Western blot was performed on neuronal lysates using 15 μg of protein. The protein samples were loaded on 4–20% TGX gels (Bio-Rad) at 150V for 1 h. All polyvinylidene fluoride membranes were probed with anti-Lamin A + C (Abcam; 1:200), tubulin 1a (Abcam 1), and vinculin (Abcam 1:10,000) as primary antibodies. Secondary antibodies used were goat antimouse or antirabbit, all coupled to horseradish peroxidase (1:10,000; Sigma-Aldrich). Protein selection for western blot validation was based on Limma statistics results (i.e., FC and p-value) and published literature regarding neurodegeneration and CATB. Analysis and quantification of western blot bands were performed by dividing the volume intensity of each protein band by the volume intensity of the loading control band. Cyclophilin B (Abcam, 1:5000) was used as the loading control (internal control). Restore PLUS Mild Western Blot Stripping Buffer (Thermo Fisher Scientific, MA, USA) was used to reprobe membranes for 30 min at 37 °C, followed by washing, blocking, and reprobing with a different antibody.
Tubulin 1a
Manufactured by Abcam
Tubulin 1a is a protein that is a key component of microtubules, which are part of the cytoskeleton in eukaryotic cells. It plays a crucial role in cellular processes such as cell division, intracellular transport, and the maintenance of cell structure.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tubulin 1a
1
Western Blotting of Neuronal Lysates
2
Western Blot Analysis of Neuronal Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot
was performed on neuronal lysates using 15 μg of protein. The
protein samples were loaded on 4–20% TGX gels (Bio-Rad) at
150V for 1 h. All polyvinylidene fluoride membranes were probed with
anti-Lamin A + C (Abcam; 1:200), tubulin 1a (Abcam 1), and vinculin
(Abcam 1:10,000) as primary antibodies. Secondary antibodies used
were goat antimouse or antirabbit, all coupled to horseradish peroxidase
(1:10,000; Sigma-Aldrich). Protein selection for western blot validation
was based on Limma statistics results (i.e., FC and p-value) and published literature regarding neurodegeneration and
CATB. Analysis and quantification of western blot bands were performed
by dividing the volume intensity of each protein band by the volume
intensity of the loading control band. Cyclophilin B (Abcam, 1:5000)
was used as the loading control (internal control). Restore PLUS Mild
Western Blot Stripping Buffer (Thermo Fisher Scientific, MA, USA)
was used to reprobe membranes for 30 min at 37 °C, followed by
washing, blocking, and reprobing with a different antibody.
was performed on neuronal lysates using 15 μg of protein. The
protein samples were loaded on 4–20% TGX gels (Bio-Rad) at
150V for 1 h. All polyvinylidene fluoride membranes were probed with
anti-Lamin A + C (Abcam; 1:200), tubulin 1a (Abcam 1), and vinculin
(Abcam 1:10,000) as primary antibodies. Secondary antibodies used
were goat antimouse or antirabbit, all coupled to horseradish peroxidase
(1:10,000; Sigma-Aldrich). Protein selection for western blot validation
was based on Limma statistics results (i.e., FC and p-value) and published literature regarding neurodegeneration and
CATB. Analysis and quantification of western blot bands were performed
by dividing the volume intensity of each protein band by the volume
intensity of the loading control band. Cyclophilin B (Abcam, 1:5000)
was used as the loading control (internal control). Restore PLUS Mild
Western Blot Stripping Buffer (Thermo Fisher Scientific, MA, USA)
was used to reprobe membranes for 30 min at 37 °C, followed by
washing, blocking, and reprobing with a different antibody.
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