du Z. (2020). Curcuma oil ameliorates benign prostatic hyperplasia through suppression of the nuclear factor-kappa B signaling pathway in rats. Journal of ethnopharmacology, 279, 113703.
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3

Protein Extraction and Immunoblotting Protocol

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Cells were collected and lysed in radioimmunoprecipitation assay buffer (Beyotime; 50 mM tris-HCl (pH 7.4), 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 150 mM NaCl, 2 mM EDTA, and 50 mM NaF) including a cocktail of protease (Topscience; 1 mM AEBSF.HCl, 1 μM aprotinin, 50 μM bestatin, 15 μM E-64, 20 M leupeptin, 15 μM pepstatin A) and phosphatase (Topscience; 1 mM sodium fluoride, 1 mM sodium orthovanadate, 1.15 mM sodium molybdate, 4 mM sodium tartrate, 2 mM imidazole, 25 M (−)-p-bromotetramisole oxalate, 5 μM cantharidin, 5 nM microcystin-LR) inhibitors. Immunoblots were performed following the standard protocol and detected using an Odyssey fluorescence scanner (LI-COR). All experiments were performed in triplicate. Antibodies used are KIF11/KSP/Eg5 (1:1000; #23333-1-AP, Proteintech), p53 (1:1000; #10442-1-AP, Proteintech), c-Caspase3 (1:1000; #19677-1-AP, Proteintech), GAPDH (1:1000; #10494-1-AP, Proteintech), p-CHK1 (1:1000, #12302, CST), p-P53 (1:1000, #2521, CST), γ-H2A.x (1:1000; #9718, CST), PARP (1:1000; #9532, CST).
Shi Y., Cui X., Jiang T., Pan Y., Lin Y., Feng X., Ding Z., Yang C., Tan Y., Wang H, & Dong L. (2022). The therapeutic effect of KSP inhibitors in preclinical models of cholangiocarcinoma. Cell Death & Disease, 13(9), 799.
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4

Testosterone Propionate and Finasteride Effects

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Testosterone propionate was purchased from Ningbo Second Hormone Factory and finasteride was purchased from CONBA Co. LTD, Zhejiang. The Rat dihydrotestosterone (DHT), 5α-reductase, IL-1β, IL-6 and TNF-α ELISA kits were provided by Jiangsu Enzyme Standard Biotechnology Co., Ltd (Jiangsu, China). Protein Extraction Solution was purchased from Dingguo Changsheng Biotechnology Co., Ltd (Beijing). Antibodies for Ki-67, C-Caspase-3, C-Caspase-8 and C-Caspase-9 were purchased from Proteintech Group, Inc (American).
Wang S., Li Y., Li W., Zhang K., Yuan Z., Cai Y., Xu K., Zhou J, & Du Z. (2020). Curcuma oil ameliorates benign prostatic hyperplasia through suppression of the nuclear factor-kappa B signaling pathway in rats. Journal of ethnopharmacology, 279, 113703.
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