Human il 1β elisa kit

Manufactured by BD

Sourced in United States

The Human IL-1β ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-1 beta (IL-1β) levels in various biological samples.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (3 mentions) Sonication probe, by Sonics (2 mentions) Nigericin, by Merck Group (2 mentions) Adenosine triphosphate (atp), by Merck Group (2 mentions) 96 well plate, by Corning (1 mentions) Dansylsarcosine piperidinium salt, by Merck Group (1 mentions) Antibodies against, by Cell Signaling Technology (1 mentions) Anti mouse igg dylight 488, by Abbkine (1 mentions) Anti β actin antibody 66009, by Proteintech (1 mentions) Anti mouse igg dylight 649, by Abbkine (1 mentions) Anti rabbit igg dylight 649, by Abbkine (1 mentions) Gtx128092, by GeneTex (1 mentions) D4d8t, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Phorbol 12 myristate 13 acetate, by Merck Group (1 mentions) Mouse igg, by Thermo Fisher Scientific (1 mentions) Mouse il6, by MultiSciences Biotech (1 mentions) Anti asc sc 271054, by Santa Cruz Biotechnology (1 mentions) Anti il 1β d3u3e, by Cell Signaling Technology (1 mentions) Mouse il 1β elisa kit, by MultiSciences Biotech (1 mentions)

5 protocols using human il 1β elisa kit

Engineered Nanomaterial-Induced IL-1β Release

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Aliquots of 5 × 10⁴ wild type, NLRP3^–/– or ASC^–/– THP-1 cells were seeded in 96-well plates, with each well receiving 100 μL RPMI 1640 medium supplemented with 10% fetal bovine serum. 1 μg/mL phorbol 12-myristate acetate (PMA) was added to prime cells overnight before the addition of ENMs. The ENM particles were sonicated in complete RPMI 1640 medium (c-RPMI 1640), supplemented with 10 ng/mL lipopolysaccharide (LPS), at 32 W for 15 s with a sonication probe (Sonics & Materials, USA) before addition to the cells. IL-1β release into the culture supernatants was detected by a human IL-1β ELISA Kit (BD, CA, USA) in THP-1 cells that were exposed to the ENMs for 6–24 h at the indicated concentrations.

Li R., Ji Z., Qin H., Kang X., Sun B., Wang M., Chang C.H., Wang X., Zhang H., Zou H., Nel A.E, & Xia T. (2014). Interference in Autophagosome Fusion by Rare Earth Nanoparticles Disrupts Autophagic Flux and Regulation of an Interleukin-1β Producing Inflammasome. ACS Nano, 8(10), 10280-10292.

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Quantifying NLRP3 and ASC Mediated IL-1β Production

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IL-1β production was detected in the culture media of THP-1 cells using a human IL-1β ELISA Kit (BD; San Jose, CA, USA). Briefly, aliquots of 5 × 10⁴ wild type, NLRP3^–/– or ASC^–/– THP-1 cells were seeded in 0.1 mL complete medium and primed with 1 μg/mL phorbol 12-myristate acetate (PMA) overnight in 96-well plates (Corning; Corning, NY, USA). Cells were treated with the desired concentration of the particle suspensions made up in complete RPMI 1640 medium, supplemented with 10% fetal bovine serum and 10 ng/mL lipopolysaccharide (LPS). The suspensions were sonicated with a sonication probe (Sonics & Materials) at 32 W for 15 s before adding to the cells.

Li R., Ji Z., Chang C.H., Dunphy D.R., Cai X., Meng H., Zhang H., Sun B., Wang X., Dong J., Lin S., Wang M., Liao Y.P., Brinker C.J., Nel A, & Xia T. (2014). Surface Interactions with Compartmentalized Cellular Phosphates Explain Rare Earth Oxide Nanoparticle Hazard and Provide Opportunities for Safer Design. ACS Nano, 8(2), 1771-1783.

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Quantifying IL-1β Secretion in LPS-primed HMEC-1 Cells

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LPS-primed HMEC-1 cells were grown in 24 well-plates, transfected as described earlier and incubated at 5% CO₂ and 37°C overnight. Further, cell supernatants were harvested at the indicated times post-transfection and analyzed for the presence of IL-1β using an enzyme-linked immunosorbent assay (Human IL-1β ELISA Kit II BD biosciences San Jose CA, USA) according to the manufacturer's instructions, and the concentration of IL-1β of the unknown samples and controls was determined from a standard curve. Samples producing signals higher than that of the highest standard (250 pg/ml) were diluted and re-analyzed. Each sample was run in triplicate and the assay were repeated for at least three times. The absorbance was read at 450 nm within 30 min of stopping reaction.

Shrivastava G., Visoso-Carvajal G., Garcia-Cordero J., Leon-Juarez M., Chavez-Munguia B., Lopez T., Nava P., Villegas-Sepulveda N, & Cedillo-Barron L. (2020). Dengue Virus Serotype 2 and Its Non-Structural Proteins 2A and 2B Activate NLRP3 Inflammasome. Frontiers in Immunology, 11, 352.

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Inflammasome Activation and Cytokine Release

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Phorbol-12-myristate-13-acetate (PMA), LPS, nigericin, ATP, and dansylsarcosine piperidinium salt (DSS) were purchased from Sigma-Aldrich. Caspase-1 inhibitor (VX-765) was purchased from Selleck. Recombinant human IL-1β protein (200-01B) and human IL-1RA (200-01RA) were obtained from Peprotech. Recombinant mouse IL-1β protein (401-ML/CF) and recombinant mouse IL-1RA (480-RM/CF) were purchased from R&D Systems. Trizol reagent was from Invitrogen, and Lipofectamine 2000 was from Invitrogen. The human IL-1β ELISA kit was purchased from BD Biosciences, the mouse IL-1β and tumor necrosis factor (TNF)-α ELISA kits were purchased from R&D Systems, and the LPS ELISA kit was purchased from Expandbio (Beijing, China).
Antibodies against NLRP3 (D4d8T), caspase-1 (D7F10), and IL-1β (D3U3E and 3A6) were purchased from Cell Signaling Technology. Antibody against DENV-NS3 (GTX124252) and DENV-Prm (GTX128092) were purchased from Genetex. Anti-β-actin antibody (66009) was purchased from Proteintech. Antibody against ASC (sc-271054) was purchased from Santa Cruz Biotechnology. Anti-mouse IgG Dylight 649, anti-mouse IgG Dylight 488, anti-rabbit IgG Dylight 649, and anti-rabbit IgG fluorescein isothiocyanate (FITC) were purchased from Abbkine.

Pan P., Zhang Q., Liu W., Wang W., Yu Z., Lao Z., Zhang W., Shen M., Wan P., Xiao F., Shereen M.A., Zhang W., Tan Q., Liu Y., Liu X., Wu K., Liu Y., Li G, & Wu J. (2019). Dengue Virus Infection Activates Interleukin-1β to Induce Tissue Injury and Vascular Leakage. Frontiers in Microbiology, 10, 2637.

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Comprehensive ELISA and qRT-PCR Assays

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Human IL-1β ELISA kit was purchased from BD Biosciences. Human IL-6, human IL-18, mouse IL-6, mouse IL-1β ELISA kit weas purchased from MULTI SCIENCES. PMA, Nigericin, LPS, ATP and dansylsarcosine piperidinium salt (DSS) were purchased from Sigma-Aldrich. Lipofectamine 2000 reagent was purchased from Invitrogen. Trizol reagent was purchased from Ambion.
Anti-NLRP3 (D4d8T), anti-Caspase-1 (D7F10) and anti-IL-1β (D3U3E) antibodies were purchased from Cell Signaling Technology. Anti-NLRP3 (ALX-804-818) were purchased from Enzo Life Science. Anti-SARS-CoV-2-N (A20021) and anti-β-actin (AC026) antibody was purchased from ABclonal. Anti-Flag (F3165), anti-HA (H6908) and anti-GAPDH (G8759) were purchased from Sigma. Anti-ASC (sc-271054) was purchased from Santa Cruz Biotechnology. Rabbit IgG and Mouse IgG were purchased from Invitrogen. Anti-mouse/Rabbit IgG Dylight 649, anti-mouse/Rabbit IgG Dylight cy3, and anti-mouse/Rabbit IgG FITC were purchased from Abbkine.
RNA extract and qRT-PCR Total RNAs were extracted by using Trizol reagent (Invitrogen, CA) according to the manufacturer's instructions. Real-time quantitative reverse transcriptase-PCR (qRT-PCR) was performed using the Roche LC480 and SYBR RT-PCR Kits (Invitrogen). The Real-time PCR primers were designed by Primer-blast, NCBI (www.ncbi.nlm.nih.gov) and their sequences are listed in Table 1.

Pan P., Shen M., Yu Z., Ge W., Chen K., Tian M., Xiao F., Li G., Wang Z., Wang J., Jia Y., Wang W., Wan P., Zhang J., Chen W., Lei Z., Chen X., Luo Z., Zhang Q., Xu M., Li Y., & Wu J. (2020). SARS-CoV-2 N promotes the NLRP3 inflammasome activation to induce hyperinflammation.

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